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大鼠血小板因子3(PF-3)ELISA KitHSP-90(Mouse Heat Shock Protein 90) ELISA Kit
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大鼠血小板因子3(PF-3)ELISAKitHSP-90(MouseHeatShockProtein90)ELISAKit小鼠热休克蛋白90X1900396TMDAELISAKit大鼠丙二醛X1900496TSmad7(HumanMothersagainstdecapentaplegichomolog7)ELISAKit人Smad7X1900596TSODELISAKit大鼠超氧化物歧化酶X1900696TTAA(Humantumor-associatedantigen)ELISAKit人肿瘤相关抗原X1900796THSP-70(MouseHeatShockProtein70)ELISAKit小鼠热休克蛋白70X1900896TADP(Mouseadiponectin)ELISAKit小鼠脂联素X1900996THSPgp96ELISAKit大鼠血小板因子3(PF-3)ELISAKit大鼠热休克蛋白糖蛋白96X1901096TTIEG1(HumanTGF-beta-inducibleearlyresponsegene-1)ELISAKit人TGF-β诱导早期基因1X1901196T公司将ELISA试剂盒操纵中各个环节常泛起的问题我们总结一下:首先选择试剂:选择质量优良的检测试剂,严格按照试剂仿单进行操纵,操纵前将试剂在室温下平衡30-60分钟,试验以敏捷度较高、特异性较好的特点在科研上得到了广泛的应用,但操纵中的各个环节对试验的检测效果影响较大,如不留意,有可能导致显色不全、花板等结果,请避免反复冻融。特别要留意加样问题。加样:室温温育的试剂,特别是温育时间比较短的实验操纵的试剂,加样对数据的影响比较大。显色液(有A液和B液的),用前15分钟配制;尺度品:有的试剂盒中尺度品是已经配制好的,4度保留;有的是要现配制的;按照仿单操纵;浓缩生物素结合的二抗和HRP:假如需要配制,试剂盒没注明配好可以保留多久的话,尽量现配现用(用前15分钟配制);原则是ELISA试剂盒上没有指出配制物的蕴藏方式的话,应该现配现用;不要怕麻烦;还有小瓶的管子开盖前要离心,以免盖子上粘连以後总量不够。不好的操作原因:不会准确使用加样器;血清或血浆标天职离不好即进行加样;手工操纵中,加样板过多造成加样後放入孵箱前等待时间过长(特别是室内温度较高时);加完标本再加酶试剂时酶溅出孔外;参考预备实验的准确步骤,实验的事前预备工作,标本收集与保留:避免使用肉眼可见的溶血标本、高脂标本和混浊标本;2-8℃下,标本可放置24-48小时,长期保留需放置-20℃下。详细请参照:ELISA实验标本的采集与处理:ELISA实验标本的保留:试剂使用中的预备工作及留意事项:试剂盒中会有提示,一般需要预备的有:洗涤液;用前配制;有的试剂盒会标明,配好的4度下一般可以放一个月;假如没有标明,尽量用新鲜的,不要多配制。TAF(Humantumorangiogenesisfactors)ELISAKit人肿瘤血管生长因子X1901296THsp-40(MouseHeatShockProtein40)ELISAKit小鼠热休克蛋白40X1901396TCH50ELISAKit大鼠血清总补体X1901496TB7-2/sCD86ELISAKit大鼠可溶性CD86X1901596TCK-20(Humancytokeratin20)ELISAKit人细胞角蛋白20X1901696TABeta1-40(Mouseamyloidbetapeptide1-40)ELISAKit小鼠β淀粉样蛋白X1901796TCK-19(Humancytokeratin19)大鼠血小板因子3(PF-3)ELISAKitELISAKit人细胞角蛋白19X1901896TIL-8/CXCL8(Mouseinterleukin8)ELISAKit小鼠白介素8X1901996THIF-1AlphaELISAKit大鼠低氧诱导因子1αX1902096TAmylinELISAKit大鼠胰淀素X1902196TCyclin-D3(MouseCyclin-D3)ELISAKit小鼠细胞周期素D3X1902296TCK-18(Humancytokeratin18)ELISAKit人细胞角蛋白18X1902396TCyclin-D2(MouseCyclin-D2)ELISAKit小鼠细胞周期素D2X1902496TCgA(HumanChromogranin)ELISAKit人嗜铬蛋白AX1902596TLTC4ELISAKit大鼠白三烯C4X1902696TCyt-CELISAKit大鼠细胞色素CX1902796TpRB(Humanretinoblastomatumorsuppressorprotein)ELISAKit人视网膜母细胞瘤YZ蛋白X1902896TE(Mouseestrogen)ELISAKit小鼠雌激素X1902996T
更多资料
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大鼠血小板因子3(PF-3)ELISA KitHSP-90(Mouse Heat Shock Protein 90) ELISA Kit
