Recombinant Biotinylated Human GITR/TNFRSF18 Protein,His-Avi Tag

分子别名（Synonyms）

GITR-D; GITR;CD357; AITR

表达区间及表达系统（Source）

Biotinylated Human GITR/TNFRSF18 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln26-Glu161.[Accession | Q9Y5U5-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 17.2 kDa. Due to glycosylation, the protein migrates to 26-28 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Human GITR Ligand Trimer, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GITR, His Tag with the EC50 of 77.9ng/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.