载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏ZX

NTCC典型培养物保藏中心-BioVector质粒载体菌种细胞基因保藏中心，供应数十万种质粒、载体、菌种、基因、细胞、感受态细胞等产品。NTCC典型培养物保藏中心-BioVector质粒载体菌种细胞基因保藏中心资源种类齐全，涵盖标准菌种、质控菌株、食品检测菌种、药品检测菌种、环境监测菌种、NTCC/DSMZ/ATCC菌种细胞、克隆载体、真核表达载体、原核表达载体、动物细胞表达载体、慢病毒载体、腺病毒逆病毒载体、RNAi基因沉默基因敲除载体、抗体表达载体GS加压筛选悬浮细胞表达系统、信号通路报告载体、亚细胞定位载体、荧光蛋白载体、昆虫细胞/杆状病毒表达载体、植物表达载体RNAi干扰沉默载体、农杆菌菌株与感受态细胞、启动子载体、诱导调控载体、毕赤酵母/酿酒酵母表达载体、酵母表面展示系统、酵母单杂双杂三杂交系统、噬菌体展示抗体系统、枯草芽孢杆菌表达载体系统、乳酸菌表达系统、分支杆菌表达系统、假单胞菌表达载体基因敲除质粒、细胞自噬质粒载体LC3、细胞永生化质粒载体SV40/hTERT、iPS干细胞重编程载体系统、转座子载体、CRISPR/Cas9载体系统TALEN基因编辑系统、慢病毒荧光表达载体、果蝇/线虫表达载体、革兰氏阳性菌/金葡菌/链霉菌链球菌表达载体敲除载体、稀有基因合成克隆等几十种类型数十万个品种。并可提供质粒/菌种保藏鉴定、基因合成、基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等实验技术服务。BioVector NTCC Inc.还可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。
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 T98  CW504 derived transconjugant RifR NalR ErmRwith plasmid pCpb2 from CP1Δcpb2::ErmRAM This study          
 NE01 1 Necrotic enteritis [16]          
 NE04 10 Necrotic enteritis [16]          
 NE06 2 Necrotic enteritis [16]          
 NE09 4 Necrotic enteritis [16]          
 NE10 3 Necrotic enteritis [16]          
 NE14 5 Necrotic enteritis [16]          
 NE15 6 Necrotic enteritis [16]          
 NE19 8 Necrotic enteritis [16]          
 NE20 9 Necrotic enteritis [16]          
 NE23 10 Necrotic enteritis [16]          
 NE28 13 Necrotic enteritis [16]          
 NE30 14 Necrotic enteritis [16]          
 NE32 15 Necrotic enteritis [16]          
 NE42 16 Necrotic enteritis [16]          
 NE57 22 Necrotic enteritis [16]          
 H+18 8 Healthy [16]          
 H+22 1 Healthy [16]          
 H+26 11 Healthy [16]          
 H+27 12 Healthy [16]          
 H+34 10 Healthy [16]          
 H+60 6 Healthy [16]          
 H-16 7 Healthy [16]          
 H-45 17 Healthy [16]          
 H-46 19 Healthy [16]          
 H-47 18 Healthy [16]          
 H-54 20 Healthy [16]          
Plasmids   [16]          
 pMTL007  Clostridial vector for expression of ClosTron, containingErmRAM, ColE1, CmR [19]          
 pMTL-netB  pMTL007 containing intron retargeted to C. perfringensnetB(sense insertion at 461–462 bp) This study          
 pMTL-cpb2  pMTL007 containing intron retargeted to C. perfringenscpb2(sense insertion at 390–391 bp) This study          
pAES1 P. aestuarii DSM 19484 2925 64.4 62 [43] 2 REP, MOB       
pAES2  4502 57.9  5 REP, R-M       
pAES3  5434 51.7  7 REP, TA       
pAES4  5850 58.6  9 REP, MOB, TA       
pAES7  13,005 60.1  15 REP, MOB, TA(2)       
pHAE1 P. haeundaensis LG P-21903 5301 58.8 66.9 [44] 4 REP       
pHAE2  5777 53.6  5 REP, TA       
pMARC1 P. marcusii DSM 11574 5122 49 66 [45] 6 REP, TA       
pMARC2  5789 51.6  6 REP, MOB       
pMARC3  10,672 59  10 REP, PAR, MOB, TA       
pMARC4  15,289 53.4  15 REP, MOB, TA       
pMOS2 P. marcusii OS22 6410 54 66 [45] 5 REP, MOB       
pMOS6  7672 63.1  12 REP, MOB, TA       
pMOS7  5979 50  7 REP, TA       
E. coli WH207 tet50 galK rpsL thi lacX74 recA13 Tn10 tetA-lacZ transcriptional             
fusion bet+ gam+ cIII+ cI+             
Smith and Bertrand, 1988;             
Wissmann et al., 1991             
B. subtilis WH557 trpC2 lacA::Pt17-tetR lox66-aphAIII-lox71 Bertram et al., 2005             
B. subtilis WH558 trpC2 lacA::Pt17-tetR lox66-aphAIII-lox71 amyE::Pxyl/tet-lacZ Bertram et al., 2005             
B. subtilis WH560 trpC2 amyE::Pxyl/tet-lacZ Bertram et al., 2005             
