无线吊秤C表英文说明书

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2018-09-30 10:01 941阅读次数

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• 无线吊秤C表英文说明书

  线数传式吊秤C型仪表产品特点:1,内置EPSON打印机,可以打印完整的称重数据.2,多达2900组称重数据存储,支持中文题头输入。3，完整的互换性，模块化的设计，维修变得更简单。4，全开放的程序，根据不同场合查以调整和设定5，可选配RS232串行输出，实现与上位机通讯。6，可选配4-20mA远程传输模块。无线数传式吊秤C型仪表[详细]

  2018-09-30 10:01

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• 无线吊秤XK3190-H2B仪表说明书

  一、无线吊秤称重仪表概述XK3190-H2B电气套件采用Σ－Δ模数变换技术，配上电子吊秤吊挂组件和外壳，即可构成功用完善的无线传输电子吊秤。套件包括：XK3190-H2B便携式称重显现器、H2AD型A/D模块、无线数传机、H2CDQ型智能疾速充电机、镉镍电池组及其它附件和有些消耗品。二、无线吊秤称重仪表技术参数A/D变换方法:Σ-Δ变换方法A/D变换速率:6次/秒ZdaA/D变换数:20bit输入信号规模:－2mV～28mV输入灵敏度:≥1μV/e传感器供桥电源:DC5V；I≤50mA；衔接传感器个数:可接4个350Ω的传感器传感器衔接方法:4线制检定分度数:3000Zda外分度数:30,000Zda内分辨率:300,000分度值:1/2/5/10/20/50可选显现方法:8位LCD数码管，19个状况指示符显现规模:-30000～30000三、无线吊秤称重仪表功用特点高精度A/D变换，可读性达1/30000；调用内码显现便利，代替感量砝码调查及剖析允差；键盘标定、抛物线法非线性修正，校准便利；具有负秤功用，可用于铸造等进程；置零（开机/手动）规模可别离设置；储存11个种类各99次称重记载；（称重时间、毛重或毛重等）称重记载储存断电维护；随机配置12V/1.6AH镍氢电池组供电；（外表供电）秤体用12V2200mAh镍镉电池组供电，可接连作业24小时以上；带5种维护模式的智能疾速充电器，有用延伸充电电池寿数；装备RS-232/（RS-422/485选配）通讯口，波特率可选，通讯方法可选；装备大屏幕通讯口，20mA电流环衔接方法；装备规范并行打印接口，可衔接24针宽行打印机；内置16列面板式微型打印机、打印称重清单；内部备有拓展接口，可按用户特殊要求拓展功用；[详细]