- 大鼠血小板因子3(PF-3)ELISAKitHSP-90(MouseHeatShockProtein90)ELISAKit小鼠热休克蛋白90X1900396TMDAELISAKit大鼠丙二醛X1900496TSmad7(HumanMothersagainstdecapentaplegichomolog7)ELISAKit人Smad7X1900596TSODELISAKit大鼠超氧化物歧化酶X1900696TTAA(Humantumor-associatedantigen)ELISAKit人肿瘤相关抗原X1900796THSP-70(MouseHeatShockProtein70)ELISAKit小鼠热休克蛋白70X1900896TADP(Mouseadiponectin)ELISAKit小鼠脂联素X1900996THSPgp96ELISAKit大鼠血小板因子3(PF-3)ELISAKit大鼠热休克蛋白糖蛋白96X1901096TTIEG1(HumanTGF-beta-inducibleearlyresponsegene-1)ELISAKit人TGF-β诱导早期基因1X1901196T公司将ELISA试剂盒操纵中各个环节常泛起的问题我们总结一下:首先选择试剂:选择质量优良的检测试剂,严格按照试剂仿单进行操纵,操纵前将试剂在室温下平衡30-60分钟,试验以敏捷度较高、特异性较好的特点在科研上得到了广泛的应用,但操纵中的各个环节对试验的检测效果影响较大,如不留意,有可能导致显色不全、花板等结果,请避免反复冻融。特别要留意加样问题。加样:室温温育的试剂,特别是温育时间比较短的实验操纵的试剂,加样对数据的影响比较大。显色液(有A液和B液的),用前15分钟配制;尺度品:有的试剂盒中尺度品是已经配制好的,4度保留;有的是要现配制的;按照仿单操纵;浓缩生物素结合的二抗和HRP:假如需要配制,试剂盒没注明配好可以保留多久的话,尽量现配现用(用前15分钟配制);原则是ELISA试剂盒上没有指出配制物的蕴藏方式的话,应该现配现用;不要怕麻烦;还有小瓶的管子开盖前要离心,以免盖子上粘连以後总量不够。不好的操作原因:不会准确使用加样器;血清或血浆标天职离不好即进行加样;手工操纵中,加样板过多造成加样後放入孵箱前等待时间过长(特别是室内温度较高时);加完标本再加酶试剂时酶溅出孔外;参考预备实验的准确步骤,实验的事前预备工作,标本收集与保留:避免使用肉眼可见的溶血标本、高脂标本和混浊标本;2-8℃下,标本可放置24-48小时,长期保留需放置-20℃下。详细请参照:ELISA实验标本的采集与处理:ELISA实验标本的保留:试剂使用中的预备工作及留意事项:试剂盒中会有提示,一般需要预备的有:洗涤液;用前配制;有的试剂盒会标明,配好的4度下一般可以放一个月;假如没有标明,尽量用新鲜的,不要多配制。TAF(Humantumorangiogenesisfactors)ELISAKit人肿瘤血管生长因子X1901296THsp-40(MouseHeatShockProtein40)ELISAKit小鼠热休克蛋白40X1901396TCH50ELISAKit大鼠血清总补体X1901496TB7-2/sCD86ELISAKit大鼠可溶性CD86X1901596TCK-20(Humancytokeratin20)ELISAKit人细胞角蛋白20X1901696TABeta1-40(Mouseamyloidbetapeptide1-40)ELISAKit小鼠β淀粉样蛋白X1901796TCK-19(Humancytokeratin19)大鼠血小板因子3(PF-3)ELISAKitELISAKit人细胞角蛋白19X1901896TIL-8/CXCL8(Mouseinterleukin8)ELISAKit小鼠白介素8X1901996THIF-1AlphaELISAKit大鼠低氧诱导因子1αX1902096TAmylinELISAKit大鼠胰淀素X1902196TCyclin-D3(MouseCyclin-D3)ELISAKit小鼠细胞周期素D3X1902296TCK-18(Humancytokeratin18)ELISAKit人细胞角蛋白18X1902396TCyclin-D2(MouseCyclin-D2)ELISAKit小鼠细胞周期素D2X1902496TCgA(HumanChromogranin)ELISAKit人嗜铬蛋白AX1902596TLTC4ELISAKit大鼠白三烯C4X1902696TCyt-CELISAKit大鼠细胞色素CX1902796TpRB(Humanretinoblastomatumorsuppressorprotein)ELISAKit人视网膜母细胞瘤YZ蛋白X1902896TE(Mouseestrogen)ELISAKit小鼠雌激素X1902996T[详细]
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2018-09-27 10:00
产品样册
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小鼠血小板因子3(PF-3)ELISA Kit
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2015-09-29 00:00
期刊论文
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大鼠血小板因子3(PF-3)ELISA试剂盒
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2013-12-11 00:00
应用文章
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小鼠血小板因子3(PF-3)ELISA试剂盒
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2024-09-20 13:31
应用文章
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小鼠血小板因子3(PF-3)检测试剂盒
