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Recombinant Human CD117 Protein, His Tag

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详细介绍

 

c-Kit /CD117, is a receptor tyrosine kinase. Binding of SCF R to SCF, promotes the survival, differentiation, and mobilization of progenitor cells in multiple lineages. Mutations or deletions of SCF R cause a wide variety of malignancies as well as pigmentation disorders and sterility. Within the ECD, human SCF R shares 73% and 76% aa sequence identity with mouse and rat SCF R, respectively.

 

产品性质

Synonyms

SCFR; PBT; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT

Uniprot No.

P10721.2

Source

Recombinant Human CD117 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gln26-Thr516.

Molecular Weight

Approximately 56.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

The products are shipped with ice pack and can be stored at -20 for 1 year.

2-7 days, 2 to 8 °C under sterile conditions after reconstitution.
3-6 months, -20 to -80°C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

 

注意事项

1.For your safety and health, please wear lab coats and disposable gloves for operation.

2.For research use only!

 

产品数据

 

 

HB22122

 

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