  2018-10-30 10:00

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• 大鼠C反应蛋白试剂盒英文说明书

  　　　大鼠C反应蛋白试剂盒英文说明书Assayrange：80μg/L-2400μg/L96determinationsPurposeThiskitallowsforthedeterminationofCRPconcentrationsinRatserum,cellculturesupernatesandotherbiologicalfluidsPrincipleoftheassayThekitassayRatCRPlevelinthesample，usePurifiedRatCRPantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddCRPtowells,CombinedCRPantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofRatCRPinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.Materialsprovidedwiththekit1washsolution20ml×1bottle7StoppSolution6ml×1bottle2HRP-Conjugatereagent6ml×1bottle8Standard（4800μg/L）0.5ml×1bottle3Microelisastripplate12well×8strips9Standarddiluent1.5ml×1bottle4Samplediluent6ml×1bottle10Instruction15ChromogenSolutionA6ml×1bottle11Closureplatemembrane26ChromogenSolutionB6ml×1bottle12Sealedbags1Specimenrequirements1.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.2.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.Assayprocedure1.Diluteandaddsample:DiluteOriginaldensityStandardasfollowtable:2400μg/L5Standard150μlOriginaldensityStandard+150μlStandarddiluent1200μg/L4Standard150μl5Standard+150μlStandarddiluent600μg/L3Standard150μl4Standard+150μlStandarddiluent300μg/L2Standard150μl3Standard+150μlStandarddiluent150μg/L1Standard150μl2Standard+150μlStandarddiluent2.addsample：Setblankwellsseparately（blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame）.testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl（samplefinaldilutionis5-fold）,addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-fold（or20-fold）washsolutiondiluted30-fold（or20-fold）withdistilledwaterandreserve.5.washing：UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.addenzyme：AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.incubate：Operationwith3.8.washing：Operationwith5.9.color：AddChromogenSolutionA50ulandChromogenSolutionBtoeachwell,evadethelightpreservationfor15minat37℃10.Stopthereaction：AddStopSolution50μltoeachwell,Stopthereaction（thebluecolorchangetoyellowcolor）.11.assay：takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.StepsdescriptionStandard,SamplediluentAddStandard,Samplediluent,incubatefor30minat37℃.Wash5time,AddHRP-Conjugatereagent,incubatefor30minat37℃.Wash5times,AddChromogenSolutionAandB,incubatefor30minat37℃.AddStoppSolutionReadabsorbanceat450nmwithin15mincalculateCalculateTakethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.Importantnotes1.Thekittakesoutfromtherefrigerationenvironment首ldbebalanced15-30minutesintheroomtemperature,ELISAplatescoatedifhasnotuseupafteropened,theplate首ldbestoredinSealedbag.2.washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.3.addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.4.ifthetestingmaterialcontentisexcessivelyhigher（ThesampleODisbiggerthanthefirststandardwell）,pleasediluteSample（n-fold）,Pleasediluenteandmultipliedbythedilutionfactor.（×n×5）.5.Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.6.Thesubstrateevadethelightpreservation.7.Pleaseaccordingtouseinstructionstrictly,Thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.8.Allsamples,washingbufferandeachkindofreject首ldaccordingtoinfectivematerialprocess.9.Donotmixreagentswiththosefromotherlots.Storageandvalidity1．Storage：2-8℃.2．validity：sixmonths[详细]

  2018-11-16 10:02

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• OCS-D4无线吊秤通信协议

  1．产品介绍无线数传电子吊秤是显示器和感应体分离的一种悬挂式电子秤，其目的是为了避免被称重物体积过大，挡住了操作人员的视线而不能极ng确读数而设计的，传输距离远、且不受附近其它波段影响；这样就可以避免由于客观原因造成的经济损失；2．功能特点显示仪表和感应体的间隔距离远，传输距离在小于等于200米；带微型16列针式打印机，可打印序号、日期、时间、车号、货号、净重、毛重、月报表、日报表、累计打印等功能；打印机和感应体都带12V蓄电池，充电一次可连续使用120小时以上；为感应体安装了备用电池，可以随时更换，避免了因感应体没电而无法工作的局面；3．量程3T、5T、10T、20、30T、50T4．误差0.5kg、1kg、2kg、5kg、10kg、20kg、50kg……500kg；5．其它机型：耐高温电子吊秤、直示电子吊秤；[详细]

  2018-10-06 10:00

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• （离心机）英文说明书

  BriefintroductionandapplicationscopeAscentrifugalseparator,thiselectriccentrifugalmachinemakesuseofthecentrifugalforcetoseparateeverycompositioninsolution,adoptsteplessspeedvariationandautomaticbalancedevices,sothismachineownsvariousadvantages,suchasstableperformance,smallvolume,beautifulappearance,lowertemperaturerising,highworkingefficiencyandwiderapplication,speciallyforitsaluminumalloycentrifugalrotary-disc,itcanguaranteetoprotectthecentrifugaltubesfromdamageeffectively.Thus,thiscentrifugalseparatorhasbeenwidelyusedinhospitalandchemicalorbiologylabtocarryoutqualitativeanalysisforbloodserum,bloodplasma,etc.Technicalparameters:Model800800D80-2TDL-50XYJ-3XYJ-4TDL-5Max.rev4000rpm5000rpmRCFMac1430（xg）1790（xg）5000（xg）Max.capacity20mlx6（Angietype）20mlx1220mlx810mlx16；50mlx8100mlx4Motorpower25W40W280WTimingrangeNo0-60min0-120minAutobalanceNoYesYesSelf-lockerNoNoNoNoNoYesWeight67121035ImportantNotice：●Foryoursafety,areliablegroundingisstronglyrecommendedtobedonebeforeoperation,●Beforethefirsttimeuse,pleasereadthisOperationManualcarefully.●Workingconditions:0-40℃ambienttemperatureand80%belowrelativehumidity.●Itissuggestedtocutoffpowerwhenlongtermnonuse.OperationProcedure:●Inserttheplugintosocketandturnonthepowerswitch.●Putthemedicineintocentrifugetubeandtheninsertthetubeintothecorrespondingsleeve,closethecoverplateandselectpropercentrifugalspeedaccordingtotestrequirements,thenselectrunningtimeorcloseON.Soitiseasytooperatethisinstrumentwithbettereffect.Dailymaintenance1.Correctoperationandmaintenancecanextendtheservicelife,itissuggestedtoplacethisinstrumentunderreasonableworkingconditionssothattheinstrumentcanworknormally.2.Iftheliquidissplashedorsprayedintoinstrumentbyaccident,cutoffthepowersupplyandremovetheresidualliquidincaseshortentheservicelife.Oneyearwarrantyandlifelongmaintenanceareofferedtoourproduct（reasonablelaborandmaterialcostwillbetriggeredbyman-madedamageormisoperation）[详细]

