SLPe使用说明书（英文）

本文由 必能信超声（上海）有限公司 整理汇编

2012-07-09 00:00 1096阅读次数

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malachitegreen（MG）ELISA1UsepurposeThiskitforFeed,aquaticsamples,watersamplesintheMGremainingquantitativedetection.2ExperimentalprincipleThiskitadoptsmethodsincompetitionELISAInmicroplatescoatedwithantigenconjugatedMG,addMGstandardorsamples,FreeMGandpre-coatedonstripsofMGconjugatedantigencompeteagainstMGantibodyconjugates,WithTMBchromogenicsubstrate,thecolorischangedfrombluetoyellowafteraddingstopsolution,enzymestandardinstrument450nmwavelengthsintesting,absorblightvalueandinthesamplewasMGcontentisinverselyproportionaltothestandardcurve,throughcalculationsampleswasMGconcentrations.3Materialsprovidedwiththekit3.1Microelisastripplate:1block（12well×8strips）.3.2MGstandard:sixvialsof（1ml/vial）,contentisrespectively:0PPB,0.1PPB,0.3PPBand0.9PPB,2.7PPB,8.1PPB.3.3Anti-MGantibodyconjugate:1vial（6ml）.3.4ChromogenSolutionA:1vial（6ml）.3.5ChromogenSolutionB:1vial（6ml）.3.6StopSolution:1vial（6ml）,2Msulphuricacid.3.7sampledilution:1vial（10x,6ml）,usedforsampledilutedwith.3.8washsolution:1vial（20x,20ml）,usedforwashingboard.3.9Instruction.4Neednotprovidematerials4.1equipment4.4.1wavelength450nmmicroplate.4.1.2shredder.4.1.3LiangTong.4.1.4oscillators.4.1.5funnel.4.1.6WhatmanNo1orequivalentfilterpaper.4.1.7traceremoveliquiddevice.4.2reagent4.2.1thedeionizedwaterordistilledwater.4.2.2methanol.5Storage5.1kitstoredin2~8℃,Don’tfrozen5.2Don’tuseuptheMicroelisastripplate首ldbesealeddryingpreserve6.Precautions6.1pleasereadtheinstructionscarefully,beforeuseingthekit.6.2don'tuseexpiredkit.6.3beforeusingthekit,pleaseletthereagentrecovertoroomtemperature（25+2℃）,theproposalforatleast2hourstotemperature.6.4thestandardcontainMG,payspecialattentionto,theoperation,we首ldbringgloves.6.5stopsolutioniscontainingsulfate,whenusing,preventburnsskinandcorrosionclothing.6.6differentstandardandsamplesuctionheadusedcannotbemixeduse,otherwise,itwillaffecttestresults.6.7differentbatchesofreagentkitnotmix,Differentstandardandsamplesuctionheadshallnotbeusedincombination,otherwise,itwillaffecttheexperimentalresult.6.8dilutedsamplemustusethiskitofsamplediluent,otherwise,itwillaffecttheexperimentalresult6.9mixedreagents首ldavoidblistering.7Workingliquidpreparation7.1Carbendazimstandards:0ppb,0.1PPB,0.3PPBand0.9PPB,2.7PPB,8.1PPB7.2washsolution:1:20withdistilledwater（1+19）diluted.preparation7.3sampledilutions:1:10withdistilledwater（1+9）diluted.preparation7.3ChromogenSolutionreagent:alreadystandby,avoidlightstraightas7.4stopsolution:alreadyterminationaside8Sampleprocessingprogram（sampleinextractionprocess,muststrictlyaccordingtotheoperationoftheextractionprocess首ldbeaccuratedilution,canappearotherwiseresultsareinaccurate,samples首ldbekeptinacoolplacetoavoidlightandfrozenkeep）8.1Smashthesamplestaken10g,add20ml70%methanolsolution8.2powerfuloscillation3minutes8.3WhatmanNo1filterwith8.4Takethe25μltreatmentofthesamplebyadding25μlsampledilutioninthewells（sampledilutionfactorof2）9Enzyme2lindedanalysissteps9.1experimentalguidelines9.1.1experimentbeginspriortoallreagentinboxesoutsidetheroomtemperature（25fullyrecoveredto+2℃）,timeabout2hours.Returntoroomtemperature（25+2℃）againafterremoveStrips,excessporebartosealimmediatelytothe2~8℃dryingpreserveNote:besuretotemperature,otherwisefullyguaranteetheaccuracyandprecisionoftheaffectingdetection.9.1.2afterusepleaseimmediatelyreagentputback2~8℃preservation9.1.3pleasedon'tchangeanalysisprogram9.1.4pleaseuseaccuratetraceremoveliquiddevice9.1.5operationoncestarted,pleasedonotinterruptanyprogram9.1.6ELISAresultsofrepeatabilityofseveredependsonoperatingprocedures,pleasestrictlyaccordingtorequirementsoperation9.1.7toavoidcross-contamination,eachstandardandsamples首ldusedifferentsuctionheadaddsamples9.1.8plussampledomakesuckaheadcontactmicroporousthesolutionorinsidesurface9.2analysissteps9.2.1beforehandnumbered,markB0,standardandthesamplepositionrecommendeddoubleorificedetection9.2.2taketherequiredamountoftheStrips（Stripsdetachable）,willspareattribwattleandsealedimmediatelyputbackagain2~8℃preservation9.2.3sampledilutions（10）,washsolutionx（20x）dilutionintoworkingliquid（distilledwaterordeionizedwaterdilute）In9.2.4B0welljoining50μl0.0ng/mlstandard9.2.5ineachstandardwelljoining50μlstandard9.2.6ineachsamplewelljoining50μlsamplesolution9.2.7Inallwelljoining50μlAnti-MGantibodyconjugate9.2.8gentlysloshingresponseboardforafewseconds.9.337℃warmbath30min（warmbathprocesssometimespatreactionplate,canreducedoubleorificeerror）9.3.1UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat..9.4reaction9.4.1washingprocedurecompleted,immediatelywithliquidapparatusineverytracemovemicroporousfirstjoin50μlChromogenSolutionA,add50μlChromogenSolutionB,Slightsloshingresponseboardmakethoroughlyincorporated379.4.2℃warmbath10min9.4.3eachwelljoining50μlStopSolution,blending9.4.4in450nmtestingabsorbency,resultin5mininsideread.10Theresultscalculated10.1quantitativeanalysis10.1.1obtainedbyeachconcentrationstandardsolutionandtheaveragevalueofasamplespectrophotometry（B）dividedbythefirststandard（0standardabsorbencyvalue（B0）multiplyingby1**%,namelypercentageabsorbencyvalues.B-standardsolutionorsamplesolutionofaverageabsorbanceofthevaluesB0-0μg/Lstandardsolutionofaverageabsorbanceofthevalues10.1.2withMGconcentrationsofvaluesfortheXaxis,100centabsorbanceofthevalueoftheYaxis,drawstandardcurve.Accordingtothesamplepercentageabsorbencyvalues,whichgetcorrespondingpointsfromcurve,namelytheabscissadenotesthemulti-goalMGconcentrationonthenumericalcurvature.theagainstseveralnamelytodetermineMGconcentrationC（ppb）10.1.3becausethesampleafterdilutedinadvance,soaccordingtothestandardcurvegainsfromdifferentconcentrationsamplesmustagainmultiplythedilutedtimes.10.2halfquantitatively10.1.1visualhalfquantitativedetermination:first,chooseanappropriatestandardfluidandsampleswithoperation,accordingtothesamplesandstandardsubstanceabsorbencyvaluethediscretionofthejudgeiscompared,samplechromavalueislessthanorgreaterthanstandardvalues.10.1.2instrumenthalfquantitativedetermination:first,chooseanappropriatestandardfluidandsampleswithoperation,accordingtothesampleandstandardcolordepthcomparison,judgesamplechromavalueislessthanorgreaterthanstandardvalues.11SpecificityPhysicalcrossreactionMG1**%CrystalViolet:95%Recessivemalachitegreen:0.1%HiddenCrystalViolet:<0.1%12KitparametersThiskitdetectionlimitis0.05PPBB0absorbanceoftheoptimalvalue首ldbegreaterthan1.0Kitabsorbencyboardinsideerrorislessthan8%,boardbetweenerrorislessthan15%.Withthismanualprovidedatissuesampleextractionmethodrecoveryisgreaterthan80%.13AnalysisrestrictionThiskittestingpositiveforsamples首lduseanothermethodsuchasHPLCorGC/MStobeverified.上海恒远生物科技有限公司，专业销售“孔雀石绿试剂盒”品质保证，值得信赖！如果你想了解该产品的详细信息，欢迎来电来函！[详细]

