Recombinant Human GDF15 Protein,His Tag

蛋白别名（Synonyms）

GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta;

种属（Species)

Human

表达系统（Source）

E.coli

表达序列（Sequence）

Ala197-Ile308

Accession

Q99988

标签（Tag)

N-His

分子量（Molecular Weight）

The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Bis-Tris PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Bis-Tris PAGE

活性（Activity）

ELISA Data: Immobilized Human GDF15, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA.

Blocking Data: Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.

SPR Data: Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay .

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.

外观（Appearance）

冻干粉