ITC表征酶动力学

All enzymatic reactions generate heat, so all enzymatic reactions can in principle be directly studied by calorimetry. Calorimetric experiments do not require extensive protocol development, and measurements are direct, rapid, non-destructive and sensitive. In addition to kinetics data, ITC also generates thermodynamic information which can help correlate enzyme stability to reaction rates and substrate specificity. Affinity 等温滴定微量热仪