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• 便携式地磅（汽车衡）英文说明书

  概述行驶道路的车辆超限超载是造成道路加速损坏以及引发交通事故的潜在重要原因，随着《道路交通安全法》的实施，我国交通和JJ等相关部门对超限超载的执法力度不断加强，同时相关执法部门更需要一种能够在其执法过程中极ng确检测车辆动态重量的高质量设备。为了给相关执法管理部门提供一个便捷、GX的执法工具，提供有力的执法依据，根据交通部及有关部委对车辆轴类型和轴载质量的相关规定标准，我公司于1999年初自主研发生产出符合我国车辆行驶公路管理规范要求的便携式称重仪（轴重仪），为交通管理部门实施有效的超限超载治理提供了强有力的执法工具。您所使用的便携式称重仪是我公司基于自身多年的实际动态称重经验以及客户的实际需要，而Zxin研发生产的JJ系列新一代全数字便携式称重仪（轴重仪），旨在改善提高目前市场上同类产品不能满足用户的实际使用需求而研发生产，具有多项创新功能，主要体现在：1、采用7寸全彩触摸式大屏显示，完全满足用户在夜间操作的方便性2、国内SJ将电脑技术应用于便携式动态称重仪领域，即采用用户熟悉的鼠标操作方式，使得产品实用性更强，操作更简单明了，大大提高操作人员的工作效率。3、国内SJ推出JJ和路政联合执法模式功能4、国内SJ推出功能性更强的后台软件管理功能，只要把我们的仪表与安装有后台管理软件的计算机连接，就可以实时的控制仪表操作，为更高功能的扩展打下基础产品设计定位高、精度高、性能稳定、使用可靠、环保节能、故障率低、维修简捷等特点。可方便的对机动车进行轴载质量、车货总质量的动、静态测量。适用于公路执法部门超限检查、JJ进行车载质量检查、高速公路收费站进行车载质量计量等，也适用于低值车载货物计量，如交通运输车载质量计量、建筑施工工地配料计量等场合。一、主要功能特点:（1）、传感器采用一体化整体式结构整体式传感器（2）、数据传输方式：有线式、无线式、有线无线两用式（具体传输方式根据用户的实际需要在采购是选择而定）（3）、采用7寸彩色触摸式屏幕显示，实用（4）、可以使用触摸方式输入操作和无线鼠标方式操作，简单快捷（5）、多种工作（JJ、路政、综合）模式可供选择（6）、动、静两用、性能稳定、具有高性能防水、防振、防腐等特点（7）、双通道式设计、使用高精度整体式传感器，检测精度高，故障率低（8）、统计分析软件，便于记录、统计、查询；数据库提供车型资料、政策法规、技术支持（9）、即时电脑连接操作，可以通过电脑和仪表连接直接操作，并可实现视频和车牌自动识别功能扩展功能强大（此功能为选配）二、主要技术参数:1、名称型号：便携式称重仪（JJB30BW型）2、静态精度：静态称重（III）；±0.1～0.3%FS蠕变：0.3%.F.S，迟滞：0.5%.F.S3、动态精度：OIML国际建议级1%-3%F.s非线性：0.5%.F.S，重复性：0.3%.F.S4、分度值：5、10、20kg等可调节5、Zda称重量：40000kg（单轴）6、Z小秤量：20e（e为检定分度值）7、Zda安全载荷：安全超载能力：120%F.SZda超载能力：150%F.s8、使用环境：-20~80℃9、电源：DC12V、AC220V、车载供电三种供电方式10、引板：橡胶引板11、限制车速：≤5km/h匀速12、打印内容：牌照、车货总质量、轴胎形式、限载值、超限值、时间等（具体打印内容依据客户的要求而定）13、软件：具有3种称重软件（路政、JJ、综合模式）且可以自由切换14、数据输出：RS232、USB两种数据接口15、传感器结构：采用整体数字传感器，温度补偿，可以在秤台温度变化较大时对温度产生的误差进行补偿，同时传感器采用双信号电路设计，在一个电路出现故障的同时，系统会自动切换到另外一个电路，提高现场设备的实用性，减少设备的故障率16、轴型表模式：轴型表采用内部电子显示方式设定，彻底解决长期野外使用贴表式轴型表模糊不清以及操作不便的问题17、视频：支持即时视频联接、支持车牌自动识别系统、支持后台软件扩展（此功能为选配）18、打印机:采用针式节能高速打印机，可保存打印内容，彻底解决热敏打印机高耗电、而且打印内容容易自然消退的问题电子秤（上海电子秤）地磅秤（小地磅秤）大台面地磅秤（电子磅秤）吊秤（上海吊秤）[详细]

