Rat Anti- Goat Brucella Antibody

Rat Anti- Goat Brucella Antibody

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2024-09-20 03:03 359阅读次数

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Rat Anti- Goat Brucella Antibody

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Rat Anti- Goat Brucella Antibody

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Anti-β-Actin

Anti-β-Actinβ-肌动蛋白抗体（内参抗体）规格：0.1ml/0.2mlAnti-β-ActinCAS号:932-66-1,1-乙酰环己烯?CAS号:126-81-8,双甲酮,CP,96%MouseSolubleEndoglin,ENG/sCD105ELISAKit人发动蛋白2厂家电话(DNM2)Elisa试剂盒哪里**人细胞色素P450c21B/21-羟化酶Elisa试剂盒Elisa试剂盒供货商,(CYP21B)ELISA试剂盒,96孔|48孔小鼠S100蛋白实验(S-100)Elisa试剂盒*** 大鼠前列腺酸性磷酸酶Elisa试剂盒进口Elisa和国产试剂盒的区别,(PAP)ELISA试剂盒检测范围是多少,96孔|48孔大鼠血小板衍生生长因子AB实验(PDGF-AB)Elisa试剂盒*** CAS号:15510-55-1,十二烷基三苯基溴化膦,98%人细胞绒毛蛋白/埃兹蛋白厂家电话(cytovillin/ezrin)Elisa试剂盒哪里**人成纤维细胞生长因子23Elisa试剂盒进口Elisa和国产试剂盒的区别,(FGF-23)ELISA试剂盒检测范围是多少,96孔|48孔CAS号:95-01-2,2,4-二羟基苯甲醛,98%Mousemyelinbasicprotein,MBPELISAKit绵羊主要组织相容性复合体实验(MHC)Elisa试剂盒*** 小鼠黑色素细胞抗体厂家电话(MCAb)Elisa试剂盒哪里**小鼠神经生长导向因子Slit2Elisa试剂盒*** 人巨噬细胞移动YZ因子Elisa试剂盒Elisa试剂盒供货商,(MIF)ELISA试剂盒检测范围是多少,96孔|48孔CAS号:52417-22-8,9-氨基吖啶盐,99%Anti-β-Actin[详细]
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Anti-Streptavidin antibody （FITC）

ProductoverviewDescriptionRabbitpolyclonaltoStreptavidin（FITC）ConjugationFITCConjugationnotesFluoresceinisothiocyanate（FITC）（MolecularWeight390daltons）AbsorptionWavelength:495nmEmissionWavelength:528nmFluorochrome/ProteinRatio:4.0molesFITCpermoleofRabbitIgGHostspeciesRabbitSpecificityNocrossreactivityoccursagainstAvidin.TestedapplicationsImmunomicroscopy,FlowCytImmunogenStreptavidin[Streptomycesavidinii]TargetRelevanceStreptavidinisatetramericproteinpurifiedfromStreptomycessp.thatbindsverytightlytothevitaminbiotinwithaKdof~10-14mol/l.Thehighaffinityrecognitionofbiotinandbiotinylatedmoleculeshasmadestreptavidinoneofthemostimportantcomponentsindiagnosticsandlaboratorykits.CellularlocalizationCytoplasmicAlternativenamesSAV1antibodySAV2antibodyStreptavidinV1antibodyStreptavidinV2antibodyPropertiesFormLiquidStorageinstructionsStoreat+4°Cshortterm（1-2weeks）.Aliquotandstoreat-20°Cor-80°C.Avoidrepeatedfreeze/thawcycles.Storagebuffer0.02MKPhosphate,0.15MNaCl,0.01%SodiumAzide,pH7.2SeethewebsiteformoreSDSinformationforthisproduct.PurityIgGfractionPurificationnotesThisproductisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysis.Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-rabbitserumandstreptavidin.ClonalityPolyclonalIsotypeIgGApplicationsImmunomicroscopy,FlowCytTherearenonoteslistedasspecifictotheseapplications.Formoreinformationpleaseseethe'generalapplicationnotes'sectionbeloworcheckthewebsite.GeneralapplicationnotesSuitableforimmunomicroscopyandflowcytometryorFACSanalysisaswellasotherantibodybasedfluorescentassaysOurAbpromisetoyou:QualityguaranteedandexperttechnicalsupportIftheproductdoesnotperformasdescribedonthisdatasheet,wewillofferarefundorreplacement.ForfulldetailsoftheAbpromise,pleasevisit[详细]
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Rabbit Anti-PTEN antibody

RabbitAnti-PTENantibodyCatalogNumber:BYK-0748RQuantitysize:100ug(0.01MPBS,pH7.4with10mg/mlBSAand0.1%Sodiumazide)Background:Potentialtumorsuppressor.Actsasaphosphoinositide3-phosphatasebyregulatingPtdIns(3,4,5)P3levels.InvolvedinregulationoftheAKT1signa领pathway.TheunphosphorylatedformcooperateswithAIP1tosuppressAKT1activation.ThePTENdiscoversthefirsttohavethesuppressofthephosphoricacidenzymeactivitycancergenecurrently.ThegeneofPTENlocatesthechromosome10q23area,sendingforthsextumorandafewhouseholdscancerswiththevarietytosufferfromthecomprehensivediseaseeasilyrelevant.TheactivitythatpassestorepresstheAktregulatesthecellperiod,thecellgroundruledeceaseandgluestoconnect.ThistextdiscussedPTENstructure,functionanditscorrelationses,thePTENisintumorrepressfunctionmechanism.RabbitAnti-PTENantibodySpecificity:Anti-PTENisarabbitpolyclonalantibodyunconjugatedspecificforPTENofHuman,Mouse,Ratuseforwesternblotting,elisa,immunoprecipitationandimmunohistochemistryProteinGaffinitychromatographypurification,purity:>95%Isotype:IgGmolwt:44kDaApplication:Westernblotting1:100-500Immunohistochemistry1:100-500ELISA1:500-1000IP=1:20-100IF=1:100-500Optimalworkingdilutionsmustbedeterminedbytheenduser.RabbitAnti-PTENantibodyStorage:Storeat-20℃foroneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20℃.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibody,theantibodyisstableforatleastsixweeksat2-4℃ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.RabbitAnti-PTENantibody更多相关抗体：Camk1g(Calcium/calmodulindependentproteinkinaseIG)钙/钙调蛋白依赖蛋白激酶IG抗体CaMK2b（calcium/calmodulin-dependentproteinkinaseIIbeita）钙/钙调素依赖蛋白激酶2b抗体CAP1/Park7/DJ-1Park7/DJ-1/CAP1抗体CAP2（Adenylylcyclase-associatedprotein2）环化酶相关蛋白CAP-2抗体CTn1(Cardiactroponin1)心肌肌钙蛋白抗体CT-1/CTF1(Cardiotrophln1)心肌营养素1抗体CAI(CarbonicanYMdraseI)碳酸酐酶1抗体CTNNAL1(catenin（cadherin-associatedprotein）alpha-like1)粘附分子相关蛋白a1抗体CAⅡ(CarbonicanYMdraseⅡ)碳酸酐酶2抗体CAS（CellularApoptosisSusceptibility）细胞凋亡敏感性基因Caspase-1(ICE/CASP-1/P45)天冬氨酸-胱氨酸特异性蛋白酶家族抗体Caspase-10半胱胺酸蛋白酶-10抗体Caspase-12（ratmouse）半胱胺酸蛋白酶蛋白-12抗体（大、小鼠）Caspase-12（hunman）半胱胺酸蛋白酶蛋白-12抗体()Caspase-13半胱胺酸蛋白酶蛋白-13抗体Caspase-3(Active)/caspase-3p17subunit活化半胱胺酸蛋白酶蛋白-3抗体caspase-3p12subunit活化半胱胺酸蛋白酶蛋白-3抗体procaspase3半胱天冬酶-3酶原抗体CASP4(Caspase-4)半胱胺酸蛋白酶蛋白-4抗体Caspase-6（CT）半胱胺酸蛋白酶蛋白-6抗体（C端）Caspase-6（NT）半胱胺酸蛋白酶蛋白-6抗体(N端)Caspase-6（NT）mouse半胱胺酸蛋白酶蛋白-6抗体(N端)Caspase-8(proMch5)半胱氨酸蛋白酶8抗体Caspase-9白介素1-β转化酶样凋亡蛋白酶6抗体Caspase-9白介素1-β转化酶样凋亡蛋白酶6抗体[详细]
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Goat Anti-Mouse α-glucosidase

