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Recombinant Biotinylated Cynomolgus IL-2R beta/CD122 Protein (Primary Amine Labeling) ,His Tag

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详细介绍

性能参数

 

表达区间及表达系统(Source)

Biotinylated Cynomolgus IL-2 R beta/CD122 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminal.

It contains Ala27-Asp239.[Accession | Q38J85]

分子量大小(Molecular Weight)

The protein has a predicted MW of 25.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 90% as determined by HPLC

 

           活性(Activity)

SPR Data: Biotinylated Cynomolgus IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-2, No Tag with an affinity constant of 0.148 μM as determined in SPR assay

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

 

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

 

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.    

 

Ver. CN20230620

     

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