B. subtilis RAB100 trpC2 lacA::Pt17-tetR-lox72 amyE::Pxyl/tet-lacZ this study             
B. subtilis RAB101 trpC2 lacA::Pt17-tetR-lox66-aphAIII-lox71-tetR;lox72 amyE::Pxyl/tet-lacZ this study             
B. subtilis RAB102 trpC2 lacA::Pt17-tetRlox72/1 lox72 amyE::Pxyl/tet-lacZ this study             
Plasmids             
pCrePA EmR, PpagA-cre thermosensitive, expression of cre in Bacillus Pomerantsev et al., 2006             
pWH1925 ApR, pUC19 derivative, no tetR Bertram et al., 2004             
pWH1926 ApR, pUC19 derivative, constitutive expression of tetR Kamionka et al., 2006             
pWH1926-F pWH1926 derivative, tetRFRT this study             
pWH1926-F-inv pWH1926 derivative, tetRFRT-inv this study             
pWH1926-L1 pWH1926 derivative, tetRlox72/1 this study             
pWH1926-L1-inv pWH1926 derivative, tetRlox72/1-inv this study             
pWH1926-L2 pWH1926 derivative, tetRlox72/2 this study             
pWH1926-L2-inv pWH1926 derivative, tetRlox72/2-inv this study             
pWH1926-TLKLT pWH1926 derivative, tetR-lox66-aphAIII-lox71-tetR this study             
pWH1411BD CmR, pACYC177 derivative, tetR Scholz et al., 2003             
pWH1411-TLKLT pWH1411BD derivative, tetR-lox66-aphAIII-lox71-tetR this study             
pWH1411-TLKLTextd pWH1411BD derivative, tetR-lox66-aphAIII-lox71-tetR ‘lacA’ This study             
Haemophilus parasuis             
SC096 Serovar 4 clinical isolate, transformable Laboratory collection           
SC003 Serovar 4 clinical isolate, non-transformable Laboratory collection           
SC045 Serovar 4 clinical isolate, non-transformable Laboratory collection           
SC062 Serovar 5 clinical isolate, non-transformable Laboratory collection           
SC089 Serovar 5 clinical isolate, non-transformable Laboratory collection           
SC086 Serovar 5 clinical isolate, non-transformable Laboratory collection           
SC028 Serovar 13 clinical isolate, non-transformable Laboratory collection           
SW114 Serovar 3 reference, non-transformable Kielstein & Rapp-Gabrielson (1992)           
Nagasaki Serovar 5 reference, non-transformable Kielstein & Rapp-Gabrielson (1992)           
C5 Serovar 8 reference, non-transformable Kielstein & Rapp-Gabrielson (1992)           
84-17975 Serovar 13 reference, non-transformable Kielstein & Rapp-Gabrielson (1992)           
SC096-1 SC096 hepII::Gm This work           
SC096-2 SC096 ΔompP2::Gm This work           
SC096-3 SC096 ΔompP2::Gm hepII::ompP2+kan This work           
Plasmids             
pMD-19T AmpR, T-vector Takara           
p34s-Gm GmR, Gm resistance cassette-carrying vector Yamanaka et al. (1995)           
pBAD18-Km KanR, Km resistance cassette-carrying vector Guzman et al. (1995)           
pK18mobsacB KanR, suicide and narrow-broad-host vector Schafer et al. (1994)           
pZB1 KanR, a 990-bp fragment containing the upstream and downstream sequences of the OmpP2 gene in pK18mobsacB This work           
pZB2 KanR GmR, a 1.790-kp fragment containing the motif of 5′-ACCGAACTC and the ΔompP2::Gm cassette in pK18mobsacB This work           
pZB3 KanR GmR, a 2.803-kp fragment containing hepII::GmR cassette in pK18mobsacB This work           
pZB4 KanR GmR, a 1.790-kp fragment containing the motif of 5′-ACCGCTTGT and the ΔompP2::Gm cassette in pK18mobsacB This work           