GoatAnti-Mouseα-glucosidaseStorage:2-8°CPackagesize:96determinationsPRINCIPLEOFTHEMETHODTheα-glucosidasekitisasolidphasephasesandwichenzymelinkedimmunosorbentassay(ELISA).Samples,includingstandardsofknownα-glucosidaseconcentrationsandunknownsarepipettedintothesewells.Duringthefirstincubation,theα-glucosidaseantigenandabiotinylatedmonoclonalantibodyspecificforα-glucosidasearesimultaneouslyincubated.Afterwashing,theenzyme(streptavidin-peroxydase)isadded.Afterincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionwhichisactingontheboundenzymeisaddedtoinduceacolouredreactionproduct.Theintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofα-glucosidasepresentinthesamples.REAGENTSPROVIDEDANDRECONSTTTUTIONREAGENTS（Storeat2-8℃）1×96WELLS0.5×96WELLSRECONSTTTUTION96/48-wellsmicrotiterplates10.5Ready-to-usePlastivcover21Ready-to-useStandard:400nmol/L1Vials(0.6ml)0.5Vials(0.3ml)Seereagentspreparationonpage3Blankcontrol1Vials(1.0ml)1Vials(0.5ml)Ready-to-useStandardDiluent1Vials(4.0ml)1Vials(2.0ml)Ready-to-useBiotinylatedanti-α-glucosidase1Vials(6.0ml)1Vials(3.0ml)Ready-to-useStreptavidin-HRP1Vials(8.0ml)1Vials(4.0ml)Ready-to-useWashingBuffer1Vials(20ml)1Vials(10ml)50×concentrateSubstrateA1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSubstrateB1Vials（6.0ml)1Vials(3.0ml)Ready-to-useStoppingSolution1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSampleDiluent1Vials(12ml)1Vials(6.0ml)Ready-to-useGoatAnti-Mouseα-glucosidaseMATERIALREQUIREDBUTNOTPROVIDED?Distilledwater?Pipettes:10ul、50ul、100ul、200ul、1000ul。?Vortexmixerandmagneticstirrer.SAFETY?Forresearchuseonly?AvoidanyskincontactwithH2SO4andTMB.Incaseofcontact,washthoroughlywater.?Donoteat,drink,smokeorapplycosmeticswherekitreagentsareused.?Donotpipettebymouth.PROCEDURALNOTES/LAB.QUALITYCONTROL?Whennotinuse,kitcomponents首ldbestoredrefrigeratedorfrozenasindicatedonvialsorbottles.Allreagents首ldbewarmedtoroomtemperaturebeforeuse.Lyophilizedstandards首ldbediscardedafteruse.?Oncethedesirednumberofstripshasbeenremoved,immediatelyresealthebagtoprotecttheremainingstripsfromedterioration.?Coverorcapallreagentswhennotinuse.?Donotmisorinterchangereagentsbetweendifferentlots.?Donotusereagentsbeyondtheexpirationdateofthekit.?Useacleandisposableplasticpipettetipforeachreagent,standard,orspecimenadditioninordertoavoidcross-contamination,forthedispensingofH2SO4andsubstratesolution,avoidpipetteswithmetalparts.?Useacleanplasticcontainertopreparethewashingsolution.?Thoroughlymixthereagentsandsamplesbeforeusebyagitationorswir领.?Allresidualwashingliquidmustbedrainedfromthewellsbyefficientaspirationorbydecantationfollowedbytappingtheplateforcefullyonabsorbentpaper.Neverinsertabsorbentpaperdirectlyintothewells.?TheTMBsolutionislightsensitive.Avoidprolongedexposuretolight,also,avoidcontactoftheTMBsolutionwithmetaltopreventcolourdevelopment.WarningTMBistoxicavoiddirectcontactwithhands.Disposeoffproperly.Ifadarkbluecolourdevelopswithinafewminutesafterpreparation,thisindicatesthattheTMBsolutionhasbeencontaminatedandmustbediscarede.Readabsorbanceswithin1houraftercompletionoftheassay.?Whenpipettingreagents,maintainaconsistentorderofadditionfromwell-to-well.Thiswillensureequalincubationtimesforallwells.?Respectincubationtimesdescribedintheassayprocedure.SPECIMENCOLLECTION\PROCESSINGANDSTORAGE?Serum---Avoidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serum首ldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.?Plasma---EDTA\citrateandheparinplasmacanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestplasma.?Cellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.?Storage---Ifnotanalyzedshortlyaftercollection,samples首ldbealiquoted(250-500ul)toavoidfreeze-thawcyclesandstoredfrozenat-70℃.Avoidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,avoiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,this首ldberemovedpriortoassaybycentrifugationorfiltration.?Recommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbeforeassaying.PREPARATIONOFREAGENTS?Standards:Standardhavetobereconstituledwiththevolumeofstandardbufferdiluentindicatedonthevial.Thisreconstitutionproducesastocksolutionof400nmol/Lα-glucosidase.Allowstandardtostandfor5?minuteswithgentleswir领priortomakingdilutions.Serialdilutionsofstandardmustbemadebeforeeachassysandcannotbestored.400nmol/L（6Standard）Originaldensity50ul。200nmol/L（5Standard）100ul6Standard+100uldiludent100nmol/L（4Standard）100ul5Standard+100uldiludent50nmol/L（3Standard）100ul4Standard+100uldiludent25nmol/L（2Standard）100ul3Standard+100uldiludent12.5nmol/L（1Standard）100ul2Standard+100uldiludent0nmol/LBlankControl50ul。?Washingbuffer50×concentrate:Dilute50timesindistilledwater.ASSAYMETHOD?Beforeuse,mixallreagentsthoroughlywithoutmakingfoam.?Determinethenumberofmicrowellstripsrequiredtotestthedesirednumberofsamples,plusappropriatenumberofwellsneededforrunningblanksstandards.Eachsample,standardandblank首ldbeassayedinduplicate.Removesufficientmicrowellstripsfromthepouch.?Add50ulofstandarddiluenttostandardwellsB1,B2,C1,C2,D1,D2,E1,E2,F1,F2.Reconstitutestandardvialwiththeappropriatevolumeasdescribedinthechapterreagentspreparation.Preparation.Pipet100ulofstandardintowellsA1andA2(seeplateschemebelow).Transfer50ulfromA1andA2toB1andB2wells.Mixthecontentsbyrepeatedaspirationsandejections.Takecarenottoscratchtheinnersurfaceofmicrowells.RepatthisprocedurefromthewellsB1,B2towellsC1,C2andfromwellsC1,C2toD1,D2andsooncreatingtwoparallelrowsofα-glucosidasestandarddilutionsranging，Add50ulofstandarddiluenttotheblandwells.?Dilutesamples1:1distribing50ulofsampleinto50ulofdilluent,Add50ulofdilutedsampletowells..?Add50ulofdilutedbiotinylatedanti-α-glucosidasetoallwells.?Coverwithaplatevoverandincubatefor1hourat37℃.?Removethecoverandwashtheplateasfollows:⑴aspiratetheliquidfromeachwell,⑵dispensse0.3mlofwashingsolutionintoeachwell.⑶Aspirateagainthecontetofeachwellafter0.5minute.⑷Repeatsteps⑵and⑶threetimes.?Distribute60ulofstreptavidin-HRPsolutiontoallwells,includingblankwells.?Coverandincubate30minat37℃.?Removethecoverandemptywells,Washmicrowellstripsaccordingtostep,Proceedimmediatelytothenextstep.?Add50ulSubstrateAandSubstrateBtoeachwell。Incubatefor10minat37℃。?Theenzyme-substratereactionisstoppedbyquicklypipetting50ulofH2SO4.stopreagentintoeachwell,includingtheblankwells,tocompletelyanduniformlyinactivatetheenzyme.ResultsmustberedimmediatelyaftertheadditionofH2SO4.?Readabsorbanceofeachwellonaspectrophotometerusing450nmastheprimarywavelengthandoptionally620nm(610nmto650nmisacceptable)asthereferencewavelength.GoatAnti-Mouseα-glucosidaseSUGGESTEDPLATESCHEMEStandardconcentrations（nmol/L）A400400samplesamplesamplesamplesamplesamplesamplesamplesamplesampleB200200samplesamplesamplesamplesamplesamplesamplesamplesamplesampleC100100samplesamplesamplesamplesamplesamplesamplesamplesamplesampleD5050samplesamplesamplesamplesamplesamplesamplesamplesamplesampleE2525samplesamplesamplesamplesamplesamplesamplesamplesamplesampleF12.512.5samplesamplesamplesamplesamplesamplesamplesamplesamplesampleG00samplesamplesamplesamplesamplesamplesamplesamplesamplesampleHsamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesampleLIMITATIONSOFTHEPROCEDUREDonotextrapolatethestandardcurvebeyondthemaxstandardcurvepoint.Thedose-responseisnon-linearinthisregionandgoodaccruacyisdifficulttoobtain.CALCULATIONOFRESULTSTheminimumdetectableconcentrationinthisassayisestimatedtobe1.0nmol/L[详细]
2018-09-27 10:00
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Antibody Research 113004 说明书

