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Recombinant Human WT-1 (HLA-A*02:01) Protein, His-Avit Tag

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详细介绍

 

Wilms tumor gene 1 (WT1) plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). The Human WT-1 (RMFPNAPYL) (HLA-A*0201) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.

 

性能参数

Synonyms

MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33

Uniprot No.

 A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL

Source

Recombinant Human WT-1 (HLA-A*02:01) Protein is expressed from HEK293 cells with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide.

Molecular Weight

The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

<1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Dissolve lyophilized protein with sterile water to ensure the concentration is greater than 100 ug/mL.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

产品数据

Ver. CN20230419

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