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解决方案
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运用高速逆流色谱从白术分离纯化苍术酮和苍术烯丙酯
发布:上海同田生物技术股份有限公司浏览次数:552The medicinal plant Atractylodes macrocephala (Baizhu in Chinese) has been widely used in traditional Chinese medicine for energy and stomach complaints, treatment of dyspepsia and anorexia, anti-inflammation, anticancer and for increasing assimilation. A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of two main bioactive components,namely, atractylon and atractylenolide III from A. macrocephala by using light petroleum (60908C)ethyl acetateethanolwater (4 :1:4:1 v/v) as the two-phase solvent system in dual-mode elution. Compared with the separation using the normal-mode elution, the dual-mode HSCCC can be achieved with shorter elution time.Atractylenolide III (32.1 mg) at 99.0% purity and 319.6 mg atractylon at 97.8% purity could be obtained from 1000 mg crude sample in a single run. The recoveries of atractylenolide III and atractylon were 95.4 and 92.6%, respectively. 高速逆流色谱仪/GX逆流色谱仪 TBE-1000A制备色谱仪/萃取仪/快速分离制备色谱仪 TBE300B+AKTA高速逆流色谱仪/离心分配色谱/萃取仪/制备色谱仪 TBE-200V 高速逆流色谱仪
2007-01-15相关仪器 -
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