HyMAXHybridomaFusion&CloningSupplement20x货号:113004规格:100mL产品描述：HyMAX杂交瘤融合与克隆补充剂20x货号:113004替代名称：杂交瘤补充剂，杂交瘤克隆因子，HCF，杂交瘤克隆补充物，HCS，杂交瘤克隆融合补体，HCFS，杂交瘤生长补充剂，HGS，杂交瘤融合和克隆加强，杂交瘤融合和克隆补充，HFCS，细胞培养杂交瘤媒介补充说明：HyMax杂交瘤融合和生长补充剂是含有各种细胞因子，生长因子和激素的条件培养基的专有混合物。在新的杂交瘤发育过程中配制成替代饲养细胞。它也可用于从液氮快速回收冷冻细胞。融合后细胞电镀期间常规使用饲养细胞。通常，饲养细胞为新开发的克隆提供许多未知的生长因子。这些包括许多涉及同种型转换和B细胞IgG分泌维持的细胞因子。从我们多年的杂交瘤发展经验，我们已经配制了这种有条件的媒体，支持更好的生长和更高频率的IgG生产克隆开发。测试杂交瘤集落形成效率和IgG分泌的维持。用法：加入5mLHyMax20x至95mL您目前用于培养细胞的工作介质，使其占5％。不要使用超过10％的HyMax。继续用这种培养基培养细胞，直到细胞生长好几代。一旦细胞生长良好，通过逐步将百分比逐步降低到1％，0.5％和0％，可以将培养物从HyMax隔离。格式：作为无菌过滤的组织培养物测试，20x浓缩液准备使用溶液。运送到客户之前切勿冻结。每个100mL瓶子足以制作2L工作介质，足以完成一个完整的杂交瘤开发项目。储存：建议在4°C至8°C的冰箱中储存，直至6个月内使用。它可以制成等分试样，并在-20°C-80°C下冷冻保存，长期在12个月内使用。避免反复冻融，这可能会导致活动减少或降水。稳定性：储存于冰箱中的4°C至8°C或冷冻储存12个月时6个月。一旦在30天内解冻使用，或根据需要进行等分和储存。不要重复冻结和解冻。运送：准备新鲜（30天内），并以蓝色冰袋液体形式运送。HyMax与其他产品的第三方独立比较：图-1用于产生IgG产生B细胞杂交瘤的克隆因子补充物的分析。脾细胞与SP2/0细胞融合，培养14天，然后分析来自培养物的上清液。通过抗Fc（γ）ELISA进行分析。IgG阳性克隆被鉴定为产生超过25ng/mLIgG的那些。特点无血清生长添加剂低蛋白含量成分完全已知Z高克隆效率优良的单克隆抗体生产无需喂养细胞品名货号规格品牌HybridomaFusionandCloningSupplement;(HFCS)1136373500110ml(50x)RocheAppliedScienceRocheAppliedScience品牌的11363735001已停产品名货号规格品牌HyMAXHybridomaFusion&CloningSupplement20x113004100mLantibodyresearchHyMAXHybridomaFusionandCloningSupplement,20XCatalogNo.:113004AlternateNames:Hybridomasupplement,Hybridomacloningfactor,HCF,hybridomacloningsupplement,HCS,hybridomacloningfusionsupplement,HCFS,hybridomagrowthsupplement,HGS,hybridomafusionandcloningbooster,hybridomafusionandcloningsupplement,HFCS,CellCultureHybridomaMediaSupplementsDescription:HyMaxHybridomaFusionandGrowthSupplementisaproprietaryblendofconditionedmediacontainingvariouscytokines,growthfactors&hormones.Itisformulatedtoreplacefeedercellsduringnewhybridomadevelopment.Itcanalsobeusedforfastrecoveryoffrozencellsfromliquidnitrogen.Feedercellsareroutinelyusedduringplatingofcellsafterfusion.Typicallyfeedercellsprovidemanyunknowngrowthfactorstothenewlydevelopingclones.TheseincludesmanycytokineswhichareinvolvedinisotypeswitchingandmaintenanceofIgGsecretionbyB-cells.Fromourmanyyearsofexperiencewithhybridomadevelopmentwehaveformulatedthisconditionedmediathatsupportbettergrowth&higherfrequencyofIgGproducingclonedevelopment.ItistestedforhybridomacolonyformingefficiencyandmaintenanceofIgGsecretion.Usage:Add5mLofHyMax20xto95mLofyourworkingmediacurrentlyusedforculturingcellstomake5%.DonotuseHyMaxatmorethan10%.Continuewithculturingcellswiththismediauntilcellshavegrownwellforfewgenerations.Oncecellshavegrownwell,culturemaybeweanedoffofHyMaxbygraduallyreducingthepercentageinstepsto1%,0.5%and0%.Format:Providedasasterilefiltered,tissueculturetested,20xconcentratereadytousesolution.Neverfrozenbeforeshippingtocustomer.Each100mLbottleisenoughtomake2Lofworkingmedia,enoughfordoingacompletehybridomadevelopmentproject.Storage:Itisrecommendedtostoreat4°C-8°Cinrefrigeratoruntilusewithin6months.Itcanbemadeintoaliquotsandfrozenstoredat-20°C-80°Cforlongtermtousewithina12monthperiod.Avoidrepeatedfreezingandthawing,whichmaycauseactivitylossandorshowprecipitation.Stability:6monthswhenstoredat4°C-8°Cinrefrigeratoror12monthswhenstoredfrozen.Oncethawedusewithin30daysoraliquotandstoreasneeded.Donotfreeze&thawrepeatedly.Shipping:Itispreparedfresh(within30days)andshippedinliquidformonblueicepacks.ThirdpartyindependentcomparisonsofHyMaxtootherproducts:Figure-1AnalysisofcloningfactorsupplementsforproductionofIgG-producingBcellhybridomas.SplenocyteswerefusedwithSP2/0cells,culturedfor14daysbeforeanalysisofsupernatantfromthecultures.Analysiswasperformedviaanti-Fc(gamma)ELISA.IgGpositivecloneswereidentifiedasthoseproducingmorethan25ng/mLIgG.我们公司Zda优势是强大的采购，1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，3：提供加急服务，一般1-2周到货，超过时限加急费全免4：价格公道，绝大部分价格有优势，当然不能保证1**%产品都是价格Zdi,因为价格Zdi意味着没有服务.5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大交大复旦，中山等100多所大学，ROCHE，阿斯利康，国药，fisher等500多家公司6：我们du家代理的品牌有：AntibodyResearchCorporation，arcticzymes，Biorelevant，AmberGen,Inc.，clemente-associates，clodronateliposomes，ColumbiaBiosciences，enzymeresearch，GeneBridgesGmbH，Genovis，AmberGen,Inc.BiotechnologyGmbH，HaematologicTechnologiesHTIHaemtech，hookelabs，Immudex，InnovativeResearchofAmerica，inspiralis，ListBiologicalLaboratories,Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-FreezBiologicals，pentapharm，progen，ProteinArk，QA-Bio,Inc，QA-Bio,IncQuickZymeBiosciences，Teknova，TriLinkBioTechnologies,Inc.，ZyagenLaboratories等7：我们还是invitrogen,qiagen,MidlandBioProductsCorporationam,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批发，欢迎合作。[详细]
2018-09-29 10:02
产品样册
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Rat myeloperoxidase