  2024-09-30 17:41

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• NDJ-8S数显旋转粘度计英文说明书

  NDJ-8S数显旋转粘度计英文说明书[详细]

  2009-10-13 00:00

  报价单

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• 人L-精氨酸（L-ARG）英文说明书

  HumanL-Arginine（L-ARG）FORRESEARCHUSEONLYAssayrange：2nmol/L-48nmol/L96determinationsPurposeThiskitallowsforthedeterminationofL-ARGconcentrationsinHumanserum,cellculturesupernatesandotherbiologicalfluidsPrincipleoftheassayThekitassayHumanL-ARGlevelinthesample,usePurifiedHumanL-ARGantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddL-ARGtowells,CombinedL-ARGantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofHumanL-ARGinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.Materialsprovidedwiththekit1washsolution20ml×1bottle7StopSolution6ml×1bottle2HRP-Conjugatereagent6ml×1bottle8Standard（96nmol/L）0.5ml×1bottle3Microelisastripplate12well×8strips9Standarddiluent1.5ml×1bottle4Samplediluent6ml×1bottle10Instruction15ChromogenSolutionA6ml×1bottle11Closureplatemembrane26ChromogenSolutionB6ml×1bottle12Sealedbags1Specimenrequirements1.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.2.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.Assayprocedure1.Diluteandaddsample:DiluteOriginaldensityStandardasfollowtable:48nmol/L5Standard150μlOriginaldensityStandard+150μlStandarddiluent24nmol/L4Standard150μl5Standard+150μlStandarddiluent12nmol/L3Standard150μl4Standard+150μlStandarddiluent6nmol/L2Standard150μl3Standard+150μlStandarddiluent3nmol/L1Standard150μl2Standard+150μlStandarddiluent2.Addsample:Setblankwellsseparately(blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame).testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl(samplefinaldilutionis5-fold),addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-fold(or20-fold)washsolutiondiluted30-fold(or20-fold)withdistilledwaterandreserve.5.Washing:UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.Addenzyme:AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.Incubate:Operationwith3.8.Washing:Operationwith5.9.Color:AddChromogenSolutionA50ulandChromogenSolutionBtoeachwell,evadethelightpreservationfor15minat37℃10.Stopthereaction:AddStopSolution50μltoeachwell,Stopthereaction(thebluecolorchangetoyellowcolor).11.Assay:takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.StepsdescriptionStandard,SamplediluentAddStandard,Samplediluent,incubatefor30minat37℃.Wash5time,AddHRP-Conjugatereagent,incubatefor30minat37℃.Wash5times,AddChromogenSolutionAandB,incubatefor30minat37℃.AddStoppSolutionReadabsorbanceat450nmwithin15mincalculateCalculateTakethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.Importantnotes1.Thekittakesoutfromtherefrigerationenvironment首ldbebalanced15-30minutesintheroomtemperature,ELISAplatescoatedifhasnotuseupafteropened,theplate首ldbestoredinSealedbag.2.washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.3.addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.4.ifthetestingmaterialcontentisexcessivelyhigher(ThesampleODisbiggerthanthefirststandardwell),pleasediluteSample(n-fold),Pleasediluenteandmultipliedbythedilutionfactor.（×n×5）.5.Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.6.Thesubstrateevadethelightpreservation.7.Pleaseaccordingtouseinstructionstrictly,Thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.8.Allsamples,washingbufferandeachkindofreject首ldaccordingtoinfectivematerialprocess.9.Donotmixreagentswiththosefromotherlots.Storageandvalidity1．Storage：2-8℃.2．validity：sixmonths[详细]