pZB5 KanR GmR, a 4.313-kp fragment containing containing hepII::ompP2+kan cassette in pK18mobsacB This work           
Serratia plymuthica             
G3 WT Rif r , isolated from stems of wheat in Taian, China 13           
hfq- (hfq::Gm) Rif r, Gmr, an hfq insertion mutant of strain G3 This study           
hfq-/pUCP26-hfq Rif r, Gmr, Tc r, the complementary strain of the hfq mutant This study           
hfq-/pUCP26 Rif r, Gmr, Tc r, the hfq mutant containing pUCP26 as control This study           
Escherichia coli             
DH5alpha F− recA1 endA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1D(lacZYA盿rgF) U169 k− (u80dlacZDM15) 19           
S17-1 thi pro hsdR recA; chromosomal RP4; Tra+; Sm/Spr 20           
Plasmids             
pMD19-T Apr, cloning and sequencing vector Takara           
p19T-hfq pMD19-T carrying the full hfq gene, Apr This study           
p34S-Gm Source of Gmr cassette 21           
pDM-4 Cmr, oriT+ sacB suicide vector 22           
pDM4-hfq::Gm pDM4 carrying hfq::Gm, Gmr ,Cmr This study           
pEXT20 Apr, the tac promoter 23           
pUCP26 Tcr, broad host range vector 24           
p26-hfq pUCP26 carrying the hfq gene under the control of Ptac This study           
pBluelux Apr, a promoter-less luxCDABE cassette in pBluescript II 25           
pPprn::lux pUCP26 with a prnA promoter fusion to luxCDABE, Tcr This study           
pME6015 pVS1-p15A shuttle vector for translational lacZ fusions, Tcr 26           
pME6015-prnA pME6015 with a prnA′-′lacZ translational fusion, Tcr This study           
pME6015-rpoS pME6015 with a rpoS′-′lacZ translational fusion, Tcr This study           
pME6015-hfq pME6015 with a hfq′-′lacZ translational fusion, Tcr This study           
    S. thermophilus             
        ATCC 19258 Glu+ Lac+ Man− ATCC           
        SMQ-119 Glu+ Lac+ Man− 33           
        SMQ-173 Glu− Lac+ Man− 33           
        SMQ-301 Glu− Lac+ Man− 47           
    S. salivarius             
        ATCC 25975 Glu+ Lac+ Man+ ATCC           
    E. coli             
        DH5α supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 18           
        XL1 Blue supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac− F′[proAB+ lacIq lacZΔM15 Tn10 (Tetr)] Stratagene           
        BL21(DE3) F− ompT hsdSB(rB−mB−) gal dcm (DE3) Novagen           
        LMG194 F− ΔlacX74 galE thi rpsL ΔphoA (PvuII) Δara714 leu::Tn10 Invitrogen           
Plasmids             
    pBAD-HisB Expression vector, Ampr Invitrogen           
    pET28a Expression vector, Kanr Novagen           
    pST118 S. thermophilus His6-HPr fusion in pBAD-HisB This work           
    pST119 S. thermophilus His6-IIABMan fusion in pET28a This work           
    pST121 S. thermophilus His6-EI fusion in pET28a This work           
Strain SalmonellaSerovar Genotype/Phenotypea Reference          
χ9558 Typhimurium ∆pmi-2426 ∆(gmd-fcl)-26 ∆Pfur81::TT araC PBADfur ∆Pcrp527::TT araC PBADcrp ∆asdA27::TT araC PBADc2 ∆araE25 ∆araBAD23 ∆relA198::araC PBADlacI TT ∆sopB1925 ∆agfBAC811, RpoS+ [39]          
χ9633 Typhi ISP1820 ∆Pcrp527::TT araC PBADcrp ∆Pfur81::TT araC PBADfur ∆pmi-2426 ∆(gmd-fcl)-26 ∆relA198::araC PBADlacI TT ∆araE25 ∆araBAD23 ∆tviABCDE10 ∆agfBAC811 ∆sopB1925 ∆asdA33, RpoS+ [13]          
χ9639 Typhi Ty2 ∆Pcrp527::TT araC PBADcrp ∆Pfur81::TT araC PBADfur ∆pmi-2426 ∆(gmd-fcl)-26 ∆relA198::araC PBADlacI TT ∆araE25 ∆tviABCDE10 ∆agfBAC811 ∆sopB1925 ∆asdA33, RpoS− [13]          