  2018-08-21 10:00

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• 2406N英文说明书

  由深圳市朗普电子科技有限公司代理深圳市深南中路南光捷佳大厦1402室TEL:0755-88851600（直线）0755-839801580755-830474150755-83986300FAX:0755-888505150755-83047419网址：综合推广网www.17lp.com衡器烘箱网www.1718sz.com邮箱：lp@df17.comQQ:　374542908MSN：dafusz@hotmail.com[详细]

  2018-09-19 10:01

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• 7725I英文说明书

  7725I英文说明书[详细]

  2024-09-19 04:04

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• SLPe使用说明书（英文）

  SLPe使用说明书（英文）[详细]

  2012-07-09 00:00

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• XK3190-A12E仪表英文说明书

  XK3190-A12E仪表校正操作方法如下：1、在校正电子地磅时应清空秤体台面2、要检查电子地磅的四角角差是否在允许范围内。3、打开XK3190-A12E称重显示仪表，开机过程中按住井号键不放，显示一串9后放手4、按一次功能键，进入零位校正，如秤台上无物品，四角差在范围内则按一次井号键进入砝码校正。5、放上砝码（大于1/3满量程），通过去皮键（用于移动位置）与置零键（0－9数字键）输入砝码值后稍等10几秒后按井号键显示END再按一次仪表后面的标定开关一次，校正完毕，再重新放一个砝码测试一下，如有数据有差别，那就要重新要检查一下电子地磅是否还存角差问题。再另重新标定校正一次。如称重数据等同砝码重量，则客户即可使用。具体操作方法示意图：[详细]

