TMEM16A

TMEM16A是一种钙激活的氯离子通道，表达于气道表面上皮、粘膜下腺体和杯状细胞中。它在炎症状态下上调。最近，TMEM16A的一种正性调节剂已被证明可增强囊性纤维化患者的阴离子和液体分泌。这种方法为囊性纤维化和其他呼吸系统疾病的研究提供了一种新的方法。
Key Publication：
Danahay, H.L., Lilley, S., Fox, R., et al. 2019. TMEM16A Potentiation: A Novel Therapeutic Approach for the Treatment of Cystic Fibrosis. American Journal of Respiratory and Critical Care Medicine

Measuring TMEM16A

TMEM16A Channels can be measured using automated patch clamp when expressed in cell lines, and can be activated using the internal perfusion feature of the SyncroPatch 384i or using the Orbit instruments when they are recombinantly expressed in bilayers.

High throughput automated patch clamp

Parallel bilayer recordings

Cytotoxicity measurements

Application Notes

TMEM16A (ANO1) - "Internal perfusion of Ca2+ to activate TMEM16A/ANO1 on the SyncroPatch 384PE"