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2015-07-31 00:00
课件
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Mouse CaSR ELISA kit
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2015-10-09 00:00
操作手册
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Mouse (VLDL)ELISA Kit
- Mouseverylowdensitylipoprotein(VLDL)ELISAKitFORRESEARCHUSEONLY.NotforclinicaldiagnosisuseCATALOG#:DAG859INTRODUCTION?ThiskitallowsforthedeterminationofVLDLconcentrationsinMouseserum?Detectionofspecies:Mouse?Detectionmedium:serum,cellculturesupernates.?Assayrange:6.0μg/ml-160μg/mlPRINCIPLEOFTESTThekitassayMouseVLDLlevelinthesample,usePurifiedMouseVLDLantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddVLDLtowells,CombinedVLDLantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofMouseVLDLinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.Phone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG6342COMPOSITIONOFTHEKIT1washsolution20ml×1bottle7StopSolution6ml×1bottle2HRP-Conjugatereagent6ml×1bottle8Standard(320μg/ml)0.5ml×1bottle3Microelisastripplate12well×8strips9Standarddiluent1.5ml×1bottle4Samplediluent6ml×1bottle10Instruction15ChromogenSolutionA6ml×1bottle11Closureplatemembrane26ChromogenSolutionB6ml×1bottle12Sealedbags1STORAGECONDITIONS?Theunopenedkitshallbestoredat[2-8℃].?Foropenedkitcanbestoredat[2-8℃]forupto1month.Ifnotbeusedrecently,thestandard首ldbekeptin-20℃.WASHINGMETHOD?Manuallywashingmethod:shakeawaytheremainedliquidintheenzymeplates;placesomebibulouspapersonthetest-bed,andflaptheplatesontheupsidedownstrongly.Injectatleast0.35mlafter-dilutionwashingsolutionintothewell,andmarinate1~2minutes.Repeatthisprocessaccordingtoyourrequirements.?Automaticwashingmethod:ifthereisautomaticwashingmachine,it首ldonlybeusedinthetestwhenyouarequitefamiliarwithitsfunctionandperformance.SAMPLEPREPARATION1.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.Phone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG63432.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.ASSAYPROCEDUREStep1:Diluteandaddsample:DiluteOriginaldensityStandardasfollowtable:Step2:Setblankwellsseparately(blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame).testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl(samplefinaldilutionis5-fold),addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.Step3:Incubate:Coverwiththeadhesivestripprovided,incubatefor30minat37℃.Step4:Configurateliquid:Dilutewashsolution30-fold(or20-fold)withdistilledwater.Step5:Washing:Uncovertheadhesivestrip,discardliquid,Pipettewashingbuffertoeverywell,stillfor30sthendrain,repeat5times.Step6:Addenzyme:PipetteHRP-Conjugatereagent50μltoeachwell,exceptblankwell.Step7:Incubate:Operationwith3.Step8:Washing:Operationwith5.160μg/ml5Standard150μlOriginaldensityStandard+150μlStandarddiluent80μg/ml4Standard150μl5Standard+150μlStandarddiluent40μg/ml3Standard150μl4Standard+150μlStandarddiluent20μg/ml2Standard150μl3Standard+150μlStandarddiluent10μg/ml1Standard150μl2Standard+150μlStandarddiluentPhone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG6344Step9:Color:PipetteChromogenSolutionA50ulandChromogenSolutionBtoeachwell,avoidthelightpreservationfor15minat37℃Step10:Stopthereaction:PipetteStopSolution50μltoeachwell,Stopthereaction(thebluechangetoyellow).Step11:Calculate:takeblankwellaszero,Readabsorbanceat450nmafterPipetteingStopSolutionwithin15min.CALCULATIONOFRESULTTakethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.EXPIRATIONSixmonths[seelabelontheouterboxforthespecificdate].Phone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG634TTENTION?Thekittakesoutfromtherefrigeration首ldbebalanced15-30minutesintheroomtemperature,ifthecoatedELISAplateshavenotbeenusedupafteropening,theplate首ldbestoredinsealedbag.?washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.?addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.?ifthetestingmaterialcontentisexcessivelyhigher(ThesampleODisbiggerthanthefirststandardwell),pleasediluteSample(n-fold),Pleasediluenteandmultipliedbythedilutionfactor.(×n×5).?Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.?Thesubstrate首ldevadethelighttobepreserved.?Pleaserefertotheuserinstructionstrictly,thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.?Thepreparationofsamplesandallthereagents首ldrefertoinfectivematerialprocess.?Donotmixreagentswiththosefromotherlots[详细]
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2018-10-31 10:00
产品样册
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Protein A ELISA Kit 9333-1说明书
- P/N9333-1RepligenA蛋白ELISA试剂盒ProteinAELISAKitP/N9333-1上海起发实验试剂有限公司ProteinAELISAKitP/N9333-1专业代理,具体产品信息欢迎电询:4006551678。ProteinAELISAKitHighlightsAlargepercentageoftheworld'scommercialsupplyofantibodyreliesonaRepligenmanufacturedproteinAligandforpurificationandRepligen’sELISAkitforproductrelease.2kitsforthemostaccuratedetectionandquantitationofleachedproteinARecombinantProteinAKitMabSelectSuReKitSuperiorinterandintrakitconsistencyforunparalleledreproducibilityPolyclonalchickencaptureantibodyforgreaterProteinAspecificityBiotinylatedrabbitanti-ProteinAdetectionantibodyforgreatersensitivityMatchedstandardsforthegreatestquantitationaccuracyThreesampleprepprotocolsforsuperiormethodoptimization上海起福生物科技有限公司联系人:杨建辉400电话:4006551678办公电话:021-50724187传真:021-50724961*8006手机:15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址:上海市浦东新区绣川路561号1102室[详细]