  2018-09-19 10:00

  产品样册

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• 猪DC细胞（DC）英文说明书

  PorcineDCFORRESEARCHUSEONLYAssayrange：1.0ng/L-60ng/L96determinationsPurposeThiskitallowsforthedeterminationofDCconcentrationsinPorcineserum,cellculturesupernatesandotherbiologicalfluidsPrincipleoftheassayThekitassayPorcineDClevelinthesample，usePurifiedPorcineDCantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddDCtowells,CombinedDCantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofPorcineDCinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.Materialsprovidedwiththekit1washsolution20ml×1bottle7StopSolution6ml×1bottle2HRP-Conjugatereagent6ml×1bottle8Standard（120ng/L）0.5ml×1bottle3Microelisastripplate12well×8strips9Standarddiluent1.5ml×1bottle4Samplediluent6ml×1bottle10Instruction15ChromogenSolutionA6ml×1bottle11Closureplatemembrane26ChromogenSolutionB6ml×1bottle12Sealedbags1Specimenrequirements1.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.2.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.Assayprocedure1.Diluteandaddsample:DiluteOriginaldensityStandardasfollowtable:60ng/L5Standard150μlOriginaldensityStandard+150μlStandarddiluent30ng/L4Standard150μl5Standard+150μlStandarddiluent15ng/L3Standard150μl4Standard+150μlStandarddiluent7.5ng/L2Standard150μl3Standard+150μlStandarddiluent3.75ng/L1Standard150μl2Standard+150μlStandarddiluent2.addsample：Setblankwellsseparately(blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame).testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl(samplefinaldilutionis5-fold),addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-fold(or20-fold)washsolutiondiluted30-fold(or20-fold)withdistilledwaterandreserve.5.washing：UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.addenzyme：AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.incubate：Operationwith3.8.washing：Operationwith5.9.color：AddChromogenSolutionA50ulandChromogenSolutionB50ultoeachwell,evadethelightpreservationfor10minat37℃10.Stopthereaction：AddStopSolution50μltoeachwell,Stopthereaction(thebluecolorchangetoyellowcolor).11.assay：takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.StepsdescriptionStandard,SamplediluentAddStandard,Samplediluent,incubatefor30minat37℃.Wash5time,AddHRP-Conjugatereagent,incubatefor30minat37℃.Wash5times,AddChromogenSolutionAandB,incubatefor10minat37℃.AddStopSolutionReadabsorbanceat450nmwithin15mincalculateCalculateTakethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.Importantnotes1.Thekittakesoutfromtherefrigerationenvironment首ldbebalanced15-30minutesintheroomtemperature,ELISAplatescoatedifhasnotuseupafteropened,theplate首ldbestoredinSealedbag.2.washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.3.addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.4.ifthetestingmaterialcontentisexcessivelyhigher(ThesampleODisbiggerthanthefirststandardwell),pleasediluteSample(n-fold),Pleasediluenteandmultipliedbythedilutionfactor.（×n×5）.5.Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.6.Thesubstrateevadethelightpreservation.7.Pleaseaccordingtouseinstructionstrictly,Thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.8.Allsamples,washingbufferandeachkindofreject首ldaccordingtoinfectivematerialprocess.9.Donotmixreagentswiththosefromotherlots.Storageandvalidity1．Storage：2-8℃.2．validity：sixmonths[详细]

