CD47小鼠重组蛋白

蛋白名称:CD47小鼠重组蛋白，57231-M49H-B

蛋白构建:A DNA sequence encoding the Mouse CD47 (Q61735-1)(Met1-Lys140) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

表达宿主:HEK293 Cells

蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

蛋白活性:Immobilized Recombinant Mouse CD47 Protein (His & AVI Tag), Biotinylated (Cat: 57231-M49H-B）at 2 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL，100 μL/well) can bind Recombinant Mouse SIRP alpha / CD172a Protein (Fc Tag) (Cat: 50956-M02H）, the * is 14-52 ng/mL.

蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.

预测N端:Gln 19

蛋白分子量:The recombinant Mouse CD47 consists of 148 amino acids and predicts a molecular mass of 17.1 kDa. It migrates as an approximately 38.8 kDa band in SDS-PAGE under reducing conditions.

蛋白NP号:Q61735-1

蛋白氨基酸序列:Met1-Lys140

蛋白标签:C-His-AVI

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