用户论文：A methylene blue-mediated enzyme electrode

全文请访问：http://www.spm.com.cn/papers/p95.pdf
A methylene blue-mediated enzyme biosensor has been developed for the detection of inhibitors including mercury(II),mercury(I), methylmercury, and mercuryglutathione complex. The inhibition to horseradish peroxidase was apparently reversible and noncompetitive in the presence of HgCl2 in less than 8 s and irreversibly inactivated when incubated with different concentrations of HgCl2 for 18 min. The binding site of horseradish peroxidase with HgCl2 probably was a cysteine residue SH. Mercury compounds can be assayed amperometrically with the detection limits 0.1 ng ml 1 Hg for HgCl2 and methylmercury,0.2 ng ml 1 Hg for Hg2(NO3)2 and 1.7 ng ml 1 Hg for mercuryglutathione complex. Inactivation of the immobilized horseradish peroxidase was displayed in the AFM images of the enzyme membranes. 全文请访问：http://www.spm.com.cn/papers/p95.pdf 扫描探针显微镜(SPM/AFM/STM)