CD19人重组蛋白

蛋白名称:CD19人重组蛋白，11880-H08W1-SA

蛋白构建:A DNA sequence encoding the extracellular domain (Met1-Lys291) of human CD19 (P15391-1) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with APC (Excitation Max.= 651 nm, Emission Max.= 662 nm).

表达宿主:HEK293 Stable Cells

蛋白纯度:

蛋白活性:

1.Flow cytometric analysis of anti-CD19 CAR expression. 5e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:42 dilution of APC-conjugated human CD19 Protein (Site-Specific APC-Conjugated, ECD, His Tag, Cat. No. 11880-H08W1-SA) and negative control protein. Non-transduced 293 cells were used as a control (left). APC signal was used to evaluate the binding activity (QC tested).

2.Binding activity of APC-conjugated CD19 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human CD19 Protein (Site-Specific APC-Conjugated, ECD, His Tag, Cat. No. 11880-H08W1-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).

3.Flow cytometric analysis using APC conjugated CD19 protein. 5e5 of anti-CD19 CAR-293 cells were stained with APC-conjugated human CD19 protein (Site-Specific APC Conjugation, ECD, His Tag, Cat. No. 11880-H08W1-SA). Non-transduced 293 cells were used as a control (Black Histogram). The protein exhibited stable performance after multiple freeze-thaw cycles (10 cycles)

4.Flow cytometric analysis using APC conjugated CD19 protein. 5e5 of anti-CD19 CAR-293 cells were stained with APC-conjugated human CD19 protein from two vendors (competitor A and SINO). Non-transduced 293 cells were used as a control (Black Histogram). The results demonstrated that SINO’s 11880-H08W1-SA has a  higher fluorescence intensity than that of the competitor.

蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.

预测N端:Pro 20

蛋白分子量:The protein has a predicted molecular mass of 31.6 kDa.

蛋白NP号:P15391-1

蛋白氨基酸序列:Met1-Lys291

蛋白标签:C-His

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