CD276人重组蛋白

蛋白名称:CD276人重组蛋白，11188-H08H

蛋白构建:A DNA sequence encoding the human CD276 (Q5ZPR3-1) extracellular domain (Met1-Thr461) was expressed with a polyhistidine tag at the C-terminus.

表达宿主:HEK293 Cells

蛋白纯度:≥ 98 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).

蛋白活性:

1.Immobilized Anti-Human B7H3 antibody, human IgG1  at 2 μg/mL (100 μL/well) can bind Recombinant Human B7-H3 / CD276 Protein (ECD,His Tag) (Cat:11188-H08H), The EC50 is 2.5-7.5 ng/mL.

2.Loaded Enoblituzumab on proA Biosensor, can bind Recombinant Human B7-H3 Protein, His Tag (Cat. No. 11188-H08H) with an affinity constant of 1.06 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method

预测N端:Leu 29

蛋白分子量:The recombinant human CD276 consists of 444 amino acids and predictes a molecular mass of 48 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human CD276 is approximately 68 kDa due to glycosylation. The molecular weight of this protein is around 61 kDa verified by SEC-MALS (Routinely tested).

蛋白NP号:Q5ZPR3-1

蛋白氨基酸序列:Met1-Thr461

蛋白标签:C-His

欢迎去义翘神州网查看CD276人重组蛋白，11188-H08H详细信息。网址：https://cn.sinobiological.com/recombinant-proteins/human-b7-h3-11188-h08h

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