RatmyeloperoxidaseFORRESEARCHUSEONLYDrugNamesGenericName：Ratmyeloperoxidase（MPO）ELISAKit.PurposeThiskitallowsforthedeterminationofMPOconcentrationsinRatserum,bloodplasma,andotherbiologicalfluids.PrincipleoftheassayThekitassayRatMPOlevelinthesample,usePurifiedRatMPOantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddMPOtowells,CombinedMPOantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofMPOinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.MaterialsprovidedwiththekitMaterialsprovidedwiththekit48determinations96determinationsStorageUsermanual11Closureplatemembrane22Sealedbags11Microelisastripplate112-8℃Standard：180U/L0.5ml×1bottle0.5ml×1bottle2-8℃Standarddiluent1.5ml×1bottle1.5ml×1bottle2-8℃HRP-Conjugatereagent3ml×1bottle6ml×1bottle2-8℃Samplediluent3ml×1bottle6ml×1bottle2-8℃ChromogenSolutionA3ml×1bottle6ml×1bottle2-8℃ChromogenSolutionB3ml×1bottle6ml×1bottle2-8℃StopSolution3ml×1bottle6ml×1bottle2-8℃washsolution（20ml×20fold）×1bottle（20ml×30fold）×1bottle2-8℃Specimenrequirements1.serum-coagulationatroomtemperature10-20mins，centrifugation20-minatthespeedof2000-3000r.p.m.removesupernatant,Ifprecipitationappeared,Centrifugalagain.2.plasma-usesuitedEDTAorcitrateorasananticoagulant,mix10-20mins,centrifugation20-minatthespeedof2000-3000r.p.m.removesupernatant,Ifprecipitationappeared,Centrifugalagain.3.Urine-collectsueasterilecontainer,centrifugation20-minatthespeedof2000-3000r.p.m.removesupernatant,Ifprecipitationappeared,Centrifugalagain.TheOperationofHydrothoraxandcerebrospinalfluidReferencetoit.4.cellculturesupernatant-detectsecretorycomponents,collectsueasterilecontainer,centrifugation20-minatthespeedof2000-3000r.p.m.removesupernatant,detectthecompositionofcells,DilutcellsuspensionwithPBS（PH7.2-7.4）,Cellconcentrationreached1million/ml,repeatedfreeze-thawcycles,damagecellsandreleaseofintracellularcomponents,centrifugation20-minatthespeedof2000-3000r.p.m.removesupernatant,Ifprecipitationappeared,Centrifugalagain.5.Tissuesamples-Aftercuttingsamples,checktheweight,addPBS（PH7.2-7.4）,Rapidlyfrozenwithliquidnitrogen,maintainsamplesat2-8℃aftermelting,addPBS（PH7.4）,HomogenizedbyhandorGrinders,centrifugation20-minatthespeedof2000-3000r.p.m.removesupernatant.6.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.7.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.Assayprocedure1.DiluteandaddsampletoStandard:set10StandardwellsontheELISAplatescoated,addStandard100μltothefirstandthesecondwell,thenaddStandarddilution50μltothefirstandthesecondwell,mix;takeout100μlformthefirstandthesecondwellthenaddittothethirdandtheforthwellseparately.thenaddStandarddilution50μltothethirdandtheforthwell,mix;thentakeout50μlfromthethirdandtheforthwelldiscard,add50μltothefifthandthesixthwell,thenaddStandarddilution50μltothefifthandthesixthwell,mix;takeout50μlfromthefifthandthesixthwellandaddtotheseventhandtheeighthwell,thenaddStandarddilution50μltotheseventhandtheeighthwell,mix;takeout50μlfromtheseventhandtheeighthwellandaddtotheninthandthetenthwell,addStandarddilution50μltotheninthandthetenthwell,mix,takeout50μlfromtheninthandthetenthwelldiscard（addSample50μltoeachwellafterDiluting,（density:120U/L,80U/L,40U/L,20U/L,10U/L）2.addsample：Setblankwellsseparately（blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame）.testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl（samplefinaldilutionis5-fold）,addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-fold（or20-fold）washsolutiondiluted30-fold（or20-fold）withdistilledwaterandreserve.5.washing：UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.addenzyme：AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.incubate：Operationwith3.8.washing：Operationwith5.9.color：AddChromogenSolutionA50ulandChromogenSolutionBtoeachwell,evadethelightpreservationfor15minat37℃10.Stopthereaction：AddStopSolution50μltoeachwell,Stopthereaction（thebluecolorchangetoyellowcolor）.11.assay：takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.Importantnotes1.Thekittakesoutfromtherefrigerationenvironment首ldbebalanced15-30minutesintheroomtemperature,ELISAplatescoatedifhasnotuseupafteropened,theplate首ldbestoredinSealedbag.2.washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.3.addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.4.ifthetestingmaterialcontentisexcessivelyhigher（ThesampleODisbiggerthanthefirststandardwell）,pleasediluteSample（n-fold）,Pleasediluenteandmultipliedbythedilutionfactor.（×n×5）.5.Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.6.Thesubstrateevadethelightpreservation.7.Pleaseaccordingtouseinstructionstrictly,Thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.8.Allsamples,washingbufferandeachkindofreject首ldaccordingtoinfectivematerialprocess.9.Donotmixreagentswiththosefromotherlots.Takethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.CalculateThischartisforreferenceonlyAssayrange8U/L-150U/LStorageandvalidity1．Storage：2-8℃.2．validity：sixmonths.[详细]
2018-10-23 10:31
产品样册
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Goat Anti-Pig Interleukin 6

