Recombinant Human&Mouse Chimeric WT-1 (HLA-A*02:01) Complex Protein,His-Avi Tag

表达区间及表达系统（Source）

Recombinant Human&Mouse Chimeric WT-1 (HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide.[Accession | A0A140T913(Human HLA-A*02:01 α1&α2)

&P01897(Mouse HLA-A*02:01 α3)&P61769(B2M)&RMFPNAPYL]

分子量大小（Molecular Weight）

The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per ug by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE

> 95% as determined by HPLC

活性（Activity）

ELISA Data: Immobilized Human&Mouse Chimeric WT-1 (HLA-A*02:01) , His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 28.1ng/ml determined by ELISA.

制剂（Formulation）

Supplied as 0.22μm filtered solution in PBS (pH 7.4).