IL4人重组蛋白

蛋白名称:IL4人重组蛋白，11846-HNAE

蛋白构建:A DNA sequence encoding the mature form of human IL4 isoform 1 (P05112-1) (His 25-Ser 153) was expressed and purified with an initial Met.

表达宿主:E. coli

蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

蛋白活性:

1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The * for this effect is 0.05-0.25 ng/mL.

2.Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with IL4 (Cat# 11846-HNAE) (100 ng/mL, 15min; +), using Phospho-Stat6 (Tyr641) Antibody, Rabbit MAb (Cat# 111168-R0044) at 1:10000 dilution (upper) or Anti-STAT6 Antibody, Rabbit PAb (Cat# 201061-T36) at 1:1000 dilution (lower)(Routinely tested).

3.Loaded Recombinant Human IL-4Rα & IL-13Rα1 Heterodimer Protein, hFc Tag (Cat. No. CT117-H02H) on proA Biosensor, can bind Recombinant Human IL-4 Protein (Cat. No. 11846-HNAE) with an affinity constant of 0.571 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

蛋白内毒素:< 5 EU per mg of the protein.

预测N端:Met

蛋白分子量:The recombinant human IL4 consisting of 130 amino acids and migrates with an apparent molecular mass of 15.1 kDa as predicted.

蛋白NP号:P05112-1

蛋白氨基酸序列:His25-Ser153

蛋白标签:Native

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