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肝癌细胞中的循环生物标记物研究
发布:铂悦仪器(上海)有限公司浏览次数:1107Abstract Purpose Our aims are to determine levels of circulating cellular and protein biomarkers in hepatocellular carcinoma (HCC) patients and to analyse any relationships with clinical parameters. Methods Fifty-four consenting patients were recruited.Circulating tumour cells (CTCs) were enumerated (by Cell-Search) and characterised via filtration [by isolation by size of epithelial tumour cells (ISET)] with downstream immunohistochemistry (IHC). Glypican-3 (GPC3) expression in tumour biopsies and CTCs (by IHC) was compared, and levels of circulating caspase-cleaved and full-length cytokeratin 18 (CK18, measured using M30 and M65 ELISAs) were examined as a putative prognostic factor and marker of tumour burden. Results CTCs were identified in 14 out of 50 (28 %) patients by CellSearch and in 19 out of 19 (100 %) patients by ISET. The presence of GPC3-positive CTCs by ISET was 100 % concordant with the presence of GPC3-positive cells in the original tumour (n = 5). No statistically significant correlations were observed between CTC number and clinical characteristics, although trends were noted between CTC subtypes, ChildPugh score and tumour node metastasis stage. Serum M30 and M65 levels (as continuous variables) significantly correlated with overall survival (OS)in a univariate analysis (p = 0.003 and p < 0.001, respectively);M65 levels remained statistically significant in a multivariate analysis (p = 0.029). Conclusions This is the first study to detect GPC3-positive CTCs in HCC, important for drug development with this target. The significant association of circulating CK18 with OS in HCC further exemplifies the utility of circulating biomarkers in cancer. ISET循环肿瘤细胞捕获仪
2016-01-18上一篇:非小细胞肺癌患者的循环肿瘤细胞分析
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未认证会员 第 10 年
铂悦仪器(上海)有限公司
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- ISET循环肿瘤细胞捕获仪
- 循环肿瘤细胞检测cell search
- TXRF血液重金属检测
- 生物原子力显微镜
- 小动物活体成像Micro CT
- 超薄切片机
- 双光子荧光显微镜
- PCR和定量PCR
- 凝胶成像系统
- 电泳和转印系统
- 核酸纯化和检测
- 实验室常规设备
- 试剂盒
- 微生物现场快速检测仪
- 样品处理
- 自动化移液工作站
- Ultima活体多光子显微镜
- 超高分辨率显微镜Vutara350
- illumina测序仪
- 组织细胞毒性多光子断层扫描系统
- 流式细胞仪
- 纳米标尺
- 微区X射线荧光光谱仪
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