Recombinant Human Galectin-3 Protein

Synonyms

AGE-R3, CBP35, GAL3, L29, LGALS3, Mac-2

Uniprot No.

P17931

Source

Yeast-derived Human Galectin-3, Ala2-Ile250.

Molecular Weight

Approximately 26.0 kDa.

Purity

> 90% by SDS-PAGE.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

 Biological Activity

Recombinant human Galectin-3 is able to agglutinate red blood cells at a minimum effective concentration of 25µg/ml.

Formulation

Lyophilized from a 0.2 µm filtered solution in 1 × PBS, pH 7.4, 1%BSA.

Reconstitution

1.We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL.

2.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.

Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options.