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- 胞妹瞧材 2016-03-25 00:00:00
- 你好,“don't sleep here”翻译成中文是: 不要在这里睡觉。
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- 雅坤苑 2016-03-25 00:00:00
- don,t sleep here 不要在这里睡觉 sleep 英[sli:p] 美[slip] vi. 睡,睡觉; vi. 睡,睡觉; 睡眠状态; vt. 为…提供床位; 提供住宿; 以睡觉打发日子; [网络] 函数; 动词; 正常睡眠; [例句]Some children can sleep through any kind of noise. 有些孩子再怎么吵都照睡不误。 [其他] 第三人称单数:sleeps 现在分词:sleeping 过去式:slept过去分词:slept 双语例句 柯林斯词典 英英释义 百度百科 相关单词: Sleep [词典] [人名]斯利普 SLEEP abbr. scanning low energy electron probe 扫描低能电子探针
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热门问答
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2012-06-23 06:39:56
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- 片段翻译成中文
- The amplification was carried out on a PTC-200 thermocycler (MJ Research) in a 30m l reaction mixture containing10mM Tris–HCl (pH 8.3), 1.5mM MgCl2, 50mM KCl,150mM of each dNTP, 0.3mM of each primer (synthesized by TaKaRa), 1.0m l (about 1... The amplification was carried out on a PTC-200 thermocycler (MJ Research) in a 30m l reaction mixture containing10mM Tris–HCl (pH 8.3), 1.5mM MgCl2, 50mM KCl,150mM of each dNTP, 0.3mM of each primer (synthesized by TaKaRa), 1.0m l (about 100 ng) template DNA, and 1U Taq DNA polymerase (Promega). The reaction mixtures were denatured at 95 °C for 5 min and subjected to 30 cycles of 40 s at 95 °C, 1 min at 55 °C, 1.5 min at 72 °C, and a final extension step of 7 min at 72 °C. Amplified products were purified using WizardTM PCR Prep DNA Kit (Promega) according to the manufacturer’s instructions, then the two strands were sequenced directly with PRISMTM BigDye Terminator Ready Reaction Kit (Applied Biosystems) on an ABI 310 Genetic Analyzer (Perkin Elmer). 展开
2009-03-22 06:02:57
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- 生物化学翻译
- To show that HAX-1 degradation is part of the apoptotic process and any involvement Omi may have, we used the ucf-101 inhibitor. ucf-101 is a specific inhibitor of the proteolytic activity of Omi and has been described previously (13). When... To show that HAX-1 degradation is part of the apoptotic process and any involvement Omi may have, we used the ucf-101 inhibitor. ucf-101 is a specific inhibitor of the proteolytic activity of Omi and has been described previously (13). When HK-2 cells were treated with cisplatin in the presence of ucf-101, the percentage of apoptotic cells decreased and the inhibitor significantly blocked HAX-1 degradation. This effect was more pronounced when a higher concentration of the inhibitor was used. To confirm the specificity of the inhibitor in this system and exclude the possibility that another protease rather than Omi is involved in HAX-1 cleavage, we used cell lines derived from mnd2 mice (9). The parent cell line (mnd2-MSCV) derived from mouse embryo fibroblasts has no detectable Omi proteolytic activity (9). The same cell line has been transfected with wild type human Omi cDNA (mnd2-MSCV-Omi) and expresses high levels of active Omi protein (14). We found that in mnd2-MSCV cells, when induced to undergo apoptosis with various stimuli, the number of apoptotic cells was very low. Furthermore, no detectable cleavage of HAX-1 was observed. This is in contrast with the mnd2-MSCV-Omi cells where apoptosis was robust, and HAX-1 levels were inversely proportional to the degree of apoptosis. This experiment clearly shows that Omi is solely responsible for HAX-1 cleavage, which is essential for apoptosis under the conditions used in these experiments. HAX-1 subcellular localization depends on cell type (21, 30) and has been reported to be present in the mitochondria, cytoplasm, or plasma membrane (10, 21, 22, 30). We performed subcellular fractionation to investigate where HAX-1 cleavage by Omi takes place. We found that, in HEK293 cells, HAX-1 was predominantly present in the mitochondria, and this localization did not change in response to apoptotic stimuli. This suggests that Omi can initiate apoptosis in the mitochondria by cleaving HAX-1 protein. This is in accord with a recent study that shows Omi can induce apoptosis in human neutrophils treated with TNF- without being released from the mitochondria (7). Although several studies clearly define HAX-1 as an anti-apoptotic protein, the mechanism of its function is unknown. HAX-1 has sequence similarity to Bcl-2 family of proteins (10, 22). 展开
2007-02-03 21:19:55
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- 求翻译。。。。。。。。。。。
- During the preparation of the nano-products, these nano-units, such as nanoparticles, nanoclusters, nanowires and nanorods, can also self-assemble into the novel structural aggregates by several routes, including electron irradiation deposi... During the preparation of the nano-products, these nano-units, such as nanoparticles, nanoclusters, nanowires and nanorods, can also self-assemble into the novel structural aggregates by several routes, including electron irradiation deposition [19], chemical vapor deposition [20], laser vaporization-condensation [21], charge transferring [22], an organic reagent-assisted method [23], solution-liquid-solid method [24] and catalytic vapor-liquid-solid growth [25]. With these routes, various nanoscale or microscale aggregates can demonstrate novel architectures, including tree-like, web-like, spherical, nanowire-like, network and fishbone-like aggregates. As a well-known method for producing the nanocapsules, however, arc-discharge has been rarely used to synthesize the aggregates self-assembled by the nanocapsules prepared simultaneously in arc-discharge. Nevertheless, it is possible that the arc-discharge can be developed into a new way to synthesize the aggregates. In the present work, we utilized arc-discharge technique with modified strategies, involving changing the hydrogen pressure, introducing gadolinium - aluminum alloy ingot as the anode and adjusting the elements percent of the anode according to their evaporation pressure, to synthesize a new type of nanocapsules, with intermetallic compound GdAl2 as core and amorphous Al2O3 as shell, which enlarge the family of the magnetic nanocapsules. At the same time, the regularly aligned three-dimensional macro-aggregates self-assembled by the nanocapsules without any template and catalyst were simultaneously synthesized in arc-discharge process. 展开
2008-06-09 10:41:10
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