  2018-09-26 10:00

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• 孔雀石绿英文说明书.pdf

  malachitegreen（MG）ELISA1UsepurposeThiskitforFeed,aquaticsamples,watersamplesintheMGremainingquantitativedetection.2ExperimentalprincipleThiskitadoptsmethodsincompetitionELISAInmicroplatescoatedwithantigenconjugatedMG,addMGstandardorsamples,FreeMGandpre-coatedonstripsofMGconjugatedantigencompeteagainstMGantibodyconjugates,WithTMBchromogenicsubstrate,thecolorischangedfrombluetoyellowafteraddingstopsolution,enzymestandardinstrument450nmwavelengthsintesting,absorblightvalueandinthesamplewasMGcontentisinverselyproportionaltothestandardcurve,throughcalculationsampleswasMGconcentrations.3Materialsprovidedwiththekit3.1Microelisastripplate:1block（12well×8strips）.3.2MGstandard:sixvialsof（1ml/vial）,contentisrespectively:0PPB,0.1PPB,0.3PPBand0.9PPB,2.7PPB,8.1PPB.3.3Anti-MGantibodyconjugate:1vial（6ml）.3.4ChromogenSolutionA:1vial（6ml）.3.5ChromogenSolutionB:1vial（6ml）.3.6StopSolution:1vial（6ml）,2Msulphuricacid.3.7sampledilution:1vial（10x,6ml）,usedforsampledilutedwith.3.8washsolution:1vial（20x,20ml）,usedforwashingboard.3.9Instruction.4Neednotprovidematerials4.1equipment4.4.1wavelength450nmmicroplate.4.1.2shredder.4.1.3LiangTong.4.1.4oscillators.4.1.5funnel.4.1.6WhatmanNo1orequivalentfilterpaper.4.1.7traceremoveliquiddevice.4.2reagent4.2.1thedeionizedwaterordistilledwater.4.2.2methanol.5Storage5.1kitstoredin2~8℃,Don’tfrozen5.2Don’tuseuptheMicroelisastripplate首ldbesealeddryingpreserve6.Precautions6.1pleasereadtheinstructionscarefully,beforeuseingthekit.6.2don'tuseexpiredkit.6.3beforeusingthekit,pleaseletthereagentrecovertoroomtemperature（25+2℃）,theproposalforatleast2hourstotemperature.6.4thestandardcontainMG,payspecialattentionto,theoperation,we首ldbringgloves.6.5stopsolutioniscontainingsulfate,whenusing,preventburnsskinandcorrosionclothing.6.6differentstandardandsamplesuctionheadusedcannotbemixeduse,otherwise,itwillaffecttestresults.6.7differentbatchesofreagentkitnotmix,Differentstandardandsamplesuctionheadshallnotbeusedincombination,otherwise,itwillaffecttheexperimentalresult.6.8dilutedsamplemustusethiskitofsamplediluent,otherwise,itwillaffecttheexperimentalresult6.9mixedreagents首ldavoidblistering.7Workingliquidpreparation7.1Carbendazimstandards:0ppb,0.1PPB,0.3PPBand0.9PPB,2.7PPB,8.1PPB7.2washsolution:1:20withdistilledwater（1+19）diluted.preparation7.3sampledilutions:1:10withdistilledwater（1+9）diluted.preparation7.3ChromogenSolutionreagent:alreadystandby,avoidlightstraightas7.4stopsolution:alreadyterminationaside8Sampleprocessingprogram（sampleinextractionprocess,muststrictlyaccordingtotheoperationoftheextractionprocess首ldbeaccuratedilution,canappearotherwiseresultsareinaccurate,samples首ldbekeptinacoolplacetoavoidlightandfrozenkeep）8.1Smashthesamplestaken10g,add20ml70%methanolsolution8.2powerfuloscillation3minutes8.3WhatmanNo1filterwith8.4Takethe25μltreatmentofthesamplebyadding25μlsampledilutioninthewells（sampledilutionfactorof2）9Enzyme2lindedanalysissteps9.1experimentalguidelines9.1.1experimentbeginspriortoallreagentinboxesoutsidetheroomtemperature（25fullyrecoveredto+2℃）,timeabout2hours.Returntoroomtemperature（25+2℃）againafterremoveStrips,excessporebartosealimmediatelytothe2~8℃dryingpreserveNote:besuretotemperature,otherwisefullyguaranteetheaccuracyandprecisionoftheaffectingdetection.9.1.2afterusepleaseimmediatelyreagentputback2~8℃preservation9.1.3pleasedon'tchangeanalysisprogram9.1.4pleaseuseaccuratetraceremoveliquiddevice9.1.5operationoncestarted,pleasedonotinterruptanyprogram9.1.6ELISAresultsofrepeatabilityofseveredependsonoperatingprocedures,pleasestrictlyaccordingtorequirementsoperation9.1.7toavoidcross-contamination,eachstandardandsamples首ldusedifferentsuctionheadaddsamples9.1.8plussampledomakesuckaheadcontactmicroporousthesolutionorinsidesurface9.2analysissteps9.2.1beforehandnumbered,markB0,standardandthesamplepositionrecommendeddoubleorificedetection9.2.2taketherequiredamountoftheStrips（Stripsdetachable）,willspareattribwattleandsealedimmediatelyputbackagain2~8℃preservation9.2.3sampledilutions（10）,washsolutionx（20x）dilutionintoworkingliquid（distilledwaterordeionizedwaterdilute）In9.2.4B0welljoining50μl0.0ng/mlstandard9.2.5ineachstandardwelljoining50μlstandard9.2.6ineachsamplewelljoining50μlsamplesolution9.2.7Inallwelljoining50μlAnti-MGantibodyconjugate9.2.8gentlysloshingresponseboardforafewseconds.9.337℃warmbath30min（warmbathprocesssometimespatreactionplate,canreducedoubleorificeerror）9.3.1UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat..9.4reaction9.4.1washingprocedurecompleted,immediatelywithliquidapparatusineverytracemovemicroporousfirstjoin50μlChromogenSolutionA,add50μlChromogenSolutionB,Slightsloshingresponseboardmakethoroughlyincorporated379.4.2℃warmbath10min9.4.3eachwelljoining50μlStopSolution,blending9.4.4in450nmtestingabsorbency,resultin5mininsideread.10Theresultscalculated10.1quantitativeanalysis10.1.1obtainedbyeachconcentrationstandardsolutionandtheaveragevalueofasamplespectrophotometry（B）dividedbythefirststandard（0standardabsorbencyvalue（B0）multiplyingby1**%,namelypercentageabsorbencyvalues.B-standardsolutionorsamplesolutionofaverageabsorbanceofthevaluesB0-0μg/Lstandardsolutionofaverageabsorbanceofthevalues10.1.2withMGconcentrationsofvaluesfortheXaxis,100centabsorbanceofthevalueoftheYaxis,drawstandardcurve.Accordingtothesamplepercentageabsorbencyvalues,whichgetcorrespondingpointsfromcurve,namelytheabscissadenotesthemulti-goalMGconcentrationonthenumericalcurvature.theagainstseveralnamelytodetermineMGconcentrationC（ppb）10.1.3becausethesampleafterdilutedinadvance,soaccordingtothestandardcurvegainsfromdifferentconcentrationsamplesmustagainmultiplythedilutedtimes.10.2halfquantitatively10.1.1visualhalfquantitativedetermination:first,chooseanappropriatestandardfluidandsampleswithoperation,accordingtothesamplesandstandardsubstanceabsorbencyvaluethediscretionofthejudgeiscompared,samplechromavalueislessthanorgreaterthanstandardvalues.10.1.2instrumenthalfquantitativedetermination:first,chooseanappropriatestandardfluidandsampleswithoperation,accordingtothesampleandstandardcolordepthcomparison,judgesamplechromavalueislessthanorgreaterthanstandardvalues.11SpecificityPhysicalcrossreactionMG1**%CrystalViolet:95%Recessivemalachitegreen:0.1%HiddenCrystalViolet:<0.1%12KitparametersThiskitdetectionlimitis0.05PPBB0absorbanceoftheoptimalvalue首ldbegreaterthan1.0Kitabsorbencyboardinsideerrorislessthan8%,boardbetweenerrorislessthan15%.Withthismanualprovidedatissuesampleextractionmethodrecoveryisgreaterthan80%.13AnalysisrestrictionThiskittestingpositiveforsamples首lduseanothermethodsuchasHPLCorGC/MStobeverified.上海恒远生物科技有限公司，专业销售“孔雀石绿试剂盒”品质保证，值得信赖！如果你想了解该产品的详细信息，欢迎来电来函！[详细]

