Recombinant Human FGFR2 beta (IIIb) Protein,His Tag

蛋白别名（Synonyms）

Fibroblast growth factor receptor 2;FGFR-2;KGFR;CD332

表达系统及表达区间（Source）

Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg152-Glu378.[Accession | P21802-3]

分子量（Molecular Weight）

The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

< 1EU per μg by the LAL method.

活性（Activity）

Blocking Data: Serial dilutions of Anti-FGFR2 Antibody were added into Human FGFR2 beta (lllb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 1.74ng/ml.

ELISA Data: Immobilized Human FGFR2 beta (IIIb) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR2 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.

SPR Data: Anti-FGFR2 Antibody, hFc Tag can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 1.98nM as determined in a SPR assay .

纯度（Purity）

> 95% as determined by SDS-PAGE and by HPLC.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

复溶方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.