运用高速逆流色谱法从太子参中分离纯化Pseudostellarin

Abstract： Pseudostellarin B (cyclic peptide) was isolated and purified from the herbs of Pseudostellaria heterophylla (Miq.)Pax for the first time by high-speed counter-current chromatography(HSCCC)using a two-phase solvent system consisting of n-butanol-ethyl acetate-water(0.6:4.4:5,v/v).The technique can isolate mg levels of the target compound per run with purity better than 96%. The chemical structure of the compound has been positively confirmed by electrospray ionization time of flight(TOF) MS,H-NMR,C-NMR and H-C COSY analyses. TBE-300A高速逆流色谱仪/制备色谱仪 TBE-1000A制备色谱仪/萃取仪/快速分离制备色谱仪 TBE300B+AKTA高速逆流色谱仪/离心分配色谱/萃取仪/制备色谱仪 TBE-200V 高速逆流色谱仪 TBE-20A分析型高速逆流色谱仪/萃取仪/制备色谱仪