玉研自研精品 脑立体定位仪：打开大脑奥秘之门的钥匙

• 帕金森氏病动物模型：帕金森氏病是一种常见的神经退行性疾病，严重影响患者的生活质量。在研究帕金森氏病的发病机制和治疗方法时，科研人员利用脑立体定位仪将特定的神经毒素（如6 - 羟基多巴胺）精准注射到动物大脑的黑质等相关区域，模拟人类帕金森氏病的神经损伤过程，从而建立起帕金森氏病动物模型；

  
  

• 癫痫动物模型：癫痫是一种大脑神经元突发性异常放电，导致短暂的大脑功能障碍的慢性疾病。利用脑立体定位仪，将化学物质（如匹鲁卡品）或电刺激电极准确放置到动物大脑的特定脑区（如海马体），诱发动物产生癫痫发作，以此建立癫痫动物模型；

• 脑内肿瘤模型：在脑内肿瘤研究中，脑立体定位仪同样发挥着关键作用。科研人员可以将肿瘤细胞（如胶质瘤细胞）精确植入动物大脑的特定部位，构建脑内肿瘤模型。

• 脑内神经干细胞移植：神经干细胞移植是治疗神经系统疾病的一种潜在方法，脑立体定位仪在其中起到了关键的定位作用。科研人员可以通过脑立体定位仪将神经干细胞精确移植到大脑的损伤部位，期望神经干细胞能够分化为所需的神经元，修复受损的神经组织；

  
  

  例如，在脊髓损伤的研究中，利用脑立体定位仪将神经干细胞移植到损伤的脊髓部位，观察神经干细胞的存活、分化和对脊髓功能恢复的影响。相关研究为神经系统疾病的治疗带来了新的希望，许多研究成果发表在《Cell Stem Cell》等权威期刊上。

• 基因治疗研究：基因治疗是通过改变患者的基因来治疗疾病的一种新兴治疗方法。脑立体定位仪可用于将携带治疗基因的病毒载体（如腺相关病毒AAV）准确递送到大脑的特定区域 ，开展基因治疗研究。

1.Li, Qiuying et al. “Armcx1 Reduces Neurological Damage Via a Mitochondrial Transport Pathway Involving Miro1 After Traumatic Brain Injury.” Neuroscience vol. 545 (2024): 111-124. doi:10.1016/j.neuroscience.2024.03.009

2.Wang, Chaoyu et al. “Tetramethylpyrazine protects mitochondrial function by up-regulation of TFAM and inhibition of neuronal apoptosis in a rat model of surgical brain injury.” Iranian journal of basic medical sciences vol. 27,3 (2024): 352-359. doi:10.22038/IJBMS.2023.72947.15862

3.Duan, R., Hong, C. G., Chen, M. L., Wang, X., Pang, Z. L., Xie, H., & Liu, Z. Z. (2023). Targeting autophagy receptors OPTN and SQSTM1 as a novel therapeutic strategy for osteoporosis complicated with Alzheimer's disease. Chemico-Biological Interactions, 377, 110462.

4.Xia, Pengcheng et al. “MicroRNA-22-3p ameliorates Alzheimer's disease by targeting SOX9 through the NF-κB signaling pathway in the hippocampus.” Journal of neuroinflammation vol. 19,1 180. 12 Jul. 2022, doi:10.1186/s12974-022-02548-1

5.Chen, Meng-Lu et al. “Inhibition of miR-331-3p and miR-9-5p ameliorates Alzheimer's disease by enhancing autophagy.” Theranostics vol. 11,5 2395-2409. 1 Jan. 2021, doi:10.7150/thno.47408

6.Shen, Jie et al. “PDGFR-beta signaling mediates endogenous neurogenesis after postischemic neural stem/progenitor cell transplantation in mice.” Brain injury vol. 37,12-14 (2023): 1345-1354. doi:10.1080/02699052.2023.2280894

7.Wu, Muyao et al. “The Blood Component Iron Causes Neuronal Apoptosis Following Intracerebral Hemorrhage via the PERK Pathway.” Frontiers in neurology vol. 11 588548. 7 Dec. 2020, doi:10.3389/fneur.2020.588548

8.Jiang C, Wu X, Wang J, Li C, Luo G. Activation of CB1 pathway in the perirhinal cortex is necessary but not sufficient for destabilization of contextual fear memory in rats. Behavioural Brain Research. 2022;416:113573.

