Recombinant Human GITR Ligand/TNFSF18 Trimer Protein,monomeric hFc Tag

分子别名（Synonyms）

TNFSF18; GITRL; TL6;

表达区间及表达系统（Source）

Human GITR Ligand/TNFSF18 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus. It contains Gln50-Ser177.[Accession | Q9UNG2]

分子量大小（Molecular Weight）

The protein has a predicted MW of 70.2 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

SPR Data: Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay .

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.