χ9640 Typhi Ty2 ∆Pcrp527::TT araC PBADcrp ∆Pfur81::TT araC PBADfur ∆pmi-2426 ∆(gmd-fcl)-26 ∆relA198::araC PBADlacI TT ∆araE25 ∆tviABCDE10 ∆agfBAC811 ∆sopB1925 ∆asdA33, RpoS+ [13]          
χ3761 Typhimurium wild type [40]          
χ3744 Typhi ISP1820 wild type [41]          
χ3769 Typhi Ty2 rpoS [42]          
χ8438 Typhi Ty2 RpoS+ [43]          
Plasmid  Descriptionb           
pWSK129  pSC101 ori, Kanr [44]          
pYA3493  pBR ori, Asd+ vector with bla SS-based periplasmic antigen secretion [45]          
pYA4088  Encodes the α-helical region of PspA (aa 3-285) in pYA3493 [46]          
             
    pGemTEasy T/A cloning Promega           
    pMV306 Mycobacterial integrating vector, Hygr Med-Immune Inc.           
    p2NIL Recombination vector, nonreplicating in mycobacteria, Kanr 17           
    pGoal17 Source of PacI cassette, Ampr 17           
    pJam2 Shuttle vector carrying inducible Pami promoter, Kanr 30           
    PMV306GM pMV306 with defective Hygr gene (digested with EcoRI, filled-in resultant cohesive ends, and self-ligated) carrying Gmr gene in KpnI site This study           
    pMVPami pMV306 with Pami promoter of pJam2 cloned into HindIII-XbaI restriction sites This study           
    pMV306tetR Mycobacterial integrating vector carrying tetracycline repressor, Kmr 7           
    pSE100 Shuttle vector carrying inducible Ptet promoter, Hygr 7           
Vectors used for gene replacement             
    pMK104 M. smegmatis 5′ ΔligA PstI-HindIII fragment including 5′ end and its upstream region (1,754 bp) in p2NIL, Kanr This study           
    pMK106 M. smegmatis ΔligA HindIII-BamHI fragment including 3′ end and its downstream region (1,741 bp) in pMK104, Kanr This study           
    pMK107 pMK106 with PacI cassette from pGoal17, Kanr This study           
    pMK140 pMK104 with Hygr gene cloned between 5′ and 3′ fragments of ligA (ΔligA::Hygr), Kanr Hygr This study           
    pMK141 pMK140 with PacI cassette from pGoal17, Kanr Hygr This study           
Overproduction vectors             
    pMK114 ligAMs under Pami promoter in pJam2, Kanr This study           
    pMK129 ligAMs under Pami promoter in pJam2, Kanr This study           
    pMKB144 ligBMs under Pami promoter in pJam2, Kanr This study           
    pMKC146 ligC1Ms under Pami promoter in pJam2, Kanr This study           
    pMKC148 ligC2Ms under Pami promoter in pJam2, Kanr This study           
    pMK113 ligDMs under Pami promoter in pJam2, Kanr This study           
    pJamligT4 bacteriophage T4 ligase under Pami promoter in pJam2, Kanr This study           
    pMK124 ligAMs under Pami promoter of pMK114 cloned in pMV306, Hygr This study           
    pMK130 ligAMt under Pami promoter of pMK129 cloned in pMV306, Hygr This study           
    pMK155 ligBMs under Pami promoter of pMKB144 cloned in pMV306GM, Gmr This study           
    pMK152 ligC1Ms under Pami promoter of pMKC146 cloned in pMV306GM, Gmr This study           
    pMK154 ligC2Ms under Pami promoter of pMKC148 cloned in pMV306GM, Gmr This study           
    pMK150 ligDMs under Pami promoter of pMK113 cloned in pMV306GM, Gmr This study           
    pMVligAEc ligAEc cloned under control of Pami promoter in pMVPami, Hygr This study           
    pMVligT4 Bacteriophage T4 ligase under Pami promoter of pJamligT4 cloned in pMV306, Hygr This study