Recombinant Human CD3E/CD3 epsilon monomer Protein,hFc Tag

表达区间及表达系统（Source）

Human CD3E/CD3 epsilon monomer Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Asp23-Asp126(C119S, C122S).[Accession | P07766]

分子量大小（Molecular Weight）

The protein has a predicted MW of 36.1 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on SDS- PAGE result.

内毒素（Endotoxin）

<1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS- PAGE

活性（Activity）

ELISA Data: Immobilized Human CD3E, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 33.5ng/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.