GoatAnti-PigInterleukin6Storage:2-8°CPackagesize:96determinationsPRINCIPLEOFTHEMETHODTheIL-6kitisasolidphasephasesandwichenzymelinkedimmunosorbentassay(ELISA).Samples,includingstandardsofknownIL-6concentrationsandunknownsarepipettedintothesewells.Duringthefirstincubation,theIL-6antigenandabiotinylatedmonoclonalantibodyspecificforIL-6aresimultaneouslyincubated.Afterwashing,theenzyme(streptavidin-peroxydase)isadded.Afterincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionwhichisactingontheboundenzymeisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIL-6presentinthesamples.REAGENTSPROVIDEDANDRECONSTTTUTIONREAGENTS（Storeat2-8℃）1×96WELLS0.5×96WELLSRECONSTTTUTION96/48-wellsmicrotiterplates10.5Ready-to-usePlastivcover21Ready-to-useStandard:800pg/ml1Vials(0.6ml)0.5Vials(0.3ml)Seereagentspreparationonpage3Blankcontrol1Vials(1.0ml)1Vials(0.5ml)Ready-to-useStandardDiluent1Vials(4.0ml)1Vials(2.0ml)Ready-to-useBiotinylatedanti-IL-61Vials(6.0ml)1Vials(3.0ml)Ready-to-useStreptavidin-HRP1Vials(8.0ml)1Vials(4.0ml)Ready-to-useWashingBuffer1Vials(20ml)1Vials(10ml)50×concentrateSubstrateA1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSubstrateB1Vials（6.0ml)1Vials(3.0ml)Ready-to-useStoppingSolution1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSampleDiluent1Vials(12ml)1Vials(6.0ml)Ready-to-useMATERIALREQUIREDBUTNOTPROVIDED?Distilledwater?Pipettes:10ul、50ul、100ul、200ul、1000ul。?Vortexmixerandmagneticstirrer.SAFETY?Forresearchuseonly?AvoidanyskincontactwithH2SO4andTMB.Incaseofcontact,washthoroughlywater.?Donoteat,drink,smokeorapplycosmeticswherekitreagentsareused.?Donotpipettebymouth.PROCEDURALNOTES/LAB.QUALITYCONTROL?Whennotinuse,kitcomponents首ldbestoredrefrigeratedorfrozenasindicatedonvialsorbottles.Allreagents首ldbewarmedtoroomtemperaturebeforeuse.Lyophilizedstandards首ldbediscardedafteruse.?Oncethedesirednumberofstripshasbeenremoved,immediatelyresealthebagtoprotecttheremainingstripsfromedterioration.?Coverorcapallreagentswhennotinuse.?Donotmisorinterchangereagentsbetweendifferentlots.?Donotusereagentsbeyondtheexpirationdateofthekit.?Useacleandisposableplasticpipettetipforeachreagent,standard,orspecimenadditioninordertoavoidcross-contamination,forthedispensingofH2SO4andsubstratesolution,avoidpipetteswithmetalparts.?Useacleanplasticcontainertopreparethewashingsolution.?Thoroughlymixthereagentsandsamplesbeforeusebyagitationorswir领.?Allresidualwashingliquidmustbedrainedfromthewellsbyefficientaspirationorbydecantationfollowedbytappingtheplateforcefullyonabsorbentpaper.Neverinsertabsorbentpaperdirectlyintothewells.?TheTMBsolutionislightsensitive.Avoidprolongedexposuretolight,also,avoidcontactoftheTMBsolutionwithmetaltopreventcolourdevelopment.WarningTMBistoxicavoiddirectcontactwithhands.Disposeoffproperly.Ifadarkbluecolourdevelopswithinafewminutesafterpreparation,thisindicatesthattheTMBsolutionhasbeencontaminatedandmustbediscarede.Readabsorbanceswithin1houraftercompletionoftheassay.?Whenpipettingreagents,maintainaconsistentorderofadditionfromwell-to-well.Thiswillensureequalincubationtimesforallwells.?Respectincubationtimesdescribedintheassayprocedure.SPECIMENCOLLECTION\PROCESSINGANDSTORAGE?Serum---Avoidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serum首ldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.?Plasma---EDTA\citrateandheparinplasmacanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestplasma.?Cellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.?Storage---Ifnotanalyzedshortlyaftercollection,samples首ldbealiquoted(250-500ul)toavoidfreeze-thawcyclesandstoredfrozenat-70℃.Avoidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,avoiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,this首ldberemovedpriortoassaybycentrifugationorfiltration.?Recommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbeforeassaying.PREPARATIONOFREAGENTS?Standards:Standardhavetobereconstituledwiththevolumeofstandardbufferdiluentindicatedonthevial.Thisreconstitutionproducesastocksolutionof800pg/mlIL-6.Allowstandardtostandfor5?minuteswithgentleswir领priortomakingdilutions.Serialdilutionsofstandardmustbemadebeforeeachassysandcannotbestored.800pg/ml（6Standard）Originaldensity50ul。400pg/ml（5Standard）100ul6Standard+100uldiludent200pg/ml（4Standard）100ul5Standard+100uldiludent100pg/ml（3Standard）100ul4Standard+100uldiludent50pg/ml（2Standard）100ul3Standard+100uldiludent25pg/ml（1Standard）100ul2Standard+100uldiludent0pg/mlBlankControl50ul。?Washingbuffer50×concentrate:Dilute50timesindistilledwater.ASSAYMETHOD?Beforeuse,mixallreagentsthoroughlywithoutmakingfoam.?Determinethenumberofmicrowellstripsrequiredtotestthedesirednumberofsamples,plusappropriatenumberofwellsneededforrunningblanksstandards.Eachsample,standardandblank首ldbeassayedinduplicate.Removesufficientmicrowellstripsfromthepouch.?Add50ulofstandarddiluenttostandardwellsB1,B2,C1,C2,D1,D2,E1,E2,F1,F2.Reconstitutestandardvialwiththeappropriatevolumeasdescribedinthechapterreagentspreparation.Preparation.Pipet100ulofstandardintowellsA1andA2(seeplateschemebelow).Transfer50ulfromA1andA2toB1andB2wells.Mixthecontentsbyrepeatedaspirationsandejections.Takecarenottoscratchtheinnersurfaceofmicrowells.RepatthisprocedurefromthewellsB1,B2towellsC1,C2andfromwellsC1,C2toD1,D2andsooncreatingtwoparallelrowsofIL-6standarddilutionsranging，Add50ulofstandarddiluenttotheblandwells.?Dilutesamples1:1distribing50ulofsampleinto50ulofdilluent,Add50ulofdilutedsampletowells..?Add50ulofdilutedbiotinylatedanti-IL-6toallwells.?Coverwithaplatevoverandincubatefor1hourat37℃.?Removethecoverandwashtheplateasfollows:⑴aspiratetheliquidfromeachwell,⑵dispensse0.3mlofwashingsolutionintoeachwell.⑶Aspirateagainthecontetofeachwellafter0.5minute.⑷Repeatsteps⑵and⑶threetimes.?Distribute60ulofstreptavidin-HRPsolutiontoallwells,includingblankwells.?Coverandincubate30minat37℃.?Removethecoverandemptywells,Washmicrowellstripsaccordingtostep,Proceedimmediatelytothenextstep.?Add50ulSubstrateAandSubstrateBtoeachwell。Incubatefor10minat37℃。?Theenzyme-substratereactionisstoppedbyquicklypipetting50ulofH2SO4.stopreagentintoeachwell,includingtheblankwells,tocompletelyanduniformlyinactivatetheenzyme.ResultsmustberedimmediatelyaftertheadditionofH2SO4.?Readabsorbanceofeachwellonaspectrophotometerusing450nmastheprimarywavelengthandoptionally620nm(610nmto650nmisacceptable)asthereferencewavelength.SUGGESTEDPLATESCHEMEStandardconcentrations（pg/ml）A800800samplesamplesamplesamplesamplesamplesamplesamplesamplesampleB400400samplesamplesamplesamplesamplesamplesamplesamplesamplesampleC200200samplesamplesamplesamplesamplesamplesamplesamplesamplesampleD100100samplesamplesamplesamplesamplesamplesamplesamplesamplesampleE5050samplesamplesamplesamplesamplesamplesamplesamplesamplesampleF2525samplesamplesamplesamplesamplesamplesamplesamplesamplesampleG00samplesamplesamplesamplesamplesamplesamplesamplesamplesampleHsamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesampleLIMITATIONSOFTHEPROCEDUREDonotextrapolatethestandardcurvebeyondthemaxstandardcurvepoint.Thedose-responseisnon-linearinthisregionandgoodaccruacyisdifficulttoobtain.CALCULATIONOFRESULTSTheminimumdetectableconcentrationinthisassayisestimatedtobe1.0pg/ml[详细]
2018-09-27 10:00
产品样册
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Goat interleukin 17(IL-17)

Goat interleukin 17(IL-17)[详细]
2024-09-28 01:06
课件
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Goat Anti-Rabbit Motilin Receptor

