CCAAT增强子结合蛋白α（CEBPα）检测试剂盒

CCAAT增强子结合蛋白α（CEBPα）检测试剂盒 点击次数：36 发布时间：2015/5/7

CCAAT增强子结合蛋白α(CEBPα)检测试剂盒

适用生物 Homo sapiens (Human，人)
CCAAT增强子结合蛋白α(CEBPα)检测试剂盒检测范围 0.156-10ng/mL 灵敏度 0.055ng/mL
样本类型 Tissue homogenates, cell lysates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法 CCAAT增强子结合蛋白α(CEBPα)检测试剂盒规格 96T

ELISA Kit for CCAAT/Enhancer Binding Protein Alpha (CEBPa)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Specificity

This assay has high sensitivity and excellent specificity for detection of CCAAT/Enhancer Binding Protein Alpha (CEBPa).
No significant cross-reactivity or interference between CCAAT/Enhancer Binding Protein Alpha (CEBPa) and analogues was observed.

CCAAT增强子结合蛋白α(CEBPα)检测试剂盒Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CCAAT/Enhancer Binding Protein Alpha (CEBPa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CCAAT/Enhancer Binding Protein Alpha (CEBPa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature 首ld be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50μL Stop Solution. Read at 450nm immediately.

CCAAT增强子结合蛋白α(CEBPα)检测试剂盒Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCAAT/Enhancer Binding Protein Alpha (CEBPa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CCAAT/Enhancer Binding Protein Alpha (CEBPa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCAAT/Enhancer Binding Protein Alpha (CEBPa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CCAAT/Enhancer Binding Protein Alpha (CEBPa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.