Recombinant Human Neuritin Protein

Synonyms

NRN1

Accession

Q9NPD7

GeneID

51299

Source

E.coli-derived Human Neuritin, Ala28-Asn115.

Molecular Weight

Approximately 19.4 kDa.

AA Sequence

AGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGN

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity

The ED₅₀ as determined by by a cell proliferation assay using rat C6 cells is less than 25 ng/mL, corresponding to a specific activity of > 4.0 × 10⁴ IU/mg. Fully biologically active when compared to standard. 

Endotoxin

< 0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.