Goat Anti-Rabbit Motilin Receptor[详细]
2024-09-28 10:33
其它
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Anti-CD4 antibody [mAb51312]抗体说明书

ProductoverviewDescriptionMousemonoclonal[mAb51312]toCD4HostspeciesMouseTestedapplicationsIHC-P,WB,IHC-FrCrossreactivityReactswithMouse,HumanImmunogenSyntheticpeptideconjugatedtoKLHderivedfromwithinresidues50-150ofHumanCD4.（Note:theaminoacidsequenceisproprietary）（Peptideavailableasab54699.）PositivecontrolThisantibodygaveapositivesignalinHumanThymusandHumanLymphNodeTissueLysateTargetFunctionAccessoryproteinforMHCclass-IIantigen/T-cellreceptorinteraction.MayregulateT-cellactivation.Inducestheaggregationoflipidrafts.SequencesimilaritiesContains3Ig-likeC2-type（immunoglobulin-like）domains.Contains1Ig-likeV-type（immunoglobulin-like）domain.Post-translationalmodificationsPalmitoylationandassociationwithLCKcontributetotheenrichmentofCD4inlipidrafts.CellularlocalizationCellmembrane.Localizestolipidrafts.RemovedfromplasmamembranebyHIV-1Nefproteinthatincreasesclathrin-dependentendocytosisofthisantigentotargetittolysosomaldegradation.Cellsurfaceexpressionisalsodown-modulatedbyHIV-1Envelopepolyproteingp160thatinteractswith,andsequestersCD4intheendoplasmicreticulum.Targetinformationabovefrom:UniProtaccessionP01730TheUniProtConsortiumTheUniversalProteinResource（UniProt）in2010NucleicAcidsRes.38:D142-D148（2010）.AlternativenamesCD4antibodyCD4（L3T4）antibodyCD4antibodyCD4antibodyCD4antigen（p55）antibodyCD4AntigenantibodyCD4moleculeantibodyCD4ReceptorantibodyCD4+Lymphocytedeficiency,includedantibodyCD4_HUMANantibodyCD4mutantibodyL3T4antibodyLeu3antibodyLy-4antibodyLymphocyteantigenCD4antibodyMGC165891antibodyOTTHUMP00000238897antibodyp55antibodyTCellAntigenT4antibodyTcellantigenT4/LEU3antibodyTcelldifferentiationantigenL3T4antibodyTcellOKT4deficiency,includedantibodyhttp://www.abcam.com/CD4-antibody-mAb51312-ab51312.htmlUKoffice:Abcamplc330CambridgeScienceParkCambridgeCB40FLUKTel:+44（0）1223696000Fax:+44（0）1223215215Email:orders@abcam.comUSoffice:AbcamInc.1KendallSquare,SuiteB2304Cambridge,MA02139-1517USATel:（888）77-ABCAM（22226）Fax:（877）774-8286ThesenumbersaretollfreeintheUS/CanadaEmail:us.orders@abcam.comLastupdatedonAugust24,2012TcellsurfaceantigenT4/Leu3antibodyTcellsurfaceantigenT4/Leu3antibodyTCellSurfaceAntigenT4/Leu3antibodyTCellSurfaceGlycoproteinCD4antibodyT-cellsurfaceantigenT4/Leu-3antibodyT-cellsurfaceglycoproteinCD4antibodyW3/25antibodyW3/25antigenantibodyPropertiesFormLiquidStorageinstructionsStoreat+4°Cshortterm（1-2weeks）.Aliquotandstoreat-20°Cor-80°C.Avoidrepeatedfreeze/thawcycles.StoragebufferPreservative:0.02%SodiumAzideConstituents:PBS,pH7.4SeethewebsiteformoreSDSinformationforthisproduct.PurityProteinGpurifiedClonalityMonoclonalClonenumbermAb51312IsotypeIgG1LightchaintypekappaApplicationsIHC-PIHC-P:1/50.WBWB:Useaconcentrationof1-5μg/ml.Detectsabandofapproximately55kDa（predictedmolecularweight:51kDa）.IHC-FrIHC-Fr:Useatanassaydependentconcentration.PubMed:21690251Images（Seethewebsiteforhigherresolutionimagesofthisproduct）OurAbpromisetoyou:QualityguaranteedandexperttechnicalsupportIftheproductdoesnotperformasdescribedonthisdatasheet,wewillofferarefundorreplacement.ForfulldetailsoftheAbpromise,pleasevisithttp://www.abcam.com/abpromiseorcontactourtechnicalteam.[详细]
2018-10-01 10:01
产品样册
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Covalently coup领 the antibody on an amine-self-as

Covalently coup领 the antibody on an amine-self-as[详细]
2024-09-17 19:14
其它
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Rat LPS试剂盒

RatLPS试剂盒RatLPSFORRESEARCHUSEONLYAssayrange：0.03Eu/L0.8Eu/L96determinationsPurposeThiskitallowsforthedeterminationofLPSconcentrationsinRatserum,cellculturesupernatesandotherbiologicalfluidsRatLPS试剂盒PrincipleoftheassayThekitassayRatLPSlevelinthesample,usePurifiedRatLPSantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddLPStowells,CombinedLPSantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofRatLPSinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.RatLPS试剂盒Materialsprovidedwiththekit1washsolution20ml×1bottle7StopSolution6ml×1bottle2HRP-Conjugatereagent6ml×1bottle8Standard（1.6Eu/L）0.5ml×1bottle3Microelisastripplate12well×8strips9Standarddiluent1.5ml×1bottle4Samplediluent6ml×1bottle10Instruction15ChromogenSolutionA6ml×1bottle11ClosureplateRatLPS试剂盒membrane26ChromogenSolutionB6ml×1bottle12Sealedbags1Specimenrequirements1.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevant2literature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.2.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.Assayprocedure1.Diluteandaddsample:DiluteOriginaldensityStandardasfollowtable:0.8Eu/L5Standard150μlOriginaldensityStandard+150μlStandarddiluent0.4Eu/L4Standard150μl5Standard+150μlStandarddiluent0.2Eu/L3Standard150μl4Standard+150μlStandarddiluent0.1Eu/L2Standard150μl3Standard+150μlStandarddiluent0.05Eu/L1Standard150μl2Standard+150μlStandarddiluent2.addsample：Setblankwellsseparately(blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame).testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl(samplefinaldilutionis5-fold),addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-fold(or20-fold)washsolutiondiluted30-fold(or20-fold)withdistilledwaterandreserve.5.washing：UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.addenzyme：AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.incubate：Operationwith3.8.washing：Operationwith5.9.color：AddChromogenSolutionA50ulandChromogenSolutionBtoeachwell,evadethelightpreservationfor15minat37℃10.Stopthereaction：AddStopSolution50μltoeachwell,Stopthereaction(thebluecolorchangetoyellowcolor).11.assay：takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.RatLPS试剂盒3StepsdescriptionStandard,SamplediluentAddStandard,Samplediluent,incubatefor30minat37℃.Wash5time,AddHRP-Conjugatereagent,incubatefor30minat37℃.Wash5times,AddChromogenSolutionAandB,incubatefor30minat37℃.AddStoppSolutionReadabsorbanceat450nmwithin15mincalculateCalculateTakethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthe4sampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.Importantnotes1.Thekittakesoutfromtherefrigerationenvironment首ldbebalanced15-30minutesintheroomtemperature,ELISAplatescoatedifhasnotuseupafteropened,theplate首ldbestoredinSealedbag.2.washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.3.addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.4.ifthetestingmaterialcontentisexcessivelyhigher(ThesampleODisbiggerthanthefirststandardwell),pleasediluteSample(n-fold),Pleasediluenteandmultipliedbythedilutionfactor.（×n×5）.5.Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.6.Thesubstrateevadethelightpreservation.7.Pleaseaccordingtouseinstructionstrictly,Thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.8.Allsamples,washingbufferandeachkindofreject首ldaccordingtoinfectivematerialprocess.9.Donotmixreagentswiththosefromotherlots.RatLPS试剂盒Storageandvalidity1．Storage：2-8℃.2．validity：sixmonths[详细]
2018-10-31 10:00
产品样册
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Goat Anti-Dog Tumor necrosis factor

