Abstract
Purpose Our aims are to determine levels of circulating cellular and protein biomarkers in hepatocellular carcinoma (HCC) patients and to analyse any relationships with clinical parameters.
Methods Fifty-four consenting patients were recruited.Circulating tumour cells (CTCs) were enumerated (by Cell-Search) and characterised via filtration [by isolation by size of epithelial tumour cells (ISET)] with downstream immunohistochemistry (IHC). Glypican-3 (GPC3) expression in tumour biopsies and CTCs (by IHC) was compared, and levels of circulating caspase-cleaved and full-length cytokeratin 18 (CK18, measured using M30 and M65 ELISAs) were examined as a putative prognostic factor and marker of tumour burden.
Results CTCs were identified in 14 out of 50 (28 %) patients by CellSearch and in 19 out of 19 (100 %) patients by ISET. The presence of GPC3-positive CTCs by ISET was 100 % concordant with the presence of GPC3-positive cells in the original tumour (n = 5). No statistically significant correlations were observed between CTC number and clinical characteristics, although trends were noted between CTC subtypes, ChildPugh score and tumour node metastasis stage. Serum M30 and M65 levels (as continuous variables) significantly correlated with overall survival (OS)in a univariate analysis (p = 0.003 and p < 0.001, respectively);M65 levels remained statistically significant in a multivariate analysis (p = 0.029).
Conclusions This is the first study to detect GPC3-positive CTCs in HCC, important for drug development with this target. The significant association of circulating CK18 with OS in HCC further exemplifies the utility of circulating biomarkers in cancer. ISET循环肿瘤细胞捕获仪
梅特勒-托利多,METTLER-TOLEDO(纽约证券交易所代码:MTD):世界上首台替代法单盘天平的fa明
Agilent GeneSpring and Mass Profiler Professional (MPP
The presence of hidden nut and tree nut allergens in f
This study investigated the feasibility of automating
SPEX 2010高通量动植物组织研磨机可以同时GX均质576个样品,一般样品的处理时间只需2分钟,大大提高
线粒体的蛋白质组分析有助提供深入的线粒体相关功能:老化,各种各样的代谢状况和疾病,如癌症,糖尿病和心血管疾病
1. 细胞培养 10cm细胞培养皿,50ml15ml一次性离心管,10ml玻璃吸管(灭菌),与玻璃吸管配套
4. 从液氮保存罐中取出冻存管,立即放入400C水浴中,快速摇晃,直至冻存液完全融化 5. 将细胞悬液移
以分析其表达,测定表达对细胞生长的影响,或将高表达的基因产物纯化将外源DNA导入哺乳动物细胞有两种途径:稳定