Recombinant Human IFN-alpha-1B Protein重组人干扰素α1b

Synonyms

Human IFN-α1b

Accession

P01562

GeneID

3439

Source

E.coli-derived Human IFN-α1b protein,Cys24-Glu189(A115).with an N-terminal Met.

Molecular Weight

Approximately 19.5 kDa

AA Sequence

MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDE DLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNVDSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 × 10⁸ IU/mg.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4, containing 4% mannitol and 1% HSA.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.