碱裂解法制备质粒dna时，溶液1，2，3中各组分都起什么作用

艾轮吧 2015-04-16 02:52:27 1274 浏览

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追天揽月123 2015-04-17 00:00:00

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面瘫是班头啊 2017-11-29 00:00:00

加入溶液1的目的主要是将细菌团块重新溶解于缓冲液中；溶液2含有SDS及NaOH，前者可将细菌溶解，后者可将DNA变性；溶液3是由醋酸和钾盐组成，前者在于中和碱性，后者则有利于染色体DNA的沉淀

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碱裂解法制备质粒dna时，溶液1，2，3中各组分都起什么作用:

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