一种使用飞秒激光器用于稳定和固定钙粘蛋白的双重机械装

Epithelial tissues maintain a robust architecture which is important for their barrier function, but they are also remodelled through the reorganization of cellcell contacts. Tissue stability requires intercellular adhesion mediated by E-cadherin, in particular its trans-association in homophilic complexes supported by actin filaments through b- and a-catenin. How a-catenin dynamic interactions between E-cadherin/b-catenin and cortical actin control both stability and remodel领 of adhesion is unclear. Here we focus on Drosophila homophilic E-cadherin complexes rather than total E-cadherin, including diffusing free E-cadherin, because these complexes are a better proxy for adhesion. We find that E-cadherin complexes partition in very stable microdomains (that is, bona fide adhesive foci which are more stable than remodel领 contacts). Furthermore, we find that stability and mobility of these microdomains depend on two actin populations: small, stable actin patches concentrate at homophilic E-cadherin clusters, whereas a rapidly turning over, contractile network constrains their lateral movement by a tethering mechanism. a-Catenin controls epithelial architecture mainly through regulation of the mobility of homophilic clusters and it is largely dispensable for their stability. Uncoup领 stability and mobility of E-cadherin complexes suggests that stable epithelia may remodel through the regulated mobility of very stable adhesive foci. 飞秒激光器（飞秒光纤激光器）