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2018-09-29 10:02
产品样册
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大鼠血小板因子-4(PF-4)ELISA试剂盒
- 大鼠血小板因子-4(PF-4)ELISA试剂盒(用于血清、血浆、细胞培养上清液和其它生物体液内)原理本实验采用双抗体夹心ABC-ELISA法。用抗大鼠PlateletFactor4单抗包被于酶标板上,标准品和样品中的PF-4与单抗结合,加入生物素化的抗大鼠PF-4,形成免疫复合物连接在板上,辣根过氧化物酶标记的Streptavidin与生物素结合,加入底物工作液显蓝色,Z后加终止液硫酸,在450nm处测OD值,PF-4浓度与OD值成正比,可通过绘制标准曲线求出标本中PF-4浓度。试剂盒组成(2-8℃保存)酶标板(CoatedWells)96孔酶标抗体工作液(EnzymeConjugate)12ml10×标本稀释液(SampleBuffer)12ml20×浓缩洗涤液(WashBuffer)50ml标准品(Standards):200IU/瓶2瓶底物工作液(TMBSolution)12ml**抗体工作液(BiotinylatedAntibody)12ml终止液(StopSolution)12ml准备试剂与收集血样1.收集标本:血清、血浆(EDTA、柠檬酸盐、肝素抗凝)、细胞培养上清液、组织匀浆等尽早检测,2-8℃保存48小时;更长时间须冷冻(-20℃或-70℃)保存,避免反复冻融。血清、血浆作1:10稀释(取20ul,加标本稀释液180ul,稀释10倍)。细胞培养上清可以不做稀释直接检测。2.标准品液配制:使用前加入1ml蒸馏水混匀,配成200IU/ml的溶液。设标准管8管,**管加标本稀释液900ul,第二至第八管加入标本稀释液500ul。在**管中加入200IU/ml的标准品溶液100ul混匀后用加样器吸出500ul,移至第二管。如此反复作对倍稀释,从第七管中吸出500ul弃去。第八管为空白对照。3.10×标本稀释液用蒸馏水作1:10倍稀释(示例:1ml浓稀释液+9ml蒸馏水)。4.洗涤液:用重蒸水1:20稀释(示例:1ml浓缩洗涤液加入19ml的重蒸水)检测程序1.加样:每孔各加入标准品或待测样品100ul,将反应板充分混匀后置37℃120分钟。2.洗板:用洗涤液将反应板充分洗涤4-6次,向滤纸上印干。3.每孔中加入**抗体工作液100ul。将反应板充分混匀后置37℃60分钟。4.洗板:同前。5.每孔加酶标抗体工作液100ul。将反应板置37℃30分钟。6.洗板:同前。7.每孔加入底物工作液100ul,置37℃暗处反应15分钟。8.每孔加入100ul终止液混匀。9.30分钟内用酶标仪在450nm处测吸光值。结果计算与判断1.所有OD值都应减除空白值后再行计算。2.以标准品20、10、5、2.5、1.25、0.625、0.312、0IU/ml为横坐标,OD值为纵坐标,在坐标纸上作图,画出标准曲线。3.根据样品OD值在该曲线图上查出相应PF-4含量,再乘上稀释倍数即可。试剂盒性能1.灵敏度:Z小的PF-4检测浓度小于0.2IU/ml。2.特异性:可同时检测重组或天然的大鼠PF-4。不与大鼠其它细胞因子有交叉反应。3.重复性:板内、板见变异系数均小于12%。注意事项1.以上标准孔及待测样品均建议做复孔,每次测定应同时做标准曲线。2.洗涤过程很关键。洗涤不充分将导致极ng确度误差及OD值错误地升高。3.板条开封后剩余板条要再封好,保持板条干燥。4.本试剂盒宜置4oC冰箱保存。5.本试剂盒仅用于科研,不能用于临床诊断![详细]
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2018-09-13 10:00
产品样册
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大鼠血小板激活因子(PAF)ELISA试剂盒
- 大鼠血小板激活因子(PAF)ELISA试剂盒[详细]
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2024-09-15 16:45
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大鼠血小板因子4(PF-4/CXCL4)ELISA试剂盒
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Protein A ELISA Kit P/N 9000-1说明书
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产品样册
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大鼠神经营养因子3(NT-3)ELISA试剂盒