Goat Anti-Dog Tumor necrosis factor [详细]
2014-04-22 00:00
安装说明
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Goat Anti-Mouse IgG-HRP 二抗

GoatAnti-MouseIgG-HRPabmart艾比玛特二抗上海桥星生物销售直线：021-65672052（蒋先生）咨询电话：18221794820客服QQ：1468746680上海桥星产品仅供科研，请勿挪作他用。产品名称：GoatAnti-MouseIgG-HRP品牌：abmart艾比玛特货号：M21001规格：S-100μl应用范围：WB,ELISA,DotBlot相关产品：ABMART二抗/Beads抗体CatalogResearchareaAntibody抗体Isotype同种型Specificity专一性Application用途Catalog目录Package规格Price定价二抗GoatAnti-MouseIgG-HRPGoat-WB,ELISA,DotBlotM21001S-100μl120L-1ml900GoatAnti-Rabbit&MouseIgG-HRPGoat-WB,ELISA,DotBlotM21003S-100μl120L-1ml900GoatAnti-RabbitIgG-HRPGoat-WB,ELISA,DotBlotM21002S-100μl120L-1ml900Beads抗体Anti-Myc-TagmAb(Agaroseconjugated)MouseAllIPM20012S-500μl2,200L-5ml9000Anti-HA-TagmAb(Agaroseconjugated)MouseAllIPM20013S-500μl2,200L-5ml9000Anti-DYKDDDDK-TagmAb(Agaroseconjugated)(SameasSigma'sAnti-FLAG)MouseAllIPM20018S-500μl2200L-5ml9000ReactivityKey:Hhuman,Mmouse,Rrat,Mkmonkey,DmD.melanogaster,DgDog,ChHmChinesehamster,AllallspeciesexpectedGoatAnti-MouseIgG-HRPabmart艾比玛特二抗上海桥星生物客服电话：021-65672052手机：18221794820公司简介：上海桥星生物提供优质的分子生物学试剂和试剂盒、美国联合碳化和美国光谱医学透析袋、培养基和培养耗材、细胞因子和细胞分离液、抗体和Elisa试剂盒及常用生物试剂等，Sigma、Amresco等各大量现货火爆促销中，欢迎登录www.bridge-star.com或拨打021-65672052、18221794820[详细]
2019-01-01 10:01
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Rat Aβ1-42 ELISA kit

