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Recombinant Human FGFR2 beta (IIIb) Domain Protein,hFc Tag

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详细介绍

性能参数

表达区间及表达系统(Source

Human FGFR2 beta (IIIb) Domain Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Pro253-Glu378.[Accession | P21802-3]

分子量大小(Molecular Weight

The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin

Less than 1EU per μg by the LAL method.

纯度(Purity

> 95% as determined by Tris-Bis PAGE and HPLC

活性(Activity

ELISA Data: Immobilized Human FGFR2 beta (IIIb) Domain, hFc Tag at 0.5μg/ml (100μl/Well)

on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody,hFc Tag with the EC50 14.3ng/ml determined by ELISA.

制剂(Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

Ver. CN20230625

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