仪器网(yiqi.com)欢迎您!

| 注册 登录
网站首页-资讯-专题- 微头条-话题-产品- 品牌库-搜索-供应商- 展会-招标-采购- 社区-知识-技术-资料库-方案-产品库- 视频

资料库

仪器网> 资料库>半乳糖凝集素9蛋白galectin 9 peptide说明书

半乳糖凝集素9蛋白galectin 9 peptide说明书

本文由 上海恪敏生物科技有限公司 整理汇编

2018-10-01 10:01 1778阅读次数

扫    码    分   享
文档仅可预览首页内容,请下载后查看全文信息!
立即下载
DESCRIPTIONSourceE.coliderivedAla2Thr323Accession#BAA31542NterminalSequenceAnalysisAla2Structure/FormMonomerPredictedMolecularMass35.8kDaSPECIFICATIONSSDSPAGE34kDa,reducingconditionsActivityMeasuredbyitsabilitytoinduceapoptosisofJurkathumanacuteTcellleukemiacells.Lu,L.H.etal.(2007)J.Biochem.141:157.Theforthiseffectistypically15ug/mL.Measuredbyitsabilitytoagglutinatehumanredbloodcells.Hadari,Y.R.etal.(2000)J.CellSci.113:2385.Theforthiseffectistypically2.5-12.5μg/mL.EndotoxinLevel<1.0EUper1μgoftheproteinbytheLALmethod.Purity>95%,bySDSPAGEunderreducingconditionsandvisualizedbysilverstain.FormulationLyophilizedfroma0.2μmfilteredsolutioninMOPS,NaCl,EDTA,DTTandTrehalose.SeeCertificateofAnalysisfordetails.PREPARATIONANDSTORAGEReconstitutionReconstituteat100μg/mLinwater.ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.Stability&StorageUseamanualdefrostfreezerandavoidrepeatedfreezethawcycles.l12monthsfromdateofreceipt,20to70°Cassupplied.l1month,2to8°Cundersterileconditionsafterreconstitution.l3months,20to70°Cundersterileconditionsafterreconstitution.BACKGROUNDGalectinscompriseafamilyofmultifunctionalcarbohydratebindingproteinswithspecificityforN-acetyllactosaminecontainingglycoproteins.Atleast14mammalianGalectinssharestructuralsimilaritiesintheircarbohydraterecognitiondomains(CRD),formingthreegroups:prototype(oneCRD),tandemrepeat(twoCRDs),andchimeric(oneCRD,uniqueN-terminus)(1,2).FulllengthGalectin9isawidelyexpressed39kDatandemrepeatGalectinthatcontainstwoCRDsconnectedbyalinkerregion(3).Progressivedeletionwithinthelinkerregiongeneratesa36kDaisoform,alsoknownasEcalectinorUAT,aswellasa35kDaisoform(4).ThisrecombinantproteincorrespondstotheEcalectinisoformofhumanGalectin9andshares70%and73%aasequenceidentitywiththecorrespondingregionsofmouseandratGalectin9,respectively.Galectin9exhibitsawiderangeofactivities.Allthreeisoformsfunctionaseosinophilchemoattractants(5,6).Thisactivityisdestroyedbythrombinmediatedcleavagewithinthelinkerregionofthelongisoform,althoughtheEcalectinisoformisresistanttothrombin(7).Galectin9bindstocarbohydratemoietiesofIgE,therebypreventingimmunecomplexformation,mastcelldegranulation,andasthmaticandcutaneousanaphylaxisreactions(8).Independentofitslectinproperties,Galectin9inducesthematurationofdendriticcellswhichpromoteTh1polarization(9).Galectin9inducescellularapoptosisinpartbydirectbindingtoTIM3(10,11).ItsinteractionwithTIM3inhibitsTh1cellandCD8+cytotoxicTcellresponsesandalsopromotesregulatoryTcelldifferentiationandactivity(11,12).Galectin9suppressestumorcellmetastasisbyinterferingwiththeassociationsbetweenhyaluronicacidandCD44andbetweenVCAM1andIntegrinα4β1(13).TheEcalectinisoform(UATuratetransporter)canalsobeexpressedasanintegralmembraneproteinandmediatethecellulareffluxofurate(14).References:1.Yang,RY.etal.(2008)ExpertRev.Mol.Med.10:e17.2.Elola,M.T.etal.(2007)Cell.Mol.LifeSci.64:1679.3.Tureci,O.etal.(1997)J.Biol.Chem.272:6416.4.Chabot,S.etal.(2002)Glycobiology12:111.5.Matsumoto,R.etal.(2002)J.Immunol.168:1961.6.Sato,M.etal.(2002)Glycobiology12:191.7.Nishi,N.etal.(2006)Glycobiology16:15C.8.Niki,T.etal.(2009)J.Biol.Chem.284:32344.9.Dai,S.Y.etal.(2005)J.Immunol.175:2974.10.Seki,M.etal.(2007)ArthritisRheum.56:3968.11.Zhu,C.etal.(2005)Nat.Immunol.6:1245.12.Sehrawat,S.etal.(2010)PloSPathogens6:e1000882.13.Nobumoto,A.etal.(2008)Glycobiology18:735.14.LealPinto,E.etal.(2002)Am.J.Physiol.RenalPhysiol.283:F150.

参与评论

全部评论(0条)

获取验证码
我已经阅读并接受《仪器网服务协议》

更多资料

该会员其他资料
科博肽(α-神经毒素)
类凝血酶(降纤酶)
磷脂酶 A2
心脏毒素Cardiotoxin(质量标准)
眼镜蛇毒因子(CVF)(质量标准)
Rabbit Anti-Tau protein
Recombinant Human F3 / Tissue factor / CD142
(原装进口)人高分子量脂联素(HMW Adiponectin)Elisa试剂盒说明书
ANTI-HUMAN PAPILLOMA VIRUS 16,18 E6 (C1P5)
Anti-CTH/CSE/Cystathionase

在线留言

取消