碱裂解法提取质粒后电泳拖尾严重，是什么原因

刘署建 2016-03-20 12:34:49 847 浏览

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baby墨情86 2016-03-21 00:00:00

和marker平齐的是完整质粒，后面的应该是开环DNA，整体挺清楚的，也没有滞后或者拖尾的现象但是1和2亮度明显低证明质粒的量比3和4少

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习惯成自然PAP 2016-03-29 00:00:00

拖尾严重的话说明质粒有降解，形成不同大小的片段，条带特异性差

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sunmoon9927 2017-11-22 00:00:00

现在实验室大多用的是提取试剂盒，如果自制溶液碱性过强会有可能出现拖尾现象，蛋白沉降步骤不完全也会有可能导致基因组DNA降解出现拖尾现象。类似于溶液方面的问题一般比较容易排除，而且比较容易改正。电泳结果拖尾大部分原因与实验操作有关，在加入碱性溶液裂解细胞后操作需要轻柔，静置时间不宜过长，剧烈震荡以及静置时间过长均可导致质粒DNA断裂，电泳结果中出现拖尾现象。有什么问题可以继续追问，欢迎交流。

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