  2018-11-15 10:03

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• 电解测厚仪英文说明书

  主要针对一些院校外国留学生，对中文还不太懂的学生和出售到国外而准备的。[详细]

  2018-12-10 15:26

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• PZ表说明书

  该表采用交流采样技术，能分别 测量电网中的电流电压公路因数等技术参数[详细]

  2024-09-28 00:01

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• pPICZα C载体说明书

  pPICZαCOX1promoterregion:bases1-941OX1primingsite:bases855-875α-factorsignalsequence:bases941-1207Multiplecloningsite:bases1208-1276c-mycepitope:bases1275-1304Polyhistidine（6xHis）tag:bases1320-13373AOX1primingsite:bases1423-1443AOX1transcriptionterminationregion:bases1341-1682TEF1promoter:bases1683-2093EM7promoter:bases2095-2162ShbleORF:bases2163-2537CYC1transcriptionterminationregion:bases2538-2855pUCorigin:bases2866-3539（complementarystrand）质粒图谱:上海起福生物科技有限公司联系人：杨建辉400电话：4006551678办公电话：021-50724187传真：021-50724961*8006手机：15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址：上海市浦东新区绣川路561号1102室[详细]

  2018-09-29 10:02

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• pGAPZα C载体说明书

  pGAPZαC型号载体名称出品公司载体用途VJI0295pGAPZαCInvitrogen酵母表达载体Theglyceraldehyde-3-phosphatedehydrogenase（GAPDH）enzymeisconstitutivelyexpressedathighlevelsinmanyorganisms,includingPichiapastoris.Thepromoterofthegene（GAP）encodingtheGAPDHproteinhasrecentlybeencharacterizedandshowntoexpressrecombinantproteinstohighlevelsinPichiapastoris,dependingonthecarbonsourceused（Waterhametal.,1997）.ThelevelofexpressionseenwiththeGAPpromoter（PGAP）canbeslightlyhigherthanthatobtainedwiththeAOX1promoter.ThepGAPZA,B,andCvectors（2.9kb）andpGAPZαA,B,andC（3.1kb）vectorsusetheGAPpromotertoconstitutivelyexpressrecombinantproteinsinPichiapastoris.ProteinscanbeexpressedasfusionstoaC-terminalpeptidecontainingthemycepitopefordetectionandapolyhistidinetagforpurificationonmetal-chelatingresin（i.e.ProBond）.Inaddition,pGAPZαproducesproteinsfusedtoanN-terminalpeptideencodingtheSaccharomycescerevisiaeα-factorsecretionsignal.BothvectorsaresuppliedinthreereadingframestofacilitateinframecloningwiththeC-terminaltagand/ortheN-terminalsecretionsignal.Selectionofthesevectorsisbasedonthedominantselectablemarker,Zeocin,whichisbifunctionalinbothPichiaandE.coli.质粒图谱:上海起福生物科技有限公司联系人：杨建辉400电话：4006551678办公电话：021-50724187传真：021-50724961*8006手机：15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址：上海市浦东新区绣川路561号1102室[详细]

