Recombinant Human CD83 Protein,His Tag

表达区间及表达系统（Source）

Recombinant Human CD83 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Thr20-Ala143.[Accession | Q01151-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 15.2 kDa. Due to glycosylation, the protein migrates to 18 kDa and 25-28 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

活性（Activity）

ELISA Data:  Immobilized Human CD83, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA. 

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.