Column:TSKgel ODS-100V (I.D.4.6 mm×150 mm, 3m)
Eluent: 20 mmol / L ammonium acetate 0.15% (v / v) trifluoroacetic acid
aqueous solution (pH 3.4) - ACN (95:5)
Flow rate:0.2 mL/min
Column temperature:room temperature
Detector:ELSD
Detector parameter : Gas flow rate:2.5 L/min; Evaporation temperature :50
Sample: streptomycin 50 mg/ml, Dihydrostreptomycin 40 mg/ml, Tobramycin 20 mg/ml
Injection vol.: 10 L 高性能反相色谱分析柱TSKgel ODS-100V/100Z
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