This Application Note presents different online 2D-LC concepts for the high-resolution analysis of complex N-linked glycans in erythropoietin (EPO) using the Agilent 1290 Infinity 2D-LC Solution with fluorescence detection. EPO has a complex glycosylation pattern with differently branched and charged glycans. A combination of hydrophilic interaction chromatography (HILIC) with weak anion exchange chromatography (WAX) enables highly orthogonal separation. Comprehensive 2D-LC analysis with HILIC in the first and WAX in the second dimension facilitates high-resolution 2D chromatography together with simultaneous charge profi领. In addition, multiple heart-cutting 2D-LC analysis, combining WAX and HILIC separation, provides a flexible alternative whereby the user can select multiple peaks to be analyzed in the second dimension and, moreover, run longer gradients.
This Application Note presents different online 2D-LC concepts for the high-resolution analysis of complex N-linked glycans in erythropoietin (EPO) using the Agilent 1290 Infinity 2D-LC Solution with fluorescence detection. EPO has a complex glycosylation pattern with differently branched and charged glycans. A combination of hydrophilic interaction chromatography (HILIC) with weak anion exchange chromatography (WAX) enables highly orthogonal separation. Comprehensive 2D-LC analysis with HILIC in the first and WAX in the second dimension facilitates high-resolution 2D chromatography together with simultaneous charge profi领. In addition, multiple heart-cutting 2D-LC analysis, combining WAX and HILIC separation, provides a flexible alternative whereby the user can select multiple peaks to be analyzed in the second dimension and, moreover, run longer gradients.[详细]
The analysis of impurities is of great importance during the development and production of pharmaceuticals and fi ne chemicals. This Application Note shows that multiple heart-cutting 2D-LC is ideally suited for method development for impurity analysis of pharmaceuticals and fi ne chemicals. [详细]
The Agilent 1290 Infinity II High Dynamic Range Diode Array Detection (HDR-DAD) Impurity Analyzer Solution combines the signals from two diode array detectors with different path length Max-Light cartridge cells to increase the linear dynamic UV-range. This solution can be used to detect and quantify main and trace compounds in a single run without exceeding the linear UV-range. Using one DAD with a 10-mm flow cell, typically two injections with different injection volumes are needed to determin[详细]
Over the last decades, an increasing number of generic drug formulations have been released on the pharmaceutical market. These products are generally analyzed with compendia methods.[详细]
The Agilent 1290 Infinity II LC was used to determine the bitter compounds iso-alpha-acids and reduced iso-alpha-acids, and the clove-like phenolic flavor 4-vinylguaiacol in bottled beer.[详细]
A preliminary assessment of environmental volatiles in water using static headspace analysis was performed using the Agilent 7697A Headspace Sampler, Agilent 7890B GC, and the Agilent 5977B GC/MSD system with high efficiency source (HES). The revolutionary design of the HES produces a higher ion currentyield for many compounds (greater sensitivity), which allows flexible approaches to sample analysis such as lowering detection limits, reducing sample size, speeding up analysis, and so forth.[详细]
Agilent Intelligent System Emulation Technology (ISET) within the Agilent 1290 Infinity II LC facilitates the transfer of methods from Agilent and non-Agilent HPLC and UHPLC systems. This Application Note shows how the 1290 Infinity II LC with ISET emulates the ternary
gradient of a Waters Acquity H-Class system for the analysis of peptides. Over 97 % agreement is achieved using the 1290 Infinity II LC with ISET, in contrast to without ISET.[详细]
The analytical requirements of the geological survey and mining industries are demanding in terms of high number of samples, number of elements, time/cost constraints and the difficult matrices. [详细]
Laser Ablation-ICP-MS (LA-ICP-MS) is used for the elemental analysis of solid samples and powders,including geological materials, ceramics, biological tissue and forensic samples.[详细]
Online 2D-LC Analysis of Complex N-Glycans in Biopharmaceuticals Using the Agilent 1290 Infinity 2D-LC Solution
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