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Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein,monomeric hFc Tag

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详细介绍

性能参数

表达区间及表达系统（Source）	Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-terminal. It contains Met112-Leu260.[Accession \| P27548]
分子量大小（Molecular Weight）	The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by Tris-Bis PAGE
活性（Activity）	ELISA Data: Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

Ver. CN20230620