Recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) ,His Tag

分子别名（Synonyms）

CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18

表达区间及表达系统（Source）

Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus. It contains Gln22-Asp117.[Accession | Q95LI5]

分子量大小（Molecular Weight）

The protein has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 13-16 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Anti-CD3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.