ACE2人重组蛋白

蛋白名称:ACE2人重组蛋白，10108-H02H

蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Ser 740) of human ACE2 precursor (NP_068576.1) was expressed with the fused Fc region of human IgG1 at the C-terminus.

表达宿主:HEK293 Cells

蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

蛋白活性:

1.Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 (2019-nCoV) Spike S1-His (Cat: 40591-V08B1) at 2 μg/mL (100 μL/well) can bind human ACE2 (hFc tag) (Cat: 10108-H02H), the * of ACE2 is 5-35 ng/mL.

2.Captured ACE2（10108-H02H on proA Chip can bind SARS-CoV-2 B.1.1.529 (Omicron) Spike S1 Protein (Cat.No.40591-V08H41) with an affinity constant of 11.78 nM as determined in a SPR assay (Biacore T200)(Routinely tested).

3.Captured ACE2（10108-H02H on anti-Human IgG Fc via CM5 Chip can bind SARS-CoV-2 B.1.1.529 (Omicron) Spike RBD Protein (Cat. No. 40592-V08H121) with an affinity constant of 5.667 nM as determined in a SPR assay (Biacore T200)(Routinely tested).

4.Loaded SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (Cat. No. 40592-V05H) on AMC Biosensor, can bind Recombinant Human ACE2 Protein , hFc Tag (Cat. No. 10108-H02H) with an affinity constant of 7.34 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method

预测N端:Gln 18

蛋白分子量:The recombinant human ACE2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 961 amino acids and predicts a molecular mass of 110.3 kDa. As a result of glycosylation, the rhACE2/Fc monomer migrates as approximately 145-150 kDa band in SDS-PAGE under reducing conditions.

蛋白NP号:NP_068576.1

蛋白氨基酸序列:Met1-Ser740

蛋白标签:C-human IgG1-Fc

欢迎去义翘神州网查看ACE2人重组蛋白，10108-H02H详细信息。网址：https://cn.sinobiological.com/recombinant-proteins/human-ace2-10108-h02h

义翘神州研发团队拥有多年的重组蛋白研发经验。多年来，我们的团队已开发出超过9300余种重组蛋白，涵盖免疫检查点、抗体药物靶点、CAR-T细胞治疗靶点、Fc受体、流感病毒蛋白以及细胞因子等热门研究领域。义翘神州重组蛋白具有高纯度、高生物活性和低内毒素水平等特性，可为生物药靶点发现、蛋白质结构和功能分析、细胞疗法和重组酶酶学性质等科学研究提供支持。

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