  2018-09-19 10:00

  产品样册

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• 人活化凝血因子Ⅻ（FⅫa）英文说明书

  HumanActivatedcoagulationfactorⅫ(FⅫa)FORRESEARCHUSEONLYAssayrange：2.2U/L-80U/L96determinationsPurposeThiskitallowsforthedeterminationofFⅫaconcentrationsinHumanserum,cellculturesupernatesandotherbiologicalfluidsPrincipleoftheassayThekitassayHumanFⅫalevelinthesample,usePurifiedHumanFⅫaantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddFⅫatowells,CombinedFⅫaantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofHumanFⅫainthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.Materialsprovidedwiththekit1washsolution20ml×1bottle7StopSolution6ml×1bottle2HRP-Conjugatereagent6ml×1bottle8Standard（160U/L）0.5ml×1bottle3Microelisastripplate12well×8strips9Standarddiluent1.5ml×1bottle4Samplediluent6ml×1bottle10Instruction15ChromogenSolutionA6ml×1bottle11Closureplatemembrane26ChromogenSolutionB6ml×1bottle12Sealedbags1Specimenrequirements1.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.2.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.Assayprocedure1.Diluteandaddsample:DiluteOriginaldensityStandardasfollowtable:80U/L5Standard150μlOriginaldensityStandard+150μlStandarddiluent40U/L4Standard150μl5Standard+150μlStandarddiluent20U/L3Standard150μl4Standard+150μlStandarddiluent10U/L2Standard150μl3Standard+150μlStandarddiluent5U/L1Standard150μl2Standard+150μlStandarddiluent2.Addsample:Setblankwellsseparately(blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame).testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl(samplefinaldilutionis5-fold),addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-fold(or20-fold)washsolutiondiluted30-fold(or20-fold)withdistilledwaterandreserve.5.Washing:UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.Addenzyme:AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.Incubate:Operationwith3.8.Washing:Operationwith5.9.Color:AddChromogenSolutionA50ulandChromogenSolutionB50ultoeachwell,evadethelightpreservationfor10minat37℃10.Stopthereaction:AddStopSolution50μltoeachwell,Stopthereaction(thebluecolorchangetoyellowcolor).11.Assay:takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.StepsdescriptionStandard,SamplediluentAddStandard,Samplediluent,incubatefor30minat37℃.Wash5time,AddHRP-Conjugatereagent,incubatefor30minat37℃.Wash5times,AddChromogenSolutionAandB,incubatefor10minat37℃.AddStopSolutionReadabsorbanceat450nmwithin15mincalculateCalculateTakethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.Importantnotes1.Thekittakesoutfromtherefrigerationenvironment首ldbebalanced15-30minutesintheroomtemperature,ELISAplatescoatedifhasnotuseupafteropened,theplate首ldbestoredinSealedbag.2.washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.3.addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.4.ifthetestingmaterialcontentisexcessivelyhigher(ThesampleODisbiggerthanthefirststandardwell),pleasediluteSample(n-fold),Pleasediluenteandmultipliedbythedilutionfactor.（×n×5）.5.Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.6.Thesubstrateevadethelightpreservation.7.Pleaseaccordingtouseinstructionstrictly,Thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.8.Allsamples,washingbufferandeachkindofreject首ldaccordingtoinfectivematerialprocess.9.Donotmixreagentswiththosefromotherlots.Storageandvalidity1．Storage：2-8℃.2．validity：sixmonths[详细]

  2018-09-19 10:00

  产品样册

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