9.Liu Z, Li H, Hong C, et al. ALS-associated E478G mutation in human OPTN (Optineurin) promotes inflammation and induces neuronal cell death. Frontiers in immunology. 2018;9:2647.

10.Zhang Z, Chen W, Luan J, Chen D, Liu L, Feng X. Ameliorative effects of olibanum essential oil on learning and memory in Aβ1-42-induced Alzheimer’s disease mouse model. Tropical Journal of Pharmaceutical Research. 2020;19(8):1643-1651.

11.Wang Y, Zhang H, Hua L, et al. Curcumin prevents Alzheimer’s disease progression by upregulating JMJD3. American Journal of Translational Research. 2022;14(8):5280.

12.Shi M, Gong Y, Wu M, et al. Downregulation of TREM2/NF-кB signaling may damage the blood-brain barrier and aggravate neuronal apoptosis in experimental rats with surgically injured brain. Brain Research Bulletin. 2022/06/01/ 2022;183:116-126.

13.Zhang B-J, Yuan C-X. Effects of ADAM2 silencing on isoflurane-induced cognitive dysfunction via the P13K/Akt signaling pathway in immature rats. Biomedicine & Pharmacotherapy. 2019/01/01/ 2019;109:217-225.

14.Chen ML, Hong CG, Yue T, et al. Inhibition of miR-331-3p and miR-9-5p ameliorates Alzheimer's disease by enhancing autophagy. Theranostics. 2021;11(5):2395-2409.

15.Gong Y, Wu M, Shen J, et al. Inhibition of the NKCC1/NF-κB signaling pathway decreases inflammation and improves brain edema and nerve cell apoptosis in an SBI rat model. Frontiers in Molecular Neuroscience. 2021;14:641993.

16.Zhang D, Pan N, Jiang C, Hao M. LncRNA SNHG8 sponges miR-449c-5p and regulates the SIRT1/FoxO1 pathway to affect microglia activation and blood-brain barrier permeability in ischemic stroke. Journal of Leukocyte Biology. 2022/05/01 2022;111(5):953-966.

17.Zhou G, Ye Q, Xu Y, et al. Mitochondrial calcium uptake 3 mitigates cerebral amyloid angiopathy-related neuronal death and glial inflammation by reducing mitochondrial dysfunction. International Immunopharmacology. 2023/04/01/ 2023;117:109614.

18.Liu FX, Niu YG, Zhang DP, et al. Modified Protocol for Establishment of Intracranial Arterial Dolichoectasia Model by Injection of Elastase Into Cerebellomedullary Cistern in Mice. Frontiers in Neurology. 2022:748.

19.Xu Z, Lu W, Miao Y, Li H, Xie X, Zhang F. mRNA profiling reveals the potential mechanism of TIPE2 in attenuating cognitive deficits in APP/PS1 mice. International Immunopharmacology. 2020;87:106792.

20.Hu WB, Wang X, Pang ZL, Duan R, Hong CG, Liu ZZ. Neutralizing peripheral circulating IL1β slows the progression of ALS in a lentivirus‐infected OPTNE478G mouse model. Animal Models and Experimental Medicine. 2022.

21.Jiao, Yingfu et al. “Pre-treatment with morphine prevents lipopolysaccharide-induced acute respiratory distress syndrome in rats via activation of opioid receptors.” Experimental cell research vol. 418,1 (2022): 113224. doi:10.1016/j.yexcr.2022.113224

22.Liu, Yamei et al. “Rescuing Kv10.2 protein changes cognitive and emotional function in kainic acid-induced status epilepticus rats.” Epilepsy & behavior : E&B vol. 106 (2020): 106894. doi:10.1016/j.yebeh.2019.106894

23.Huang S, Hu W, Rao D, et al. RIPK3-Dependent Necroptosis Activates MCP-1-Mediated Inflammation in Mice after Intracerebral Hemorrhage. Journal of Stroke and Cerebrovascular Diseases. 2022;31(1):106213.