IL6人重组蛋白

蛋白名称:IL6人重组蛋白，10395-HNAE

蛋白构建:A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val30-Met212) was expressed with an initial Met at the N-terminus.

表达宿主:E. coli

蛋白纯度:≥ 95 % as determined by SDS-PAGE

蛋白活性:

1. Immobilized Human IL-6 (Cat:10395-HNAE) at 2 μg/mL (100 μL/well) can bind Human IL-6R hFc(Cat:10398-H02H), the * of Human IL-6R hFc(Cat:10398-H02H) is 3.0-10.0 ng/mL.

2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The * for this effect is 0.1-0.8 ng/mL.

3. Loaded Recombinant Human IL-6R Protein, His Tag (Cat. No. 10398-H08H)  on HIS1K Biosensor, can bind Recombinant Human IL-6 Protein (Cat. No. 10395-HNAE) with an affinity constant of 75.9 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

蛋白内毒素:< 10 EU per mg of the protein.

预测N端:Met

蛋白分子量:The recombinant human IL6 consists of 184 amino acids and predicts a molecular mass of 20.95 kDa. It migrates as an approximately 19.7 kDa band in SDS-PAGE under reducing conditions.

蛋白NP号:NP_000591.1

蛋白氨基酸序列:Val30-Met212

蛋白标签:Native

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