Human IFN-α2c

Source

Yeast-derived Human IFN-α2c protein,Cys24-Glu188(K23R,H24R.

Molecular Weight

Approximately  19.3 kDa.

AA Sequence

CDLPQTHSLG SRRTLMLLAQ MRRISLFSCL KDRRDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The activity is determined by the cytopathic effect inhibition assay.

Endotoxin

< 0.1 EU/μg of protein as determined by LAL method.

Formulation

Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4, with 0.02 % Tween-20.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.