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期刊论文
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大鼠磷酸化核因子κBYZ蛋白α(pIKBα)ELISA Kit
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2015-10-08 00:00
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Ultra Sensitive Mouse Insulin ELISA Kit,crystaLchem,90080
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产品样册
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大鼠热休克蛋白90(HSP-90)ELISA试剂盒
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大鼠基质金属蛋白酶YZ因子3(TIMP-3)ELISA试剂盒
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选购指南
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大鼠β-血小板球蛋白(βTG)ELISA试剂盒
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2016-06-23 00:00
课件
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大鼠血小板活化因子(PAF)ELISA试剂盒
- 大鼠血小板活化因子(PAF)ELISA试剂盒(用于血清、血浆、细胞培养上清液和生物体液内)原理本实验采用双抗体夹心ABC-ELISA法。用抗大鼠PAF单抗包被于酶标板上,标准品和样品中的PAF与单抗结合,加入生物素化的抗大鼠PAF,形成免疫复合物连接在板上,辣根过氧化物酶标记的Streptavidin与生物素结合,加入底物工作液显蓝色,Z后加终止液硫酸,在450nm处测OD值,PAF浓度与OD值成正比,可通过绘制标准曲线求出标本中PAF浓度。试剂盒组成(2-8℃保存)酶标板(CoatedWells)96孔酶标抗体工作液(EnzymeConjugate)12ml10×标本稀释液(SampleBuffer)12ml20×浓缩洗涤液(WashBuffer)50ml标准品(Standards):1000mU/瓶2瓶底物工作液(TMBSolution)12ml**抗体工作液(BiotinylatedAntibody)12ml终止液(StopSolution)12ml准备试剂与收集血样1.收集标本:血清、血浆(EDTA、柠檬酸盐、肝素抗凝)、细胞培养上清液、组织匀浆等尽早检测,2-8℃保存48小时;更长时间须冷冻(-20℃或-70℃)保存,避免反复冻融。血清测定前用标本稀释液至少作1:10稀释(取20ul,加标本稀释液180ul,稀释10倍)。2.标准品液配制:使用前加入1ml蒸馏水混匀,配成1000mU/ml的溶液。设标准管8管,**管加标本稀释液900ul,第二至第八管加入标本稀释液500ul。在**管中加入1000mU/ml的标准品溶液100ul混匀后用加样器吸出500ul,移至第二管。如此反复作对倍稀释,从第七管中吸出500ul弃去。第八管为空白对照。3.10×标本稀释液用蒸馏水作1:10倍稀释(示例:1ml浓稀释液+9ml蒸馏水)。4.洗涤液:用重蒸水1:20稀释(示例:1ml浓缩洗涤液加入19ml的重蒸水)检测程序1.加样:每孔各加入标准品或待测样品100ul,将反应板充分混匀后置37℃120分钟。2.洗板:用洗涤液将反应板充分洗涤4-6次,向滤纸上印干。3.每孔中加入**抗体工作液100ul。将反应板充分混匀后置37℃60分钟。4.洗板:同前。5.每孔加酶标抗体工作液100ul。将反应板置37℃30分钟。6.洗板:同前。7.每孔加入底物工作液100ul,置37℃暗处反应15分钟。8.每孔加入100ul终止液混匀。9.30分钟内用酶标仪在450nm处测吸光值。结果计算与判断1.所有OD值都应减除空白值后再行计算。2.以标准品100、50、25、12.5、6.25、3.1、1.56、0mU/ml为横坐标,OD值为纵坐标,在坐标纸上作图,画出标准曲线。3.根据样品OD值在该曲线图上查出相应PAF含量,再乘上稀释倍数即可。试剂盒性能1.灵敏度:Z小的PAF检测浓度小于1mU/ml。2.特异性:可同时检测重组或天然的大鼠PAF。不与大鼠其它细胞因子有交叉反应。3.重复性:板内、板见变异系数均小于10.6%。注意事项1.以上标准孔及待测样品均建议做复孔,每次测定应同时做标准曲线。2.洗涤过程很关键。洗涤不充分将导致极ng确度误差及OD值错误地升高。3.板条开封后剩余板条要再封好,保持板条干燥。4.本试剂盒宜置4oC冰箱保存。5.本试剂盒仅用于科研,不能用于临床诊断![详细]
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2018-09-13 10:00
产品样册
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大鼠血小板活化因子(PAF)ELISA试剂盒
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2024-10-07 08:28
报价单
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