Recombinant Human NT-4 Protein

Product Name

Cat#

Size

Recombinant Human NT-4 Protein

92114ES05

2 μg

92114ES10

10 μg

92114ES50

50 μg

92114ES60

100 μg

Synonyms

Neurotrophin-5, NT-5; Neurotrophin-4 ;GLC10

Accession

P34130

GeneID

4909

Source

E.coli-derived Human NT-4, G81-A210, with an N-terminal Met.

Molecular Weight

Approximately 28.1 kDa.

AA Sequence

MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity

The ED₅₀ as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/mL, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg. Fully biologically active when compared to standard. 

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.5.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.