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性能参数
|
分子别名(Synonyms) |
ANG-5; ANGPT5;ANGPTL3; FHBL2 |
|
表达区间及表达系统(Source) |
Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser17-Lys219.[Accession | Q9Y5C1] |
|
分子量大小(Molecular Weight) |
The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on SDS-PAGE result. |
|
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
|
纯度(Purity) |
> 95% as determined by SDS-PAGE |
|
活性(Activity) |
ELISA Data: Immobilized Human ANGPTL3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. |
|
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
重构方法(Reconstitution) |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
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