Recombinant Biotinylated Human CD3E/CD3 epsilon 1-27 peptide Protein,hFc-Avi Tag

表达区间及表达系统（Source）

Biotinylated Human CD3E/CD3 epsilon 1-27 peptide Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-terminal. It contains Asp23-Thr48.[Accession | P07766]

分子量大小（Molecular Weight）

The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

ELISA Data: Immobilized Anti-CD3 Antibody, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD3E 1-27 peptide, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. 

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.