www.biokanu.comRatamyloidbetapeptide1-42（Aβ1-42）ELISAKitProd.No.3R285FROM:RBForthequantitativeinvitrodeterminationofAβ1-42concentrationsinRatsupernates,serum,plasmaandtissue.FORRESEARCHUSEONLY.NOTFORUSEINDIAGNOSTICPROCEDURES.TABLEOFCONTENTSContentsPageTABLEOFCONTENTS..2INTENDEDUSE..3PRINCIPLE..3WARNINGSANDPRECAUTIONS..4MATERIALSPROVIDEDWITHTHEKIT.7MATERIALSREQUIREDBUTNOTPROVIDED..7STORAGECONDITIONS..8REAGENTPREPARATION..9SPECIMENCOLLECTIONANDPREPARATION..9ASSAYPROCEDURE..10CALCULATIONOFRESULTS..13REFERENCES..14INTENDEDUSEAnenzymeimmunoassayforthequantitativeinvitrodiagnosticmeasurementofRatAβ1-42incellculturesupernates,serum,plasmaandtissue.PRINCIPLEThekitassayRatAβ1-42levelinthesample，usePurifiedRatAβ1-42antibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddAβ1-42towells,CombinedAβ1-42antibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofAβ1-42inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.WARNINGSANDPRECAUTIONSlThiskitisforinvitrodiagnosticuseonly.Forprofessionaluseonly.lAllreagentsofthistestkitwhichcontainhumanserumorplasmahavebeentestedandconfirmednegativeforHIVI/II,HBsAgandHCVbyFDAapprovedprocedures.Allreagents,however,首ldbetreatedaspotentialbiohazardsinuseandfordisposal.lBeforestartingtheassay,readtheinstructionscompletelyandcarefully.Usethevalidversionofthepackageinsertprovidedwiththekit.Besurethateverythingisunderstood.lThemicroplatecontainssnap-offstrips.Unusedwellsmustbestoredat2°Cto8°Cinthesealedfoilpouchandusedintheframeprovided.lPipettingofsamplesandreagentsmustbedoneasquicklyaspossibleandinthesamesequenceforeachstep.lUsereservoirsonlyforsinglereagents.Thisespeciallyappliestothesubstratereservoirs.Usingareservoirfordispensingasubstratesolutionthathadpreviouslybeenusedfortheconjugatesolutionmayturnsolutioncolored.Donotpourreagentsbackintovialsasreagentcontaminationmayoccur.lMixthecontentsofthemicroplatewellsthoroughlytoensuregoodtestresults.Donotreusemicrowells.lDonotletwellsdryduringassay;addreagentsimmediatelyaftercompletingtherinsingsteps.lAllowthereagentstoreachroomtemperature（21-26°C）beforestartingthetest.Temperaturewillaffecttheabsorbancereadingsoftheassay.However,valuesforthepatientsampleswillnotbeaffected.lNeverpipetbymouthandavoidcontactofreagentsandspecimenswithskinandmucousmembranes.lDonotsmoke,eat,drinkorapplycosmeticsinareaswherespecimensorkitreagentsarehandled.lWeardisposablelatexgloveswhenhand领specimensandreagents.Microbialcontaminationofreagentsorspecimensmaygivefalseresults.lHand领首ldbedoneinaccordancewiththeproceduresdefinedbyanappropriatenationalbiohazardsafetyguidelineorregulation.lDonotusereagentsbeyondexpirydateasshownonthekitlabels.lAllindicatedvolumeshavetobeperformedaccordingtotheprotocol.Optimaltestresultsareonlyobtainedwhenusingcalibratedpipettesandmicrotiterplatereaders.lDonotmixorusecomponentsfromkitswithdifferentlotnumbers.Itisadvisednottoexchangewellsofdifferentplatesevenofthesamelot.Thekitsmayhavebeenshippedorstoredunderdifferentconditionsandthebindingcharacteristicsoftheplatesmayresultslightlydifferent.lAvoidcontactwithStopSolutioncontaining0.5MH2SO4.Itmaycauseskinirritationandburns.lSomereagentscontainProclin,BNDand/orMITaspreservatives.Incaseofcontactwitheyesorskin,flushimmediatelywithwater.lTMBsubstratehasanirritanteffectonskinandmucosa.Incaseofpossiblecontact,washeyeswithanabundantvolumeofwaterandskinwithsoapandabundantwater.Washcontaminatedobjectsbeforereusingthem.Ifinhaled,takethepersontoopenair.lChemicalsandpreparedorusedreagentshavetobetreatedashazardouswasteaccordingtothenationalbiohazardsafetyguidelineorregulation.lForinformationonhazardoussubstancesincludedinthekitpleaserefertoMaterialSafetyDataSheetsMATERIALSPROVIDEDWITHTHEKITMaterialsprovidedwiththekit96determinationsStorageUsermanual1Closureplatemembrane2Sealedbags1Microelisastripplate12-8℃Standard：900pg/ml0.5ml×1bottle2-8℃Standarddiluent1.5ml×1bottle2-8℃HRP-Conjugatereagent6ml×1bottle2-8℃Samplediluent6ml×1bottle2-8℃ChromogenSolutionA6ml×1bottle2-8℃ChromogenSolutionB6ml×1bottle2-8℃StopSolution6ml×1bottle2-8℃washsolution30×20ml×1bottle2-8℃MATERIALSREQUIREDBUTNOTPROVIDEDlMicroplatereadercapableofmeasuringabsorbanceat450nm.lPrecisionpipettestodeliver2mlto1mlvolumes.l100mland1litergraduatedcylinders.lCalibratedadjustableprecisionpipettes,preferablywithdisposableplastictips.（Amanifoldmulti-channelpipetteisdesirableforlargeassays.）lAbsorbentpaper.l37°Cincubator.lDistilledordeionizedwater.lDataanalysisandgraphingsoftware.Graphpaper:linear（Cartesian）,log-logorsemi-log,orlog-logitasdesired.lTubestopreparestandardorsampledilutions.STORAGECONDITIONSuWhenstoredat2°Cto8°Cunopenedreagentswillretainreactivityuntilexpirationdate.uDonotusereagentsbeyondthisdate.Openedreagentsmustbestoredat2°Cto8°C.uMicrotiterwellsmustbestoredat2°Cto8°C.Oncethefoilbaghasbeenopened,care首ldbetakentocloseittightlyagain.uOpenedkitsretainactivityfor8weeksifstoredasdescribedabove.REAGENTPREPARATIONBringallreagentstoroomtemperaturebeforeuseSPECIMENCOLLECTIONANDPREPARATIONSerum-Useaserumseparatortube（SST）andallowsamplestoclotfor30minutesbeforecentrifugationfor15minutesatapproximately1000xg.Removeserumandassayimmediatelyoraliquotandstoresamplesat-20°Cor-80°C.Plasma-CollectplasmausingEDTAorheparinasananticoagulant.Centrifugesamplesfor15minutesat1000xgat2-8°Cwithin30minutesofcollection.Storesamplesat-20°Cor-80°C.Avoidrepeatedfreeze-thawcycles.Cellculturefluidandotherbiologicalfluids-Removeparticulatesbycentrifugationandassayimmediatelyoraliquotandstoresamplesat-20°Cor-80°C.Avoidrepeatedfreeze-thawcyclesASSAYPROCEDUREuGeneralRemarkslAllreagentsandspecimensmustbeallowedtocometoroomtemperaturebeforeuse.Allreagentsmustbemixedwithoutfoaming.lOncethetesthasbeenstarted,allsteps首ldbecompletedwithoutinterruption.lUsenewdisposalplasticpipettetipsforeachstandard,controlorsampleinordertoavoidcrosscontamination.lAbsorbanceisafunctionoftheincubationtimeandtemperature.Beforestartingtheassay,itisrecommendedthatallreagentsareready,capsremoved,allneededwellssecuredinholder,etc.Thiswillensureequalelapsedtimeforeachpipettingstepwithoutinterruption.lAsageneralruletheenzymaticreactionislinearlyproportionaltotimeandtemperature.lDetermineabsorptionwithanELISAreaderat450nmagainst620nmasreference.Ifnoreferencewavelengthisavailable,readonlyat450nm.Iftheextinctionofthehigheststandardexceedsthemeasurementrangeofthephotometer,absorptionmustbemeasuredimmediatelyat405nmagainst620nmasreference.uAssayProcedure1.DiluteandaddsampletoStandard:set10StandardwellsontheELISAplatescoated,addStandard100μltothefirstandthesecondwell,thenaddStandarddilution50μltothefirstandthesecondwell,mix;takeout100μlformthefirstandthesecondwellthenaddittothethirdandtheforthwellseparately.thenaddStandarddilution50μltothethirdandtheforthwell,mix;thentakeout50μlfromthethirdandtheforthwelldiscard,add50μltothefifthandthesixthwell,thenaddStandarddilution50μltothefifthandthesixthwell,mix;takeout50μlfromthefifthandthesixthwellandaddtotheseventhandtheeighthwell,thenaddStandarddilution50μltotheseventhandtheeighthwell,mix;takeout50μlfromtheseventhandtheeighthwellandaddtotheninthandthetenthwell,addStandarddilution50μltotheninthandthetenthwell,mix,takeout50μlfromtheninthandthetenthwelldiscard（addSample50μltoeachwellafterDiluting,（density:600pg/ml，400pg/ml，200pg/ml,100pg/ml，50pg/ml）.50pg/ml100pg/ml600pg/ml200pg/ml900pg/ml400pg/ml2.addsample：Setblankwellsseparately（blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame）.testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl（samplefinaldilutionis5-fold）,addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-foldwashsolutiondiluted30-foldwithdistilledwaterandreserve.5.washing：UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.addenzyme：AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.incubate：Operationwith3.8.washing：Operationwith5.9.color：AddChromogenSolutionA50ulandChromogenSolutionBtoeachwell,evadethelightpreservationfor15minat37℃10.Stopthereaction：AddStopSolution50μltoeachwell,Stopthereaction（thebluecolorchangetoyellowcolor）.11.assay：takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.CALCULATIONOFRESULTSlCalculatetheaverageabsorbancevaluesforeachsetofstandards,controlsandpatientsamples.lConstructastandardcurvebyplottingthemeanabsorbanceobtainedfromeachstandardagainstits.lconcentrationwithabsorbancevalueonthevertical（Y）axisandconcentrationonthehorizontal（X）axis.lUsingthemeanabsorbancevalueforeachsampledeterminethecorrespondingconcentrationfromthestandardcurve.lAutomatedmethod:TheresultsintheIFUhavebeencalculatedautomaticallyusinga4PL.l（4ParameterLogistics）curvefit.4ParameterLogisticsisthepreferredcalculationmethod.Otherdata.lreductionfunctionsmaygiveslightlydifferentresults.lTheconcentrationofthesamplescanbereaddirectlyfromthisstandardcurve.Sampleswith.lconcentrationshigherthanthatofthehigheststandardhavetobefurtherdiluted.Forthecalculationof.ltheconcentrationsthisdilutionfactorhastobetakenintoaccount.REFERENCESREF:Cat.-No.:/Kat.-Nr.:/No.-Cat.:/Cat.-No.:/N.Cat.:/N.CatLOT:Lot-No.:/Chargen-Bez.:/No.Lot:/Lot-No.:/LoteN.:/Lotton.::No.ofTests:/Kitgre:/Nb.deTests:/No.deDeterm.:/N.deTestes:/Quantitàdeitests::Keepawayfromheatordirectsunlight./VorHitzeunddirekterSonneneinstrahlungschützen./Garderàl’abridelachaleuretdetouteexpositionlumineuse./Manténgasealejadodelcalorolaluzsolardirecta./Manterlongedocalorouluzsolardirecta./Nonesporreairaggisolari.:Readinstructionsbeforeuse./Arbeitsanleitunglesen./Lirelafichetechniqueavantemploi./Lealasinstruccionesantesdeusar./Lerasinstruesantesdeusar./Leggereleistruzioniprimadell’uso.:Storeat:/Lagernbei:/Stockerà:/Almacenea:/Armazenara:/Conservarea:[详细]
2018-09-22 10:00
产品样册
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该会员其他资料: 小鼠红细胞生成素(EPO)检测试剂盒; 小鼠嗜酸粒细胞趋化蛋白Eotaxin 1(Eotaxin 1/CCL11)检测试剂盒; 小鼠嗜酸粒细胞趋化因子(ECF)检测试剂盒; 小鼠可溶性血管内皮细胞蛋白C受体(sEPCR)检测试剂盒; 小鼠主要组织相容性复合体Ⅱ类(MHC-Ⅱ/H-2Ⅱ）检测试剂盒; 小鼠CD30分子(CD30)检测试剂盒; 小鼠E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)检测试剂盒; 小鼠主要组织相容性复合体Ⅲ类(MHC-Ⅲ/H-2Ⅲ)检测试剂盒; 小鼠载脂蛋白H(Apo-H)检测试剂盒; 小鼠白介素7(IL-7)检测试剂盒

Rat Anti- Goat Brucella Antibody

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