Recombinant Porcine IL-2 Protein

Synonyms

T-cell Growth Factor, TCGF

Accession

P26891

GeneID

396868

Source

E.coli-derived Porcine IL-2, Ala21-Thr154.

Molecular Weight

Approximately 15.2 kDa.

AA Sequence

APTSSSTKNT KKQLEPLLLD LQLLLKEVKN YENADLSRML TFKFYMPKQA TELKHLQCLV EELKALEGVL NLGQSKNSDS ANIKESMNNI NVTVLELKGS ETSFKCEYDD ETVTAVEFLN KWITFCQSIY STLT

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 96% by SDS-PAGE and HPLC analyses.

Biological Activity

 The ED₅₀ as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.