-
解决方案
-
运用高速逆流色谱法从仙茅分离仙茅甙和仙茅甙乙
发布:上海同田生物技术股份有限公司浏览次数:540Abstract: A preparative high-speed counter-current chromatography (HSCCC) was used to isolate and separate curculigoside and curculigoside B from the famous traditional Chinese medicinal herb Curculigo orchioides. Some parameters including two-phase solvent system, separation temperature,flow rate of the mobile phase and revolution speed of the apparatus were all investigated, and a successfully isolation and purification was achieved at the following conditions: the two-phase solvent system composed of ethyl acetate-ethanol-water at a volume ratio of 5:1:5(v/v/v) was selected and the lower phase of the system was used as the mobile phase at the flow rate of 2.0ml/min, and the isolation temperature and revolution speed were set at 30◦C and 800rpm.The isolation produced a total of 14.5 mg curculigoside B and 72.8 mg curculigoside with purities of 96.5% and 99.4% determined by high performance liquid chromatography (HPLC) from 300 mg crude extract after cleaning-up by D101 macroporous resin,which was necessary for the excellent purification. The recoveries of the two compounds were 91.6% and 92.5%, respectively, and the chemical structure identification was carried out by UV, MS and NMR. This separation method was more effective than some conventional techniques. TBE-300A高速逆流色谱仪/制备色谱仪 TBE300B+AKTA高速逆流色谱仪/离心分配色谱/萃取仪/制备色谱仪 TBE-1000A制备色谱仪/萃取仪/快速分离制备色谱仪 TBE-200V 高速逆流色谱仪 TBE-20A分析型高速逆流色谱仪/萃取仪/制备色谱仪
2007-02-10相关仪器 -
免责声明
①本网刊载上述内容,并不代表本网赞同其观点或证实其内容的真实性,不承担此类作品侵权行为的直接责任及连带责任
②若本站内容侵犯到您的合法权益,请及时告诉,我们马上修改或删除。邮箱:hezou_yiqi
-
认证会员 第 10 年
上海同田生物技术股份有限公司
认证:工商信息已核实
- 产品分类
- 品牌分类
-
仪企号上海同田生物技术股份有限公司
-
友情链接
-
手机版开启全新的世界m.yiqi.com/zt2944/