  2018-09-29 10:02

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• 多粘菌素C（Polymyxin-C）说明书

  1多粘菌素C（Polymyxin-C）酶联免疫分析（ELISA）试剂盒使用说明书本试剂盒仅供研究使用。1使用目的：本试剂盒用于肉类组织（如鸡、牛肉和猪肉）中多粘菌素C（Polymyxin-C）残留的定量检测。2实验原理本试剂盒采用竞争ELISA方法，在微孔板包被有多粘菌素C（Polymyxin-C）偶联抗原，加入多粘菌素C（Polymyxin-C）标准品或样品，游离多粘菌素C（Polymyxin-C）与微孔条上预包被的多粘菌素C（Polymyxin-C）偶联抗原互相竞争抗多粘菌素C（Polymyxin-C）抗体酶标记物，用TMB底物显色，加入终止液后颜色由蓝色变为黄色，用酶标仪在450nm波长下进行检测，吸光值与样品中多粘菌素C（Polymyxin-C）含量成反比，通过标准曲线计算样品中多粘菌素C（Polymyxin-C）的含量。3试剂盒组成3.1预包被的多粘菌素C（Polymyxin-C）偶联抗原的可拆酶标板：1块（12孔×8条）。3.2多粘菌素C（Polymyxin-C）标准品：6瓶（1ml/瓶），含量分别是：0ppb，0.1ppb,0.3ppb,0.9ppb,2.7ppb,8.1ppb。3.3抗多粘菌素C（Polymyxin-C）抗体酶结合物：1瓶（6ml）。3.4显色液A：1瓶（6ml）。3.5显色液B：1瓶（6ml）。3.6终止液：1瓶（6ml），2M硫酸。3.7样本稀释液：1瓶（10×,6ml），用于样品稀释用。3.8浓缩洗涤液：1瓶（20×，20ml），用于洗板。3.9说明书一份。4需要而未提供的材料4.1设备4.1.1波长450nm酶标仪。4.1.2粉碎机。4.1.3量筒。4.1.4振荡器。4.1.5漏斗。4.1.6WhatmanNo1或相当的滤纸。4.1.7微量移液器。4.2试剂4.2.1去离子水或蒸馏水。4.2.2甲醇。5贮存5.1试剂盒贮存于2~8℃，切勿冷冻5.2未用完的微孔板应该密封干燥保存6注意事项26.1使用试剂盒前请仔细阅读说明书。6.2不要使用过期试剂盒。6.3试剂盒使用前，将试剂恢复至室温（25±2℃），建议至少回温2小时。6.4标准品中含有多粘菌素C（Polymyxin-C），使用时应特别注意，操作时应带手套。6.5终止液中含有硫酸，使用时防止灼伤皮肤及腐蚀衣物。6.6不同标准品、样品所用吸头不能混用，否则会影响试验结果。6.7不同批号试剂盒中的试剂不得混用；不同标准品、样品所用吸头不得混用，否则会影响实验结果。6.8稀释样本时必须用本试剂盒中的样本稀释液，否则会影响实验结果6.9混合试剂时应避免起泡。7工作液准备7.1多粘菌素C（Polymyxin-C）标准品溶液：0ppb,0.1ppb,0.3ppb,0.9ppb,2.7ppb,8.1ppb7.2浓缩洗涤液：用蒸馏水按1:20（1+19）稀释备用7.3样本稀释液：用蒸馏水按1:10（1+9）稀释备用7.3显色剂：已备用，避免光线直照7.4反应终止液：已备用8样品处理程序（样品在提取过程中，要严格按说明书操作，提取过程中应准确稀释，否则会出现结果不准确，样品应当保存在阴凉避光之处及冷藏保存）8.1取10g粉碎的样品，加20ml70%甲醇溶液8.2QL振荡3分钟8.3用WhatmanNo1滤纸过滤8.4取100l处理后的样品，加入400l样本稀释液8.5取25l处理后的样品，加入25l样本稀释液于反应孔中（样本稀释倍数为2）9酶免分析步骤9.1实验须知9.1.1实验开始前请将所有试剂于盒外充分恢复至室温（25±2℃），时间约2小时。回温至室温（25±2℃）后再取出微孔条，多余的微孔条重新密封立即于2~8℃干燥保存注：一定保证回温充分，否则影响检测的极ng确度和准确度。9.1.2使用后请立即将试剂放回2~8℃保存9.1.3请不要改变分析程序9.1.4请使用极ng确的微量移液器9.1.5操作一旦开始，请不要中断任何程序9.1.6ELISA结果的可重复性极大程度的取决于操作程序，请严格按照要求操作9.1.7为避免交叉污染，每个标准品和样品均应使用不同的吸头加样9.1.8加样时请勿让吸头接触微孔中的溶液或内表面9.2分析步骤9.2.1预先进行编号，标记B0、标准品和样品的位置，推荐进行双孔检测9.2.2取所需数量的微孔（微孔条可拆），将多余板条重新密封并立即放回2~8℃保存9.2.3样品稀释液（10×）、浓缩洗涤液（20×）稀释成工作液（蒸馏水或去离子水稀释）9.2.4在B0孔中加入50l0.0ng/ml标准品溶液9.2.5在各标准孔中加入50l的标准品溶液9.2.6在各样品孔中加入50l样品溶液9.2.7在所有孔中加入50l的抗多粘菌素C（Polymyxin-C）抗体酶结合物9.2.8轻轻晃动反应板几秒钟。39.337℃温浴30min（温浴过程中不时轻拍反应板，可以减少双孔误差）9.3.1甩掉孔中液体，用洗液洗涤微孔板5次，Z后一次应在吸水纸上拍打以完全除去孔中液体。9.4反应9.4.1洗涤程序完成后，立即用微量移液器在每个微孔中先加入50l显色液A，再加50l显色液B；轻微晃动反应板使之彻底混匀9.4.237℃温浴10min9.4.3每孔中加入50l终止液，混匀9.4.4在450nm下检测吸光度，结果在5min内读取。10结果计算10.1定量分析10.1.1所获得的每个浓度标准溶液和样本吸光度值的平均值（B）除以**个标准（0标准）的吸光度值（B0）再乘以1**%，即百分吸光度值。B标准溶液或样本溶液的平均吸光度值B00g/L标准溶液的平均吸光度值10.1.2以多粘菌素C（Polymyxin-C）浓度的对数值为X轴，百分吸光度值为Y轴，绘制标准曲线图。根据样品百分吸光度值，可从曲线上得到对应点的横坐标，即为多粘菌素C（Polymyxin-C）浓度的对数值，求得反对数即为测定液中多粘菌素C（Polymyxin-C）浓度C（ppb）10.1.3由于样品经过了预先稀释，因此根据标准曲线所得出的样品浓度一定要再乘以其稀释倍数。10.2半定量测定10.1.1目测半定量测定：首先选择一个适当的标准液与样品同运行，根据样品与标准品吸光度值的高低比较，判断样品浓度值是小于还是大于标准值。10.1.2仪器半定量测定：首先选择一个适当的标准液与样品同运行，根据样品与标准品颜色深浅比较，判断样品浓度值是小于还是大于标准值。11特异性物质交叉反应多粘菌素C（Polymyxin-C）1**%多粘菌素A……………………＜0.1%多粘菌素B……………………＜0.1%多粘菌素D……………………＜0.2%多粘菌素E……………………＜0.1%12试剂盒参数本试剂盒检测下限为0.05ppbB0吸光度**值应大于1.0试剂盒吸光度板内误差小于8％，板间误差小于15％。用本说明书提供的组织样本提取方法回收率大于80％。13标准曲线模式（仅供参考）试剂盒提供的标准曲线范围为0.1ppb~8.1ppb。414分析限制本试剂盒检测为阳性的样品应该用另一种方法如HPLC或GC/MS加以确证。[详细]

