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Recombinant Cynomolgus IL-1 Beta/IL-1F2 Protein,His Tag
性能参数
|
表达区间及表达系统(Source) |
Biotinylated Human M-CSF/CSF-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu33-Asn190.[Accession | P09603-1] |
|
分子量大小(Molecular Weight) |
The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Tris-Bis PAGE result. |
|
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
|
纯度(Purity) |
> 95% as determined by Tris-Bis PAGE and HPLC |
|
活性(Activity) |
ELISA Data: Immobilized Human M-CSF R, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human M-CSF, His Tag with the EC50 of 19.4ng/ml determined by ELISA. |
|
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
重构方法(Reconstitution) |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20231129
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