  2018-11-15 10:03

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• 标准三角带 A,B,C,D,Z尺寸表

  三角带有特种带芯结构和绳芯结构两种，分别由包布、顶胶、抗拉体和底胶四部分组成。绳芯结构的三角带制造方便，抗拉强度一般，价格低廉，应用广泛特种带芯结构的三角带韧性好，强度高，适用于转速较高的场合。*顶宽:13mm*Z小轮径:75mm*高度:8mm*Li（内长）=La（外长）-50mm*角度:40°*Li（内长）=Lp（节园长）-36mm型号Limm型号Limm型号Limm型号LimmA15381A421067A721829A962438A18457A431092A741880A982489A20508A431/2110751909925122559A441118A761930A10125623584A451143A771956A1022591A231/2597A451/21156A781727A1032616A24610A461168A791753A1042642A2563461/21181A801778A1052667A26660A471194A811803A1072718A27686A481219A831854A1082743A28711A49124851901102794A29737A501270A861930A112284291/2749A51129871956A1132870A30762A521321A881981A1142896A31787A531346A892007A1152921A32813A551397A912057A1182997A321/2826A561422A922083A1203048A33838A571448A832108A1243150A34864A581473A831/22121A125317341/2876A591499A842134A1263200A35889A601524A841/22146A1283251A351/2902A611549A852159A1303302A36914A62157862184A1323353A37940A631600A872210A1403556A3896651651A892261A1483759A381/2978A661676A902286A1543912A39991A671702A912311A162411401016A681727A922337A1674242A411041A701778A942388A1924877A411/21054A711803A952413A1975004[详细]

  2024-10-03 03:36

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• FP640火焰光度计英文使用说明书

  FP640火焰光度计英文使用说明书[详细]

  2014-08-08 00:00

  标准

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• FP6410火焰光度计英文使用说明书

  FP6410火焰光度计英文使用说明书[详细]

  2014-08-08 00:00

  操作手册

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• XK3190-C8控制仪表英文说明书

  XK3190-C8控制仪表英文说明书XK3190-C8仪表采用高速的Σ-△模数转换技术。适用于电子配料秤、定值秤、分选秤等控制场合，同时具有上下限报警、峰值保持功能。3路光隔离开关量输入，3路开关量（内置3路继电器）输出控制高精度A/D转换，可读性达1/300005点非线性修正换表无需重新标定，只需输入原仪表的参数即可使用集加/减法秤/分选秤/上下限报警/峰值保持等功能于一体定量值、提前量、上下限、节点延时等参数可根据应用自由设置加/减法秤模式下可选1-2种配料，分选秤模式下可选外控/自检/自由上下限模式准确度等级：3级，nind=3000AD转换方式：Σ-△转换方式AD转换码：24bit转换速度：80次/秒输入信号范围：-19mV～+19mV输入灵敏度：≥1uV/e非线性：≤0.01%F.S满量程温度系数：≤8PPM/℃传感器供桥电源：DC：5V；350mA传感器连接个数：1~8[详细]

  2018-10-07 10:04

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• HC316压力变送器英文使用说明书

  HC316系列压力变送器采用进口扩散硅或陶瓷芯体作为压力检测元件，传感器信号经高性能电子放大器转换成0~ 10mA或4~ 20mA统一输出信号。可替代传统的远传压力表、霍尔元件、差动变送器。能与各种型号的动圈式指示仪、数字压力表、电子电位差计配套使用，也能与各种自动调节系统或计算机系统配套使用。广泛用于电力、石化、机械、冶金等行业及各种能源计量领域[详细]

  2020-10-16 09:41

  期刊论文

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